Study of Morphology, Mycelial Growth and Exopolysaccharides Production of Cordyceps sp. Selected from Several Farms in Central Region of Thailand
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Abstract
Cordyceps militaris is an entomopathogenic fungus that can produce high levels of bioactive compounds such as cordycepin, adenosine, and polysaccharides. This research aimed to study mycelial morphology, growth, biomass, and exopolysaccharides production of Cordyceps sp. strains selected from several farms in central Thailand. Eleven samples of Cordyceps sp. were selected from six farms in Thailand. They were isolates CM-Sp01, CM-Sp02, CM-Sp03, CM-Sp04, CM-Sp05, CM-Sp06, CM-Sp07, CM-Sp08, CM-Sp09, CM-Sp10 and CM-Sp11. The hyphae morphology using the slide culture technique and microscopic examination were investigated. The results revealed that all isolates had septate hyphae. A round and an oval shape of conidia at the end of hyphae were also found. However, the size of conidia was different between Cordyceps sp. isolates. In addition, the study of morphology and density of mycelial colony of C. militaris isolates on PDA medium were determined. The results found that vegetative and aerial mycelium in all isolates were white. The isolate CM-Sp02 showed the highest mycelium density. Under determining the growth of mycelial colony by measuring colony diameter on PDA medium, isolate CM-Sp08 showed the highest growth rate (5.06 ± 0.65 mm/day) and length of diameter of mycelial colony (66.12 ± 1.45 mm) after 21 days of cultivation in PDA medium under temperature of 22 oC in dark condition. Mycelial biomass and exopolysaccharides in PDB medium were investigated. The result found that isolate CM-Sp08 showed the highest biomass production (55.20 ± 1.65 g/L) and the highest exopolysaccharide content (55.20 ± 1.65 g/L). This indicated that the isolate CM-Sp08 had high potential for growth, biomass production, and exopolysaccharide production.
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