Optimization of Sugar Production from Oil Palm Empty Fruit Bunch by Cellulase from Aspergilus niger C-1184
Keywords:
Oil palm empty fruit bunch, Cellulase, FE-SEM, HPLCAbstract
This research investigated sugar production from oil palm empty fruit bunch. The steam explosion assisted with 3 M NaOH for 30 minutes was used for pretreatment. After pretreatment, the chemical content of pretreated fiber contained 83.73% cellulose 92.33% holocellulose and 19.33% lignin. In fact, raw material before pretreatment contained 78.22% cellulose 97.78% holocellulose and 27.00% lignin. Then the morphology of treated and untreated fiber were brought to observe by Field Emission Scanning Electron Microscopy Analysis (FE-SEM). It found that there were significant differences in the fiber surface morphology for both treated and untreated oil palm empty fruit bunch. The untreated oil palm empty fruit bunch had a smooth surface while the pretreatment ones had a porous surface. In this study, the oil palm empty fruit bunch was converted into sugar by hydrolysis oil palm empty fruit bunch with 10 mg/ml cellulose from Aspergilus niger C-1184. With 30 g of pretreated oil palm empty fruit bunch, It took 12 hours to process which were able to convert into sugar 14088.33 µg/ml. In addition, the sugar was tested by High-Performance Liquid Chromatography (HPLC), it appeared to be glucose.
References
Alvira, P.E. Tomas-Pejo., Ballesteros, M. & Nergro M.J. (2010). Pretreatment technology for an efficient bioethanol production process based on enzymatic hydrolysis: A review. Bioresour. Technol. 101: 4851-4861.
Browing, B.L. (1963). Method in wood chemistry, pp.389-407. Interscience Publishers, New York, London
Chen, M., Zhao, J., & Xia, L., (2008). Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars. Carbohydrate Polymers, 71, 411-415.
Law, K. N., Daud, W. R. W., & Ghazali, A. (2007). Morphological and chemical nature of fiberstrands of oil palm empty-fruit-bunch (OPEFB). BioResources 2 (3), 351-362.
Latip, N.A., Sofian, A.H., Ail, M.F., Ismail, S.N. & Idris, D.M.N.D. (2019). Structural and morphological studies on alkaline pre-treatment of oil palm empty fruit bunch (OPEFB) fiber for composite production. Materials Today: Proceedings, 17, 1105-1111.
Kraisoraphong.C & Wongpanit.S. (2005). Ethanol production from agricultural waste and Agricultural industry. Defence Energy Department & Loxley Public Company Limited.
Kumar, P., Barrett, D.M., Delwiche, M.J. & Stroeve, P. (2009). Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Industrial and Engineering Chemistry Research, 48(8), 3713-3729
McMillan, J.D., (1994). Pretreatment of lignocellulosic biomass. In: Himmel, M.E., Baker, J.O., Overend, R.P. (Eds.), Enzymatic Conversion of Biomass for Fuels Production. American Chemical Society, Washington, DC, pp. 292–324.
Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 31(3):426-428.
Niwaswong. C. & Ruangviriyachai. C. (2012). Production of Cellulosic Ethanol in Thailand. KKU Sci. J. 40(4). 1073-1088.
Romos, L. P. (2003). The chemical involved in the steam treatment of lignocellulosic material. Quimica Nova, 26(6), 863-871.
Pawongrat. R. (2015). Pretreatment processes for enhancing the efficiency of ethanol production from lignocellulosic agricultural wastes. Veridian E-Journal SU. 4(1), 891-901.
Punsuvon, V., Anpanurak, W., Vaithanomsat, P. & Tungkananuruk, N. (2005). Fraction of Chemical Components of Oil Palm Trunk by Steam Explosion. 31st Congress on Science and Technology of Thailand at Suranaree University of Technology.18-20 October 2005.
Punsuvon, V., (Eds) Chemical Component Determination of Non Wood Raw Material for Pulp and Paper Industry, Final Report, The Research Project for Higher Utilization of Forestry and Agricultural Plant Materials in Thailand (HUFA)
Subkaree, Y. 2008. Saccharification and fermentation (SSF) optimal condition for ethanol production from palm pressed fiber using simultaneous saccharification and fermentation (SSF). Master Thesis, Prince of Songkla University, (In Thai)
Sugiharto, Y. E. C., Harimawan, A., Kresnowati, M.T.A.P, Purwadi, R., Mariyana, R, Andry, Fitriana, H.N., & Hosen, H. F. (2016). Enzyme feeding strategies for better fed-batch enzymatic hydrolysis of empty fruit bunch. Bioresource technology. 207: 175-179.
Suwan. S. (N.D.). Oil Palm.Energy. Chooose. Retrieved January 20, 2015, from https://www2.moac.go.th/ewt_ dl_link.php?nid=6694
