Inhibitory Effects of Stink Bean (Parkia speciose) Extracts Against α-amylase and α-Glucosidase Activity
Keywords:
α-amylase, α-glucosidase, Parkia speciosa (Sator) extract, enzyme inhibitionAbstract
Sator (Parkia speciosa Hassk) is the favorite plant in the 3 southern border provinces of Thailand. The sator extracts indicated the presence of antioxidants, antimicrobials and inhibition of cancer cells. However, the previous study of inhibition of α-amylase and α-glucosidase activity has not been reported. Both enzymes play an importance role of decreasing blood glucose levels resulting in amelioration of non-communication chronic diseases (NCDs). Therefore, this study focused on 4 distinct extraction methods. The results showed that the 50% ethanol extraction is the most ability to inhibit the α-amylase at 96.65 ± 6.58 % and IC50 at 0.42 mg/ml. The fifty percentage of ethanol extraction is a better inhibitor than acarbose for the α-amylase (p < 0.05). To investigate the preference of the α-glucosidase from intestinal rat inhibition, these extracts had less α-glucosidase inhibitory activity. The result of α-glucosidase from Saccharomyces cerevisiae was found to be most inhibition by 100 % ethanol extraction of 27.67 ± 2.38 % at the enzyme concentration of 0.25 mg/ml compared with the acarbose significantly (p < 0.05). The sator extracts have been able to inhibit both α-amylase and α-glucosidase activity. The data from this study can be used as supporting knowledge for further research in supplemental food and complementary medicine including prevention of NCDs.
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