The Use of Synthetic Hormone Buserelin Acetate (BUS) in Combination with the Synergistic Domperidone (DOM) to Induce the Spawning of Lambchop Rasbora (Trigonostigma espei)

Abstract

The administration of the synthetic hormone buserelin acetate (BUS) in combination with the synergistic domperidone (DOM) to induce the spawning of Lambchop rasbora (Trigonostigma espei) was studied using the methods of in-feed medication, gavage, and intramuscular injection. Fish were bred in a glass tank imitating nature with the broodstock ratios of 3:3 fish and 1:1 fish. It revealed that Lambchop rasbora did not spawn after providing BUS in combination with DOM through the methods of in-feed medication and gavage. However, the breeding of this fish species could be carried out via intramuscular injection, with male fish receiving combinations of BUS 10 µg/kg and DOM 10 mg/kg, and female fish receiving combinations of BUS 15 µg/kg and DOM 10 mg/kg. The induced fish had spawned approximately 10 hours after injection and the fry began to hatch approximately 19 hours later. Fish breeding with broodstock ratios of 3:3 fish and 1:1 fish showed similar results in the evaluated parameters. It indicated that the average numbers of eggs were 50.25+13.45 and 55.50+6.61, the average fertilization rates were 81.25+1.69 and 81.12+1.34 percent, the average hatching rates were 79.40+1.56 and 79.45+1.24 percent, the average numbers of fry after hatching for 3 days were 19.25+4.65 and 21.75+2.63, and the survival rates of fry were 59.73+1.20 and 60.81+1.79 percent, respectively. Moreover, only a couple of fish mated in all tanks containing the breeder ratio of 3:3. There was no mating in the sex ratios of 1:2 fish and 1:3 fish when considering mainly both the male and female breeders. Therefore, the suitable sex ratio of the fish breeder was 1:1.