Feasibility of Using Durian Seeds, Jackfruit Seeds and Cassava as a Replacement for Rice in the Culture of Trichoderma harzianum in the Khao Khitchakut Community
Keywords:
Durian seeds, jackfruit seeds, cassava, rice seed, Trichoderma harzianumAbstract
Currently, in the Khao Kitchakut community of Chanthaburi province, the production volumes of durian, jackfruit and cassava have increased, resulting in a large amount of waste materials from the agricultural sector and the fruit product processing industry. Consequently, a significant amount of agricultural and fruit processing byproducts exists. In particular, there is a substantial quantity of discarded seeds which, if not properly managed, could become a breeding ground for various plant diseases like root rot, stem rot, pink disease, and fruit rot in durian. Therefore, maximizing the utilization of these remaining seed portions becomes imperative. The objective of this study is to investigate the feasibility of using durian seeds, jackfruit seeds, and cassava as alternatives to rice for cultivating Trichoderma harzianum. The experimental design employs a Completely Randomized Design (CRD) with four treatments, with ten repetitions: Treatment 1 – rice seeds; Treatment 2 – durian seeds; Treatment 3 – jackfruit seeds; and Treatment 4 – cassava.The rice method showed the highest mycelial growth and sporulation, with 100% colonization and 1.66x106 spores per milliliter, respectively. Cassava and jackfruit seeds exhibited the lowest mycelial growth and sporulation, while durian seeds showed only 20% colonization and 0.5x104 spores per milliliter, respectively. This was due to contamination by Aspergillus sp. and Penicillium sp., resulting in the lowest mycelial growth and sporulation of Trichoderma sp. Therefore, we conclude that using durian and jackfruit seeds as substitutes for rice to cultivate Trichoderma sp. is not suitable for the propagation of the fungus.
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