Investigation of Differential Somatic Cell Count as Supplementary Indicator to Somatic Cell Count for Identification of Subclinical Mastitis in Dairy Cows
Article Sidebar
Main Article Content
Abstract
In dairy farms, somatic cell count (SCC) is widely used to determine subclinical mastitis and milk quality. Recently, more studies have been applying differential somatic cell count (DSCC) as a tool to identify subclinical mastitis. DSCC represents the total polymorphonuclear leukocytes (PMN) and lymphocytes expressed as a percentage of the total. This study aimed to investigate the relationship between DSCC and SCC in the assessment of subclinical mastitis. We collaborated with 4 farms totaling 140 dairy cows. Intramammary infection (IMI) or mastitis status was assessed using microbiological cultures and biochemical tests for major and minor pathogens. The milk was analyzed for SCC and DSCC using Fossomatic 7DC. The result of our study showed that the combination of DSCC of 47.35% and SCC 100,00- 120,000 cells/mL classified cows as infected by 53.42% major pathogens and 46.55% minor pathogens. In conclusion, our study supports the new approach based on the combined use of DSCC and SCC to screen for IMI and could be a new indicator for mastitis screening in dairy farms. However, more work on the subject is needed to provide more precise information on the diagnosis of mastitis.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
สุชาติ สุขสถิตย์ นุกูล อินทระสังขา ไชยวรรณ วัฒนจันทร์ วิศิษฐ ทองเที่ยง. 2547. การศึกษาปัจจัยที่เกี่ยวข้องกับการเกิดโรคเต้านมอักเสบและการจัดการด้านสุขศาสตร์ของฟาร์มโคนมในจังหวัดพัทลุง. เข้าถึงได้จาก: http://kb.psu.ac.th/psukb/handle/2553/4552, 14 กุมภาพันธ์ 2566.
สุวิมล พันธุ์ดี และ เอกชัย สร้อยน้ำ. 2546. คู่มือการตรวจสอบคุณภาพน้ำนม. เอกสารเผยแพร่ทางวิชาการ โครงการเพิ่มผลผลิตน้ำนมดิบเพื่อลดการนำเข้าโรงพยาบาลสัตว์มหาวิทยาลัย เกษตรศาสตร์ หนองโพ คณะสัตวแพทยศาสตร์ มหาวิทยาลัยเกษตรศาสตร์. 73 หน้า.
Damm, M., Holm, C., Blaabjerg, M., Novak M.B., Schwarz, D. 2017. Differential somatic cell count-A novel method for routine mastitis screening in the frame of Dairy Herd Improvement testing programs. J Dairy Res. 100(6): 4926-4940.
Kirkeby, C., Toft, N., Schwarz, D., Farre, M., Nielsen, S.S., Zervens, L., Hechinger, S. and Halasa, T. 2019. Differential somatic cell count as an additional indicator for intramammary infections in dairy cows. J. Dairy Sci. 103(2): 1759–1775.
Makovec, J.A. and Ruegg, P.L. 2003. Result of milk samples submitted for microbiological examination in Winconsin from 1994 to 2O01. J. Dairy Sci. 86: 3466-3472.
Paape, M., Mehrzad, J., Zhao, X., Detilleux, J., and Burvenich, C. 2002. Defense of the bovine mammary gland by polymorphonuclear neutrophil leukocytes. Journal of mammary gland biology and neoplasia, 7(2), 109–121.
Pillai, S.R., Kunze, E., Sordillo, L.M. and Jayarao, B.M. 2001. Application of Differential Inflammatory Cell Count as a Tool to Monitor Udder Health. J. Dairy Sci. 84(6): 1413–1420.
Schwarz, D., Diesterbeck, U.S., König, S., Brügemann, K., Schlez, K, Zschöck, M., Wolter, W., Czerny, C.P. 2011. Microscopic differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands. J Dairy Res. 78(4): 448–455.
Schwarz, D., Lipkens, Z., Piepers, S., Vliegher, S.D. 2019. Investigation of differential somatic cell count as a potential new supplementary indicator to somatic cell count for identification of intramammary infection in dairy cows at the end of the lactation period, Preventive Veterinary Medicine, Volume 172, 104803.
Schwarz, D., Santschi. E.D, Durocher, J., Lefebvre, D.M. 2020. Evaluation of the new differential somatic cell count parameter as a rapid and inexpensive supplementary tool for udder health management through regular milk recording. Preventive Veterinary Medicine. 105079.
Sharma, N., Singh, N. K. and Bhadwal M. S. 2011. Relationship of Somatic Cell Count and Mastitis: An Overview. Asian-Aust. J. Anim. Sci. 24(3): 429–438.
Stocco, G., Summer, A., Cipolat-Gotet, C., Zanini, L., Vairani, D., Dadousis, C. and Zecconi A. 2020. Differential somatic cell count- A novel method for routine mastitis screening in the frame of Dairy Herd Improvement testing programs. J. Dairy Sci. 100(6): 4926–4940.
Wall, S.K., Wellnitz, O., Bruckmaier, R.M., Schwarz, D. 2018. Differential somatic cell count in milk before, during, and after lipopolysaccharide- and lipoteichoic-acid-induced mastitis in dairy cows. J Dairy Sci. 101(6):5362-5373.
Zecconia, A., Vairanib, D., Cipollaa, M., Rizzib, N. and Zaninib, L. 2018. Assessment of subclinical mastitis diagnostic accuracy by differential cell count in individual cow milk. Ital. J. Anim. Sci. 18(1): 460-465.