Acaricidal Properties of Cuscuta spp. Crude Extracts and Bacteria against Luciaphorus perniciosu

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Keerati Tanruean
Suphak Kondara
Ruangwut Chutima
Tiwtawat Napiroon
Pisit Poolprasert

Abstract

The pyemotid mite (Luciaphorus perniciosus Rack, 1983) associated with several commercial mushrooms in Thailand that could result in the decline of mushroom cultivation. Therefore, the bioefficacies of Cuscuta reflexa and C. chinensis crude extracts and bacteria isolated from mushroom for controlling L. perniciosus infesting Jew’s ear mushroom (Auricularia auricular (Hook.) Underw.) were assessed in this study. Different concentrations of Cuscuta spp. crude extracts (0, 0.5, 1.0, 2.5 and 5% w/v) and the solution of each bacterium (1×108 CFU/mL) were prepared. The contact bioassay was designed in a completely randomized triplicate. A total of ten pregnant females of L. perniciosus were tested in each replication and concentration containing a sorghum seed inoculated with mycelia as food. After 3-, 5-, 7- and 9-day exposure, it was found that C. reflexa stem ethanolic extract exhibited median lethal concentrations to kill 50% (LC50) of the treated mites in 3, 5, 7 and 9 days of 22.86, 12.25, 5.87 and 2.01% w/v, respectively. Meanwhile, C. chinensis stem ethanolic extract displayed LC50 of 16.59, 7.34, 2.18 and 1.78% w/v, respectively. In terms of the mortality rate, the concentration at 5% w/v in both crude plant extracts showed effectiveness in killing pregnant mites with 100% mortality after exposure for 7 days (C. chinensis) and 9 days (C. reflexa). Conversely, the solution of all isolated bacteria could not kill pyemotid at this time. Nevertheless, these results suggested that both plant extracts are likely to be further applied as a biopesticide in preventing L. perniciosus and other mite pests of mushroom devastating the mushroom farm.

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How to Cite
Tanruean, K., Kondara, S., Chutima, R., Napiroon, T., & Poolprasert, P. (2021). Acaricidal Properties of Cuscuta spp. Crude Extracts and Bacteria against Luciaphorus perniciosu. Rajamangala University of Technology Srivijaya Research Journal, 13(3), 609-622. Retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/241849
Section
Research Article
Author Biographies

Keerati Tanruean, Faculty of Science and Technology, Pibulsongkram Rajabhat University

Biology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand.

Suphak Kondara, Faculty of Science and Technology, Pibulsongkram Rajabhat University

Biology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand.

Ruangwut Chutima, Faculty of Science and Technology, Pibulsongkram Rajabhat University

Biology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand.

Tiwtawat Napiroon, Faculty of Science and Technology, Thammasat University

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, Thailand.

Pisit Poolprasert, Faculty of Science and Technology, Pibulsongkram Rajabhat University

Biology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand.

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