Effect of Sodium Hypochlorite on Sterilization and Shoot Induction of In Vitro Gros Michel
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Abstract
Musa acuminata cv. Gros Michel is originated from the Southeast Asia region. It is considered as an economically important fruit tree in Thailand. The suckers are commonly used for propagation and plant tissue culture for increasing new pathogen-free plants and genetic uniformity. This research was divided into 2 experiments. The first experiment investigated the effectiveness of different concentrations of sodium hypochlorite on surface sterilization of sucker including treatment 1 10 % NaOCl followed by 5 %, treatment 2 20 % NaOCl followed by 10 % and treatment 3 30 % NaOCl followed by 20 %. The second experiment examined 4 concentration levels of BA at 0, 2.5, 5.0, and 7.5 mg/l. The results revealed that NaOCI at 30% followed by 20% showed no statistically significant difference in survival (100%) compared to NaOCI at 20% followed by 10% (91.67%) after one month of cultivation on MS medium. In experiment 2, at 0 mg/l BA showed the best growth in terms of plant height, leaf length, leaf width, and root length at 6.60, 5.82, 2.47, and 11.27 cm, respectively after three months of cultivation. However, multiple shoot formation was not obtained after culturing for 3 months. The culture medium with 5 mg/l BA could induce multiple shoots, with the observation of four new shoots after culturing for three months.
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