The Molecular Detection of Leptospira spp. in Domestic Cats from Small Animal Hospital, Chiang Mai, Thailand.
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Abstract
Domestic cats can transmit Leptospira spp. and may play an important role in human infection. The aim of this study was to detect Leptospiral infection in mature cats presented to Small Animal Veterinary, Chiang Mai University, by using immunofluorescence assay (IFA) and real-time PCR (RT-PCR) techniques. Eighty mature cat serum and urine were collected. The results showed that all of IFA's serological results were negative. Otherwise, we found 2.5 percent of outdoor cats (2/80) positive with RT-PCR, with one cat positive in urine and another positive in urine and serum from the Muang and Doi Tao areas, respectively. This study discovered the presence of Leptospira spp. in mature cats brought to Small Animal Veterinary, Chiang Mai University, implying that outdoor cats could be an important reservoir for the transmission of Leptospira bacteria. Furthermore, more research is needed to investigate the role of transmission in the cat and to examine the disease prevention rule not only for the owner but also for staffs in animal hospitals.
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References
Alashraf, A.R., Lau, S.F., Khairani-Bejo, S., Khor, K.H., Ajat, M., Radzi, R., Roslan, M.A., & Abdul Rahman, M. S. 2020. First report of pathogenic Leptospira spp. isolated from urine and kidneys of naturally infected cats. PloS one, 15(3), e0230048.
Arbour, J., Blais, M.C., Carioto, L., Sylvestre, D. 2012. Clinical leptospirosis in three cats (2001–2009). J. Am. Anim. Hosp. Assoc. 4, 256–260.
Bourhy, P., Bremont, S., Zinini, F., Giry, C., Picardeau, M. 2011. Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences. J. Clin. Microbiol. 49, 2154–2160.
Brasil de Lima, A.W., Parantoni, R.N., Feitosa, T.F., Vilela, V.L.R., Alves, C.J., Vasconcellos, S.A., & de Azevedo, S. S. 2014. Anti‐Leptospira spp. antibodies in cats from the semiarid of the Paraíba State. Semin. Ciênc. Agrá. 35, 3215–3220
Chadsuthi, S., Bicout, D.J., Wiratsudakul, A., Suwancharoen, D., Petkanchanapong, W., Modchang, C., Chalvet-Monfray, K. 2017. Investigation on predominant Leptospira serovars and its distribution in humans and livestock in Thailand, 2010-2015. PLoS. Negl. Trop. Dis. 11(2), e0005228.
Chan, K.W., Hsu, Y.H., Hu, W.L., Pan, M.J., Lai, J.M., Huang, K.C., Chou, S.J. 2014. Serological and PCR detection of feline leptospira in southern Taiwan. Vector. Borne. Zoonotic. Dis. 14,118-123.
Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., Ko, A.I. 2015. Global Morbidity and Mortality of Leptospirosis: A Systematic Review. PLoS. Negl. Trop. Dis. 9(9), e0003898.
Dorsch, R., Ojeda, J., S.algado, M., Monti, G., Collado, B., Tomckowiack, C., Tejeda, C., Müller, A., Eberhard, T., Klaasen, H., & Hartmann, K. (2020). Cats shedding pathogenic Leptospira spp.-An underestimated zoonotic risk?. PloS one., 15(10), e0239991.
Dybing, N.A., Jacobson, C., Irwin, P., Algar, D., Adams, P.J. 2017. Leptospira Species in Feral Cats and Black Rats from Western Australia and Christmas Island. Vector. Borne. Zoonotic. Dis. 17, 319-324.
Ellis, W.A., 2015. Animal leptospirosis. In: Adler B, editor. Leptospira and Leptospirosis. Berlin: Springer. 99–137.
Gamage, C.D., Yasuda, S.P., Nishio, S. 2011. Serological evidence of Thailand virus-related hantavirus infection among suspected leptospirosis patients in Kandy, Sri Lanka. Jpn. J. Infect. Dis. 64, 72–75.
Green, C.E., Sykes, J.E., Moore, G.E., Golstein, R.E. 2011. Leptospirosis. In: Green, CE, ed. Infectious Disease of Dog and Cat. Philadelphia: Saunders Elsevier. 431–447
Haake, D.A., Levett, P.N. 2015. Leptospirosis in Humans. Curr. Top. Microbiol. Immunol. 387, 65–97.
Hartskeerl, R.A., Collares-Pereira, M., Ellis, W.A. 2011. Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. Clin. Microbiol. Infect. 17, 494-501.
Hinjoy, S. 2016. Epidemiology of leptospirosis from Thai national disease surveillance system, 2003-2012. OSIR. 7, 1–5.
Lapointe, C., Plamondon, I., Dunn, M. 2013. Feline leptospirosis serosurvey from a Quebec referral hospital. Can. Vet. J. 54, 497-499.
Larsson, C. E., Santa Rosa, C. A., Hagiwara, M. K., Paim, G. V., & Guerra, J. L. 1984. Prevalence of feline leptospirosis: Serologic survey and attempts of isolation and demonstration of the agent. Int. J. Zoonoses. 11, 161–169.
Levett, P.N., 2015. Systematics of Leptospiraceae. In: Adler B, editor. Leptospira and Leptospirosis. Berlin: Springer. 11–20.
Llewellyn, J.R., Krupka-Dyachenko, I., Rettinger, A.L., Dyachenko, V., Stamm, I., Kopp, P.A. 2016. Urinary shedding of leptospires and presence of Leptospira antibodies in healthy dogs from upper Bavaria. Berl. Münch. Tierärztl. Wochenschr. 129, 251–257.
Markovich, J.E., Ross, L., McCobb, E. 2012. The prevalence of leptospiral antibodies in free roaming cats in Worcester County, Massachusetts. J. Vet. Intern. Med. 26, 688–689.
Meeyam, T., Tablerk, P., Petchanok, B., Pichpol, D., Padungtod, P. 2006. Seroprevalence and risk factors associated with leptospirosis in dogs. Southeast. Asian. J. Trop. Med. Public. Health. 37, 148–153.
Millán, J., Candela, M.G., López-Bao, J.V., Pereira, M., Jiménez, M.A., León-Vizcaino, L. 2009. Leptospirosis in wild and domestic carnivores in natural areas in Andalusia, Spain. Vector. Borne. Zoonotic. Dis. 9, 549–554.
Mosallanejad, B., Ghorbanpoor Najafabadi, M., Avizeh, R., Abdollahpoor, G., Abadi, K. 2011. A serological survey of Leptospiral infection of cats in Ahvaz, south‐western of Iran. Iran. J. Vet. Med. 5, 49–52.
Mylonakis, M.E., Bourtzi-Hatzopoulou, E., Koutinas, A.F. 2005. Leptospiral seroepidemiology in a feline hospital population in Greece. Vet Rec. 156, 615–616.
Podgoršek, D., Ružić-Sabljić, E., Logar, M. 2020. Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection. BMC Microbiol. 20, 59.
Sprißler, F., Jongwattanapisan, P., Luengyosluechakul, S., Pusoonthornthum, R., Prapasarakul, N., Kurilung, A., Goris, M., Ahmed, A., Reese, S., Bergmann, M., Dorsch, R., Klaasen, H.L.B.M., Hartmann, K. 2018. Leptospira Infection and Shedding in Cats in Thailand. Transbound. Emerg. Dis. 66 , 948-956.