Chemical Sterilization in MS Culture Medium for In vitro Culture of Philodendron sp. “Ruaysap”

  • Raheema Wamaedeesa Faculty of Agriculture, Princess of Naradhiwas University, Meuang, Narathiwat, 96000
  • Bakren Ali
  • Nusanisa Chedao
  • Supanath Kanjanawattanawong
Keywords: Chemical sterilization, Haiter®, Betadine®, Philodendron, Tissue culture

Abstract

This study aimed to investigate the appropriate chemicals and concentrations for disinfection of medium used to culture Philodendron “Ruaysap” shoot tip. The shoot tips were cultured on MS medium sterilized by adding Haiter® and Betadine® at 2, 4 and 6 mL/L for 60 days. The results showed both chemicals with all concentrations were able to inhibit the growth of microorganisms. Besides, it was found that the medium sterilized by adding 2 mL/L Haiter® increased foliar growth with the maximum number, length and width of leaves (6.3 leaves, 1.39 cm, 0.82 cm, respectively). Medium sterilized by adding 2, 4 and 6 mL/L Betadine® resulted in no difference in root number and length but the addition of Betadine® at all concentrations gave the number of roots greater than those sterilized by both autoclaving and adding Haiter®. After acclimatization for 30 days in greenhouse, all complete plantlets survived and grew well. Therefore, Haiter® and Betadine® are able to replace autoclaving which is a costly method.

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Published
2021-01-13
How to Cite
Wamaedeesa, R., Ali, B., Chedao, N., & Kanjanawattanawong, S. (2021). Chemical Sterilization in MS Culture Medium for In vitro Culture of Philodendron sp. “Ruaysap”. Princess of Naradhiwas University Journal, 13(1), 377-387. Retrieved from https://li01.tci-thaijo.org/index.php/pnujr/article/view/246876
Section
บทความวิจัย