Most Pobable Number-Polymerase Chain Reaction-based Quantification of Escherichia coli O26, O104, O111, and O157 from Thai and Imported Malaysian Beef
Article Sidebar
Main Article Content
Abstract
The contamination of Escherichia coli O157 and non-O157 groups in foods has been reported to cause sporadic cases and outbreaks, resulting in significant economic and social losses worldwide. In this study, the prevalence of E. coli O26, O104, O111, and O157 in retail beef samples from either domestic Thai or imported Malaysian sources was investigated and their amounts were quantified using the MPN-PCR approach. Of the 102 samples, E. coli O26, O104, O111, and O157, were found in beef at rates of 57%, 6%, 0%, and 17%, respectively, with serotype O26 showing the highest contamination rate among the sample (P-value < 0.05). Among the positive samples investigated, the lowest MPN/g value were less than 3 for all serotypes, while the highest MPN/g values of E. coli O26, O104, O111, and O157, were 75, 93, < 3, and 27, respectively. Immuno-magnetic separation technique was also employed to confirm the lack of E. coli O111 in beef. The high level of E. coli O157 and non-O157 contamination in beef observed in this study are thought to pose health risks to consumers, making the finding crucial from a public health standpoint.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The content and information in the article published in Journal of Rajamangala University of Technology Srivijaya It is the opinion and responsibility of the author of the article. The editorial journals do not need to agree. Or share any responsibility.
References
Bielaszewska, M., Mellmann, A., Zhang, W., Köck, R., Fruth, A., Bauwens, A., Peters, G. and Karch, H. 2011. Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infectious Diseases 11(9): 671-676.
Brooks, J.T., Sowers, E.G., Wells, J.G., Greene, K.D., Griffin, P.M., Hoekstra, R.M. and Strockbine, N.A. 2005. Non-O157 Shiga-toxin-producing Escherichia coli infection in the United States, 1983-2002. Journal of Infectious Diseases 192(8): 1422-1429.
Brown, J.A., Hite, D.S., Gillim-Ross, L.A., Maguire, H.F., Bennett, J.K., Patterson, J.J., Comstock, N.A., Watkins, A.K., Ghosh, T.S. and Vogt, R.L. 2012. Outbreak of Shiga toxin-producing Escherichia coli serotype O26:H11 infection at a child care center in Colorado. Pediatric Infectious Disease Journal 31(4): 379-383.
Bugarel, M., Beutin, L., Scheutz, F., Loukiadis, E. and Fach, P. 2011. Identification of genetic markers for differentiation of Shiga toxin-producing, enteropathogenic, and avirulent strains of Escherichia coli O26. Applied and Environmental Microbiology 77(7): 2275-2281.
Chapman, P.A., Wright, D.J. and Siddons, C.A. 1994. A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from bovine faeces. Journal of Medical Microbiology 40(6): 424-427.
de Sablet, T., Bertin, Y., Vareille, M., Girardeau, J.P., Garrivier, A., Gobert, A.P. and Martin, C. 2008. Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C. Microbiology 154(Pt1): 176-186.
Durso, L.M., Bono, J.L. and Keen, J.E. 2005. Molecular serotyping of Escherichia coli O26:H11. Applied and Environmental Microbiology 71(8): 4941-4944.
Durso, L.M., Bono, J.L. and Keen, J.E. 2007. Molecular serotyping of Escherichia coli O111 :H8. Journal of Microbiological Methods 69(2): 381-383.
Ethelberg, S., Smith, B., Torpdahl, M., Lisby, M., Boel, J., Jensen, T., Nielsen, E.M. and Mølbak, K. 2009. Outbreak of non-O157 Shiga toxin-producing Escherichia coli infection from consumption of beef sausage. Clinical Infectious Disease 48(8): e78-81.
European Food Safety Authority (EFSA). 2007. Scientific opinion of the panel on biological hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic type. Available Source: http://www.efsa.europa.eu/en/efsajournal/doc/579.pdf, May 29, 2021.
Frank, C., Faber, M.S., Askar, M., Bernard, H., Fruth, A., Gilsdorf, A., Höhle, M., Karch, H., Krause, G., Prager, R., Spode, A., Stark, K. and Werber, D. on behalf of the HUS investigation team. 2011. Large and ongoing outbreak of haemolytic uraemic syndrome, Germany, May 2011. Euro surveillance 16(21): 19878.
Jeon, B.W., Jeong, J.M., Won, G.Y., Park, H., Eo, S.K., Kang, H.Y., Hur, J. and Lee, J.H. 2006. Prevalence and characteristics of Escherichia coli O26 and O111 from cattle in Korea. International Journal of Food Microbiology 110(2): 123-126.
Karch, H., Bielaszewska, M., Bitzan, M. and Schmidt, H. 1999. Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. Diagnostic Microbiology and Infectious Disease 34(3): 229-243.
Kayali, A.Y., Escalante-Maldonado, O., Vuddhakul, V., Seto, K., Nakaguchi, Y. and Nishibuchi, M. 2015. Development of a method for detection of Shiga toxin-producing Escherichia coli belonging to clinically important twelve O serotypes based on the combination of PickPen-assisted immunomagnetic separation and loop-mediated isothermal amplification. International Journal of Immunology and Immunotherapy 2(1): 1-7.
Liu, L., Johnson, H.L., Cousens, S., Perin, J., Scott, S., Lawn, J.E., Rudan, I., Campbell, H., Cibulskis, R., Li, M., Mathers, C. and Black, R.E. child health epidemiology reference group of WHO and UNICEF. 2012. Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000. Lancet 379(9832): 2151-2161.
Mäntynen, V., Niemelä, S., Kaijalainen, S., Pirhonen, T. and Lindström. K. 1997. MPN-PCR-quantification method for staphylococcal enterotoxin c1 gene from fresh cheese. International Journal of Food Microbiology 36(2-3): 135-143.
Maurer, J.J., Schmidt, D., Petrosko, P., Sanchez, S., Bolton, L. and Lee, M.D. 1999. Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Applied and Environmental Microbiology 65(7): 2954-2960.
Michino, H., Araki, K., Minami, S., Takaya, S., Sakai, N., Miyazaki, M., Ono, A. and Yanagawa H. 1999. Massive outbreak of Escherichia coli O157:H7 infection in schoolchildren in Sakai city, Japan, associated with consumption of white radish sprouts. American Journal of Epidemiology 150(8): 787-796.
Nataro, J.P. and Kaper, J.B. 1998. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews 11(1): 142-201.
Paton, A.W., Ratcliff, R.M., Doyle, R.M., Seymour-Murray, J., Davos, D., Lanser, J.A. and Paton, J.C. 1996. Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli. Journal of Clinical Microbiology 34(7): 1622-1627.
Phetkhajorn, S., Sirikaew, S., Rattanachuay, P. and Sukhumungoon, P. 2014. Most probable number-polymerase chain reaction-based quantification of enterotoxigenic Escherichia coli from raw meats in southern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 45(6): 1385-1391.
Picozzi, C., Foschino, R. and Di Pilato, P. 2004. Exploiting a MPN-PCR technique to quantify Escherichia coli in minced meat. Annals of Microbiology 54(3): 343-349.
Riley, L.W., Remis, R.S., Helgerson, S.D., McGee, H.B., Wells, J.G., Davis, B.R., Hebert, R.J., Olcott, E.S., Johnson, L.M., Hargrett, N.T., Blake, P.A. and Cohen, M.L. 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308(12): 681-685.
Sae-lim, A., Jearanai, P., Rattanachuay, P. and Sukhumungoon, P. 2017. Prevalence, virulence profiles, and genetic relationship of atypical enteropathogenic Escherichia coli O145 from beef, southern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 48(6): 1248-1259.
Sae-lim, A., Wameadesa, N., Rattanachuay, P. and Sukhumungoon, P. 2018. Prevalence, molecular characterization and genetic relatedness of Escherichia coli O103 from meat in southern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 49(4): 646-659.
Sukhumungoon, P., Nakaguchi, Y., Ingviya, N., Pradutkanchana, J., Iwade, Y., Seto, K., Radu, S., Nishibuchi, M. and Vuddhakul V. 2011. Investigation of stx2+ eae+ Escherichia coli O157:H7 in beef imported from Malaysia to Thailand. International Food Research Journal 18(1): 381-386.
Sirikaew, S., Rattanachuay, P., Nakaguchi, Y. and Sukhumungoon, P. 2015. Immuno-magnetic isolation, characterization and genetic relationship of Escherichia coli O26 from raw meats, Hat-Yai city, Songkhla, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 46(2): 241-253.
Sirikaew, S., Sukkua, K., Rattanachuay, P., Khianngam, S. and Sukhumungoon, P. 2016. High level of Shiga toxin-producing Escherichia coli and occurrence of stx-negative E. coli O157 from raw meats: characterization of virulence profile and genetic relatedness. Southeast Asian Journal of Tropical Medicine and Public Health 47(5): 1008-1019.
Themphachana, M., Nakaguchi, Y., Nishibuchi, M., Seto, K., Rattanachuay, P., Singkhamanan, K. and Sukhumungoon, P. 2014. First report in Thailand of a stx-negative Escherichia coli O157 from a patient with diarrhea, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 45(4): 881-889.
Wameadesa, N., Sae-lim, A., Hayeebilan, F., Rattanachuay, P. and Sukhumungoon, P. 2017. Enteroaggragative Escherichia coli O104 from Thai and imported Malaysian raw beef. Southeast Asian Journal of Tropical Medicine and Public Health 48(2): 338-350.