Growth Improvement of a Marine Diatom Amphora subtropica BUUC1502 by Adding Phosphate and Silicate during the Cultivation
Keywords:
nutrient addition, phosphate, silicate, marine diatom, Amphora subtropica BUUC1502Abstract
Nitrate, phosphate, and silicate are important nutrients in the culture medium for diatom cultivation. This study investigated the growth of a marine diatom Amphora subtropica BUUC1502 cultured with an addition of nutrients alongside the culture period. The first experiment was to evaluate the growth and nutrient consumption of the diatom when cultured in the standard F/2 medium at 30 PSU salinity. The results showed that a decrease in nitrate concentration was found but the total concentration was still sufficient for the diatom cultivation (>8 mg-N/L). On the other hand, phosphate and silicate were substantially decreased to almost zero within 5 days. The second experiment was to investigate the effect of an addition of nitrate and phosphate during cultivation on the growth of diatom by cultivation of diatom in 6 different treatments including 1) standard F/2 medium, 2) F/2 medium without phosphate (F/2-P), 3) F/2 medium without silicate (F/2-Si), 4) F/2 medium with phosphate addition alongside the culture (F/2+P), 5) F/2 medium with silicate addition alongside the culture (F/2+Si), and 6) F/2 medium with phosphate and silicate addition alongside the culture (F/2+P+Si). The nutrient addition was performed during days 2-13 and the nutrient concentration was increased between days 14-19 to compensate higher cells density. It was found that an addition of both phosphate and silicate alongside the cultivation could enhance the growth of the diatom with the highest density of 609.44x104 cells/ml whereas culturing with F/2 medium alone resulted in a maximum cell density of 318.33x104 cells/ml. However, an increment in nutrient addition during the final period (days 14-19) did not provide further growth of the diatom.
References
Alipanah, L., Winge, P., Rohloff, J., Najafi, J., Brembu, T. and Bones, A.M. 2018. Molecular adaptations to phosphorus deprivation and comparison with nitrogen deprivation responses in the diatom Phaeodactylum tricornutum. PloS one 13(2): e0193335.
APHA. 1992. Standard for examination of water and wastewater. Victor Graphics, Maryland.
Bozarth, A., Maier, U.G. and Zauner, S. 2009. Diatoms in biotechnology: modern tools and applications. Applied Microbiology and Biotechnology 82(2): 195-201.
Brembu, T., Mühlroth, A., Alipanah, L. and Bones, A.M. 2017. The effects of phosphorus limitation on carbon metabolism in diatoms. Philosophical Transactions of the Royal Society B: Biological Sciences 372(1728): 20160406.
Chenchankhan, P. 2015. Isolation of culturable marine microalgae from aquaculture ponds, Chanthaburi province. Bachelor of science, Burapha University. (in Thai)
Frigolet, M.E. and Gutiérrez-Aguilar, R. 2017. The role of the novel lipokine palmitoleic acid in health and disease. Advances in Nutrition 8(1): 173S-181S.
Govindan, N., Maniam, G.P., Sulaiman, A.Z., Ajit, A., Chatsungnoen, T. and Chisti, Y. 2021. Production of renewable lipids by the diatom Amphora copulate. Fermentation 7(1): 37.
Grover, J.P. 1989. Effects of Si: P supply ratio, supply variability, and selective grazing in the plankton: An experiment with a natural algal and protistan assemblage. Limnology and Oceanography 34(2): 349-367.
Guillard, R.R., Kilham, P. and Jackson, T.A. 1973. Kinetics of silicon limited growth in the marine diatom Thalassiosira pseudonana hasle and heimdal (= Cyclotella nana Hustedt) 1, 2. Journal of Phycology 9(3): 233-237.
Guillard, R.R. and Sieracki, M.S. 2005. Counting cells in cultures with the light microscope, pp. 239-252. In Andersen, R.A., eds. Algal culturing techniques. Elsevier, UK.
Hogan, P., Otero, P., Murray, P. and Saha, S.K. 2021. Effect of biomass pre-treatment on supercritical CO2 extraction of lipids from marine diatom Amphora sp. and its biomass evaluation as bioethanol feedstock. Heliyon 7(1): e05995.
Khatoon, H., Banerjee, S., Yusoff, F. and Shariff, M. 2009. Evaluation of indigenous marine periphytic Amphora, Navicula and Cymbella grown on substrate as feed supplement in Penaeus monodon postlarval hatchery system. Aquaculture Nutrition 15(2): 186-193.
Kumaran, J., Jose, B., Joseph, V. and Bright Singh, I.S. 2017. Optimization of growth requirements of marine diatom Chaetoceros muelleri using Response Surface Methodology. Aquaculture research 48(4): 1513-1524.
Kutako, M., Khamtongdee, S., Setthamongkol, P., Jamkratoke, M. and Yuvanatemiya, V. 2014. The effect of nutrients on growth of a marine diatom, Chaetoceros sp., in recycled diatom cultured water. Journal of Fisheries Technology Research 8(1): 74-83. (in Thai)
Lebeau, T. and Robert, J.M. 2003. Diatom cultivation and biotechnologically relevant products. Part I: Cultivation at various scales. Applied Microbiology and Biotechnology 60(6): 612-623.
Lee, R.E. 1999. Phycology. Cambridge University Press, Cambridge.
Li, X.L., Marella, T.K., Tao, L., Li, R., Tiwari, A. and Li, G. 2017. Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates. Phycological Research 65(3): 177-187.
Lippemeier, S., Hartig, P. and Colijn, F. 1999. Direct impact of silicate on the photosynthetic performance of the diatom Thalassiosira weissflogii assessed by on-and off-line PAM fluorescence measurements. Journal of Plankton Research 21(2): 269-283.
Martin-Jézéquel, V., Hildebrand, M. and Brzezinski, M.A. 2000. Silicon metabolism in diatoms: implications for growth. Journal of Phycology 36(5): 821-840.
Pan, Y., DV, S. R., Mann, K., Brown, R. and Pocklington, R. 1996. Effects of silicate limitation on production of domoic acid, a neurotoxin, by the diatom Pseudo-nitzschia multiseries. I. Batch culture studies. Marine Ecology Progress Series 131: 225-233.
Parsons, T.R., Maita, Y. and Lalli, C.M. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press, Oxford.
Prasopwong, A., Nootong, K. and Kutako, M. 2020. Effect of water from shrimp pond on growth and fucoxanthin production of a diatom Amphora subtropica BUUC1502. Khon Kaen Agriculture Journal 48(1): 123-128. (in Thai)
R Core Team. 2020. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
Sargent, J., McEvoy, L., Estevez, A., Bell, G., Bell, M., Henderson, J. and Tocher, D. 1999. Lipid nutrition of marine fish during early development: current status and future directions. Aquaculture 179(1-4): 217-229.
Scala, S. and Bowler, C. 2001. Molecular insights into the novel aspects of diatom biology. Cellular and Molecular Life Sciences CMLS 58(11): 1666-1673.
Strickland, J.D.H. and Parsons, T.R. 1972. A Practical Handbook of Seawater Analysis. Fisheries Research Board of Canada Bulletin, Ottawa.
Tiwari, A., Melchor-Martínez, E.M., Saxena, A., Kapoor, N., Singh, K.J., Saldarriaga-Hernández, S., Iqbal, H.M. 2021. Therapeutic attributes and applied aspects of biological macromolecules (polypeptides, fucoxanthin, sterols, fatty acids, polysaccharides, and polyphenols)from diatoms-A review. International Journal of Biological Macromolecules 171: 398-413.
Trainor, F.R. 1978. Introductory Phycology. John Wiley and Sons, New York.
Tapaneeyaworawong, P., Pantong, P., Chumtong, P., Kutako, M., Hiransuchalert, R., Paibulkichakul, C. and Powtongsook, S. 2018. Batch and continuous cultures of marine benthic diatom Amphora subtropica BUUC1502 isolated from Pacific white shrimp pond. Walailak Procedia 2(13): 1-8. (in Thai)
Viriyayingsiri, T. 2009. Optimal conditions for diatom Entomoneis sp. cultivation in batch and continuous cultures. Master’s thesis, Chulalongkorn University. (in Thai)
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