Evaluation of Antioxidant Activity and Phytochemical from Kamkung (Chara corallina Klein ex C.L.Willnenow)
The objective of this research was to compare the phytochemical contents (total phenolic content, total flavonoid content, tannin content) and the potential of antioxidant activities, DPPH scavenging activity, ABTS radical scavenging activity and metal chelating activity of Chara corallina extract using 3 different extraction solvent (ethyl acetate, ethanol and methanol). The results showed all group of phytochemical and all method of antioxidant activities were significantly different among different solvent extract (p < 0.05). The ethyl acetate extract showed the highest of total phenolic compound and tannin content were 36.09±0.55 and 31.91±0.67 mgGAE/g extract, respectively and the highest activity of scavenging DPPH and metal chelation (IC50 = 0.169±0.01 and 0.098±0.00 mg/ml). The ethanolic extract showed the highest of total flavonoid contents was 23.11±0.04 mgQE/g extract and the best activity of scavenging ABTS with IC50 was 1.116±0.07 mg/mL. According to Pearson’s coefficient correlation between the extract with IC50 of antioxidant activities and the phytochemical contents, the results revealed that the total phenolic content and tannin content were major factors to stop antioxidant activities in all 3 study above. These findings clearly indicated that C. corallina had a significant potential to be used as natural antioxidants and to be develop into health products.
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