Effects of Medicinal Plant Extracts as Pre-milking Teat Dip to Reduce Teat-end Bacterial Load in Lactating Dairy Cow

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Pranpreya Todtong


The aim of this experiment was study the effects of medicinal plant extracts as pre-milking teat dip to reduce teat-end bacterial load in lactating dairy cow. The experimental design was a Completely Randomized Design (CRD) of 6 treatments follow as types of medicinal plant extracts such as Curcuma longa L., Nymphaea lotus L. (flower pollen), Nymphaea lotus L. (flower petals), Garcinia mangostana L., Durio zibethinus L. and Psidium guajava. L. The medicinal plant extraction by maceration method using 70 % methanol and drying by Freeze Dryer. Antibacterial activity testing of medicinal plant extracts by MIC and MBC. The efficacy testing of pre-milking teat dip solution in lactating dairy cows by comparing the total bacterial count on teat end before and after dipping the solution. The results of the study found that flower pollen of Nymphaea lotus L. (38.40 %) was highest % yield. The results of antibacterial activity testing found that Psidium guajava. L. extract was the most effective in inhibiting bacteria. The MIC and MBC values were against S. aureus (0.25 and 1 mg/ml) and E. coli (0.5 and 2 mg/ml). And the results of the efficacy of pre-milking teat dip solution found that the extraction of Curcuma longa L., Nymphaea lotus L. (flower pollen), Nymphaea lotus L. (flower petals), Durio zibethinus L. and Psidium guajava. L. was a statistically significant difference in reducing the total bacterial count of teat end (P<0.05). In conclusion, extracts from Curcuma longa L., Nymphaea lotus L. (flower pollen), Nymphaea lotus L. (flower petals), Durio zibethinus L. and Psidium guajava. L. can be applied as a pre-milking teat dip to reduce the teat end bacterial load in lactating dairy cows.


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Abinaya, P., and S. Thangarasu. 2017. Testing the efficacy of potassium permanganate as an antiseptic agent for the control of bovine mastitis. Int. J. Curr. Microbiol. App. Sci. 6(10): 609-611.

Barnum, D.A., R.E. Johnson, and B.W. Brooks. 1982. An evaluation of a teat dip with dodecyl benzene sulfonic acid in preventing bovine mammary gland infection from experimental exposure to Streptococcus agalactiae and Staphylococcus aureus. Can Vet J. 23(2): 50-54.

Boddie, R.L., and S.C. Nickerson. 2002. Reduction of mastitis caused by experimental challenge with Staphylococcus aureus and Streptococcus agalactiae by use of a quaternary ammonium and halogen-mixture teat dip. J. Dairy Sci. 85(1): 258-262.

Bogni, C., L. Odierno, C. Raspanti, J. Giraudo, A. Larriestra, E. Reinoso, M. Lasagno, M. Ferrari, E. Ducrós, C. Frigerio, S. Bettera, M. Pellegrino, I. Frola, S. Dieser, and C. Vissio. 2011. War against mastitis: Current concepts on controlling bovine mastitis pathogens, in A. Méndez-Vilas (ed.), Science against microbial pathogens: Communicating current research and technological advances. 483–494, Rio Cuarto, Cordoba, Argentina.

Botosoglou, N.A., and D.J. Fletouris. 2001. Drug Residues in Foods: Pharmacology, Food Safety and Analysis. Marcel Dekker inc. ed.NY. pp. 1-15.

Chen, G., Y. Li, W. Wang, and L. Deng. 2018. Bioactivity and pharmacological properties of α-mangostin from the mangosteen fruit: a review. Expert. Opin. Ther. Pat. 28: 415–427.

Das, M., and S. Goswami. 2019. Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: Role of phytochemicals. Int. J. Health. Sci. Res. 9: 39.

Dhiman, A., A. Nanda, S. Ahmad, and B. Narasimhan. 2011. In vitro antimicrobial activity of methanolic leaf extract of Psidium guajava L. J. Pharm. Bioallied. Sci. 3: 226–229.

Dore, S., A.M. Ferrini, B. Appicciafuoco, M.R. Massaro, G. Sotgiu, M. Liciardi, and E.A. Cannas. 2019. Efficacy of a terpinen-4-ol based dipping for post-milking teat disinfection in the prevention of mastitis in dairy sheep. J. Essent. Oil Res. 31(1): 19-26.

Gleeson, D., B. O’Brien, J. Flynn, E. O’ Callaghan, and F. Galli. 2009. Effect of pre-milking teat preparation procedures on the microbial count on teats prior to cluster application. Ir. Vet. J. 62(7): 461-467.

Hassan, K.J., S. Samarasinghe, and M.G. Lopez-Benavides. 2009. Use of neural networks to detect minor and major pathogens that cause bovine mastitis. J. Dairy. Sci. 92(4): 1493-1499.

Hemling, T.C. 2002. Teat Condition - Prevention and Cure Through Teat Dips Proceedings of the British Mastitis Conference (2002) Brockworth, p 1-14 Institute for Animal Health/Milk Development Council.

Hogeveen, H., K. Huijps, and T.J.G.M. Lam. 2011. Economic aspects of mastitis: New developments. N. Z. Vet. 59: 16–23.

Hohmann M-F., N. Wente, Y. Zhang, and V. Krömker. 2020. Bacterial Load of the Teat Apex Skin and Associated Factors at Herd Level. Animals. 10(9): 1647.

Izquierdo, A.C., J.E.G. Liera, R.E. Cervantes, J.F.I. Castro, E.A.V. Mancera, R.H. Crispín, M. L.J. Mosqueda, A.G. Vázquez, J.O. Pérez, P.S. Aparicio, and B.E.R. Denis. 2017. Production of milk and bovine mastitis. J. Adv. Dairy Res. 5(2): 1-4.

Jassal, K., and S. Kaushal. 2019. Phytochemical and antioxidant screening of guava (Psidium guajava) leaf essential oil. Agric. Res. J. 56: 528.

Julianto, J., P. Sambodho, and D.W. Harjanti. 2017. Pengaruh dipping menggunakan ekstrak daun belimbing wuluh (Averrhoa bilimbi Linn.) terhadap total bakteri dan jamur susu sapi perah mastitis subklinis. Agromedia. 35(1): 7-13

Kamal, R.M., and M.A. Bayoumi. 2015. Efficacy of premilking and postmilking teat dipping as a control of subclinical mastitis in Egyptian Dairy cattle. Int. Food. Res. J. 22(3): 1037-1042

Kummee, P., M. borisutpeth, S. chanlun, P. kanbutra, and A. chanlun. 2015. Efficacy of guava leaf extract as alternative pre-milking teat dipping in reducing teat–end bacterial load of milking dairy cows. Int. J. Pharm. Pharm. Sci. 7(9): 434-438

Leslie, K.E., E. Vernooy, A. Bashiri, and R.T. Dingwell. 2006. Efficacy of two hydrogen peroxide teat disinfectants against Staphylococcus aureus and Streptococcus agalactiae. J. Dairy Sci. 89(9): 3696-3701.

Mabona, U., A. Viljoen, E. Shikanga, A. Marston, and S. Van Vuuren. 2013. Antimicrobial activity of southern African medicinal plants with dermatological relevance: From an ethnopharmacological screening approach, to combination studies and the isolation of a bioactive compound. J. Ethnopharmacol. 148: 45–55.

Mahpudin, F. Wahyono, and D.W. Harjanti. 2017. Efektivitas ekstrak daun babadotan sebagai green antiseptic untuk pencelup puting sapi perah. Agripet. 17 (1): 15-21.

Majic, B., B.V. Jovanovic, Z. Ljubic, and S. Kukovics. 1993. Typical problems encountered in Croatia in the operation of goats milking machines. Proceedings of the 5th Internacional symposium on machine milking of small ruminants. Budapest, Hungary. 377-379.

Makovec, J.A., and P.L. Ruegg. 2003. Results of Milk Samples Submitted for Microbiological Examination in Wisconsin from 1994 to 2001. J. Dairy. Sci. 86: 3466–3472.

Manimekalai I., K. Sivakumari, K. Ashok, and S. Rajesh. 2016. Phytochemical profiling of mangosteen fruit, Garcinia mangostana. W. J. Pharm. Pharm. Sci. 5(2): 221-252.

Mehan, S., S. Kalra, R. Singh, M. Mehta, and D. Khanna.2014. Possible protective strategies of “queen of tropical fruits”: Garcinia mangostana in age related neurodegenerative disorder like Huntington’s disease. J. Neurol. Neurophysiol. 5(5): 116-118.

Mickymaray, S. 2019. Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens. Antibiotics. 8: 257.

Monsallier, F., I. Verdier-Metz, C. Agabriel, B. Martin, and M.C. Montel. 2012. Variability of microbial teat skin flora in relation to farming practices and individual dairy cow characteristics. Dairy. Sci. Technol. 92: 265–278.

Morais-Braga, M.F.B., Carneiro, J.N.P., Machado, A.J.T., dos Santos, A.T.L., Sales, D.L., Lima, L.F., Figueredo, F.G., Coutinho, H.D.M., 2016a. Psidium guajava L., from ethnobiology to scientific evaluation: Elucidating bioactivity against pathogenic microorganisms. J. Ethnopharmacol. 194, 1140–1152.

Nickerson, S.C., J.L. Watts, R.L. Boddie, and J.W. Pankey. 1986. Evaluation of .5% and 1% Iodophor teat dips on commercial dairies. J. Dairy. Sci. 69(6): 1693-1698.

Oghenejobo, M., O. Opajobi, U. Oghenejobo Bethel, and U. Uzoegbu. 2017. Antibacterial evaluation, phytochemical screening and ascorbic acid assay of turmeric (Curcuma longa). MOJ. Bioequiv. Availab. 4(2): 232-239

Okwu, M.U., M. Olley, A.O. Akpoka, and O.E. Izevbuwa. 2019. Methicillin-resistant Staphylococcus aureus (MRSA) and anti-MRSA activities of extracts of some medicinal plants: A brief review. AIMS. Microbiol. 5: 117–137.

Oliver, S.P., B.E. Gillespie, M.J. Lewis, S.J. Ivey, R.A. Almeida, D.A. Luther, D.L. Johnson, K.C. Lamar, H. Moorehead, and H.H. Dowlen. 2001. Efficacy of a premilking teat disinfectant containing a phenolic combination for the prevention of mastitis. J. Dairy. Sci. 84(6): 1545-1549.

Oliver, S.P., B.E. Gillespie, M.J. Lewis, S.J. Ivey, R.A. Almeida, D.A. Luther, D.L. Johnson, K.C. Lamar, H. Moorehead, and H.H. Dowlen. 2001. Efficacy of a premilking teat disinfectant containing a phenolic combination for the prevention of mastitis. J. Dairy. Sci. 84(6): 1545-1549.

Oliver, S.P., M.J. Lewis, T.L. Ingle, B.E. Gillespie, K.R. Matthews, and H.H. Dowlen. 1993. Premilking Teat Disinfection for the Prevention of Environmental Pathogen Intramammary Infections. J. Food. Protec. 56(10): 852-855.

Purwantiningsih, T.I., Y.Y. Suranindyah, and Widodo. 2017. Efektivitas celup puting menggunakan ekstrak buah mengkudu (Morinda citrifolia) terhadap hasil uji California Mastitis Test (CMT). Sains. Peternakan. 15 (2): 66-69.

Sah, BP., T. Pathak, DS. Sankar, and DB. Suresh. 2014. Phytochemical Investigations on the Fruits of Durio zibenthinus Linn. For Antimicrobial Activity. Int. J. Pharm. Sci. Res. 5(12): 878-891.

Samad, M.A. 2008. Animal Husbandry and Veterinary Science, volume II, LEP pub no.11, Bangladesh Agricultural University campus, Mymensingh.

Sampimon, O.C., J.C. Vernooij, and J. Sol. 2004. Practical aspects at drying-off. Tijdschrift Voor Diergeneeskunde. 129: 828-833.

Schultze, W.D., and J.W. Smith. 1972. Effectiveness of postmilking teat dips. J. Dairy. Sci. 55(4): 426-431.

Seddiek, A.S., G.M. Hamad, A.A. Zeitoun, M.A.M. Zeitoun, and S. Ali. 2020. Antimicrobial and antioxidant activity of some plant extracts against different food spoilage and pathogenic microbes. Eur. J. Nutr. Food. Saf. 12: 1–12.

Seegers, H., C. Fourichon, and F. Beaudeau. 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Vet. Res. 34: 475–491.

Smith, K.L., and J.S. Hogan. 1993. Environmental mastitis. The Veterinary Clinics of North America Food Animal Practice. 9: 489-498.

Soliman, F.M., M.M. Fathy, M.M. Salama, and F.R. Saber. 2016. Comparative study of the volatile oil content and antimicrobial activity of Psidium guajava L. and Psidium cattleianum Sabine leaves. Bull. Fac. Pharm. Cairo Univ. 54: 219–225.

Sundaram, B., C. Gopalakrishnan, S. Subramanian, D. Shankaranarayanan, and L. Kameswaran. 1983. Antimicrobial activities of Garcinia mangostana. Planta .Med. 48: 59–60.

Todtong. P., T. Nilnont, and R. Pilachai. 2021. Prevalence and antimicrobial susceptibility on bacterial pathogens of clinical and subclinical mastitis in lactating cows in Udon Thani Province. KKU. Vet. J. 31(1): 29-37.

Vissers, M.M.M., and F. Driehuis. 2009. On-Farm Hygienic Milk Production. In Tamime, A.Y. (Ed.). Milk processing and quality management. (pp. 1-17). USA: Wiley-Blackwell Publisher.

Voravuthikunchai, SP., and L. Kitpipit. 2005. Activity of medicinal plant extracts against hospital isolates of Methicillin-resistant Staphylococcus aureus (MRSA). Clin. Microb. Infect. 11: 493–512.

Wente, N., V. Zoche-Golob, M. Behr, and V. Krömker. 2016. Susceptibility to cephalosporins of bacteria causing intramammary infections in dairy cows with a high somatic cell count in Germany. Prev. Vet. Med. 131:146–151.

Yanuartono, Y., A. Nururrozi, S. Indarjulianto, H. Purnamaningsih, and D. Ramandani. 2020. The Benefits of Teat Dipping as Prevention of Mastitis. Journal of Livestock Science and Production. 4(1): 213-249.

Yasothai, R. 2017. Effect of premilking and postmilking teat dipping in control of subclinical mastitis in dairy cattle. Int. J. Sci. (2): 1413-1417.

Yisa, J. 2009. Phytochemical analysis and antimicrobial activity of Scoparia dulcis and Nymphaea Lotus. Aust. J. Basic. Appl. Sci. 3(4): 3975-3979.