In Vitro Propagation of Kaempferia koratensis Picheans., an Endemic Plant of Thailand

Abstract

This research studied a propagation protocol for Kaempferia koratensis Picheans. which is an endangered plant of Thailand using plant tissue culture technique. Microshoot (1 cm long) of K. koratensis was cultured on solid and liquid Murashige and Skoog (MS) supplemented with various concentrations of auxin, cytokinin and their combinations for eight weeks. The results showed that the highest number of shoots and roots were 4.0 shoots/explant and 8.60 roots/explant when the microshoot was cultured on solid MS medium added with 4 mg/l BA plus 0.2 mg/l NAA. In liquid MS medium, the best result for shoot multiplication was 6.0 shoots/explant achieved on MS medium supplemented with 1 mg/l Kinetin, 2 mg/l TDZ and 0.2 mg/l NAA. The in vitro derived plantlets of K. koratensis Pichean, were transplanted to a pot containing soil, sand and soil:sand (1:1) in a greenhouse. The survival rates were 100% when K. koratensis Pichean. was transplanted to all potting mixture.