Phenolic Compounds, antioxidant and anti-bacterial activities from Markhamia stipulate Seem. Flowers extracts
Keywords:
M. stipulate, Antioxidant activity, Antibacterial activity, phytochemical containsAbstract
The edible flower of Markhamia stipulate Seem are widely used in Thai traditional medicine to treat a variety of health issues. The aims of this study were to investigate antioxidant activity and antibacterial of crude extracts from M. stipulate. In the present study, total phenolic compound, antioxidant activities and antibacterial activities of the 50%ethanolic extract from flower of M. stipulate were evaluated. The total phenolic contents was quantified based on the Folin–Ciocalteu method, and the antioxidant activities of the extract were assessed through the DPPH and FRAP assays. The broth dilution method, MIC and MBC were used to evaluate the antibacterial activity of the extract against Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis and Escherichia coli. The results revealed that the total phenolic contents of the extracts was 53.71±0.002 mg of gallic acid equivalent/g of extract. Additionally, the antioxidant activities in terms of IC50 value of the extracts were 43.56±0.044 mg/mL and 38.43±0.013 mg/mL, according to the DPPH and the FRAP assay. Crude extract of M. stipulate flower also demonstrated antibacterial activity against selected bacterial strains, with MIC and MBC values ranging from 1.56 to 200 mg/mL. In summary, the results suggest that flower extract of M. stipulate may serve as valuable sources of bioactive compounds with antioxidant and antibacterial properties.
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