Artificial Seawater Production for Pacific White Leg Shrimp (Litopenaeus vannamei) Culture in Closed System

Authors

  • Chuthamat Thaklaewphan Department of Aquaculture, Faculty of Agricultural Technology, Phetchaburi Rajabhat University, Muang, Phetchaburi 76000, Thailand.

Keywords:

artificial seawater, pacific white shrimp, sea salt

Abstract

Concentrated brine a by-product of Thai sea salt farming can be used for raising low-salinity marine shrimp in a closed system. However, transporting and storing brine remain challenging, and its mineral composition is unstable. This research aims to study the effect of different artificial seawater treatments, flowers of salt and artificial seawater prepared from concentrated salt water on the growth of white leg shrimp (Litopenaeus vannamei) in a closed system using a completely randomized design. The experiment included three treatments: “(1) a control unit using brine (280 ppt) from a Naklua farm, (2) flower of salt obtained from the farm, and (3) artificial seawater prepared from 60 ppt. concentrated saltwater used to accelerate crystallization. Seventy-two shrimp with an initial average weight of 11.42±2.21 g were randomly placed into 200-liter fiber tanks at a density of 8 shrimp/m², with constant aeration for 60 days, while water salinity was maintained at 15 ppt. Monitoring parameters included shrimp weight, survival rate, molting frequency, and water quality. The results showed that weight gain and survival rates of shrimp across all treatments were not statistically different (p>0.05). Specifically, the survival rates for treatments 1, 2, and 3 were 98.0%, 97.3%, and 97.5%, respectively. However, FCR values in treatments 2 and 3 were lower than in treatment 1. (p<0.05)  The color of boiled shrimp was compared using the Munsell Color Book, resulting in 5YR 5/12, 5YR 7/6, and 5YR 6/8 , respectively. The result from the sensory evaluation of appearance, odor, taste, and texture of shrimp showed that the three artificial seawater formulations received scores ranging from 6.47 to 6.77 (“like moderately”). Therefore, artificial seawater produced from brine and flowers of salt can be used, as the growth, survival rate, and sensory evaluation were not significantly different from those of conventional saline cultures.

References

Armenta-Bojórquez, A.D., Valenzuela-Castañeda, A.R., Fitzsimmons, K., López-Alvarez, E.S., Rodríguez-Quiroz, G. and Valenzuela-Quiñónez, W. 2021. Pacific white shrimp and tomato production using water effluents and salinity-tolerant grafted plants in an integrated aquaponic production system. Journal of Cleaner Production 278: 124064.

Boyd, C.A., Boyd, C.E. and Rouse, D.B. 2007. Potassium budget for inland, saline water shrimp ponds in Alabama. Aquacultural Engineering 36(1): 45-50.

Charenphak, V., Chanaphai, B., Reuangthong, J., Prairarin, K. and Ong-Art, K. 2020. Study on Pacific white shrimp (Litopenaeus vannamei Boon, 1931) for brood stock breeding program. E-thai fisheries gazette 3(4): 49-67. (in Thai)

Citarasu, T., Babu, M.M. and Yilmaz, E. 2022. Alternative medications in shrimp health management for improved production. Aquaculture 561: 738695.

Keawla-iad, N. and Pratoomchat, B. 2010. Proportional supplementation of minerals in cultured medium on growth, survival, food conversion ratio, molt frequency and size variation of white shrimp (Litopenaeus vannamei), pp. 83-91. In 48th Kasetsart University Annual Conference: Fisheries. Kasetsart University, Bangkok. (in Thai)

Laisutisan, K., Limsuwan, C., Wongmaneeprateep, S., Prasertsri, S., Limhang, K., Wiriyapattanasub, P. and Sitthimonton, S. 2010. Effect of temperature during postlarval rearing of Litopenaeus vannamei on growth and survival rate, pp. 322-328. In 48th Kasetsart University Annual Conference: Fisheries. Kasetsart University, Bangkok. (in Thai)

Lin, Y.C. and Chen, J.C. 2001. Acute toxicity of ammonia on Litopenaeus vannamei Boone juveniles at different salinity levels. Journal of Experimental Marine Biology and Ecology 259(1): 109-119.

Lin, Y.C. and Chen, J.C. 2003. Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels. Aquaculture 224(1-4): 193-201.

Lin, Y., Jing, S., Lee, D., Chang, Y., Chen, Y. and Shih, K. 2005. Performance of a constructed wetland treating intensive shrimp aquaculture wastewater under high hydraulic loading rate. Environmental Pollution 134(3): 411-421.

Moura, P., Neto, I.A., Brandão, H., Furtado, P., Poersch, L., Wasielesky, J.W. 2023. Effects of magnesium reduction in artificial low-salinity water on the growth of Pacific white shrimp Litopenaeus vannamei in a biofloc system. Aquaculture 577: 739956.

Nehru, E., Chandrasekhara Rao, A., Pamanna, D. and Priyadarshini, N., Anil Kumar, P. and Raveendra, M. 2018. Effect of dietary minerals supplementation on growth and survival of Litopenaeus vannamei in low salinity water. International Journal of Current Microbiology and Applied Sciences 7(3): 3040-3049.

Pan, L.Q., Luan, Z.H. and Jin, C.X. 2006. Effects of Na+/K+ and Mg+ /Ca+ ratios in saline ground waters on Na+ –K+ -ATPase activity, survival and growth of Marsupenaeus japonicus postlarvae. Aquaculture 261: 1396-1402.

Pratoomchat, B. and Sommart, S. 2000. Effect of salinity on biomineralization of White Leg Shrimp (Litopenaeus vannamei), pp. 73-82. In Kasetsart University Annual Conference: Fisheries 48th. Kasetsart University, Bangkok. (in Thai)

Pratoomchat, B., Hongsapruek, K. and Keawla-iad, N. 2011. Research Report on Proportional supplementaion of minerals in cultured medium on physicochemical of Litopenaeus vannamei shrimp. Burapha University, Chonburi. (in Thai)

Roy, L.A., Davis, D.A., Saoud, I.P. and Henry, R.P. 2007. Effects of varying levels of aqueous potassium and magnesium on survival, growth, and respiration of the Pacific white shrimp, Litopenaeus vannamei, reared in low salinity waters. Aquaculture 262: 461-469.

Serrano, L., Towle, D.W., Charmantier, G. and Spanings-Pierrot, C. 2007. Expression of Na +/K +-ATPase α-subunit mRNA during embryonic development of the crayfish Astacus leptodactylus. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2(2): 126-134.

Sirithanawong, B. and Thakleawphan, C. 2019. The use of dried sea water and epsom salt from the wisdom of Thai sea salt farming for of White Leg Shrimp (Litopenaeus vannamei) culture and Giant freshwater prawn (Macrobrachium rosenbergii) rearing. Available Source: http://research.pbru.ac.th/researchexpo2017/docs/salt/1_1_salt.pdf, May 1, 2022. (in Thai)

Sommart, S. 2009. Biomineralization of Litopenaeus vannamei and application of minerals supplementation in commercial larviculture and grow-out systems. Master of Science (Aquatic Science), Burapha University. (in Thai)

Suphawinyoo, K., Savangarrom, Y. and Kaewchada, N. 2013. Research Report on Addition of potassium magnesium in Giant freshwater prawn (Macrobrachium rosenbergii) in water reduce. Maejo university, Chumphon. (in Thai)

Thammanon, W. and Aussavamas, D. 2012. Archaeometric study of ancient pottery from Ban Dee Kilns, Pattani province. Prince of Songkhla University Pattani Campus research report; Pattani. Available Source: https://soreda.oas.psu.ac.th/show_detail.php?research_id=944, April 1, 2022. (in Thai)

Villarreal, H., Hinojosa, P. and Naranjo, J. 1994. Effect of temperature and salinity on the oxygen consumption of laboratory produced Penaeus vannamei postlarvae. Comparative Biochemistry and Physiology 108A: 331-336.

Wang, W.N., Wangb, A.L., Wanga, D.M., Wanga, L., Liua, Y. and Sunb, R.Y. 2003. Calcium, phosphorus and adenylate levels and Na+ –K+ -ATPase activities of prawn, Macrobrachium nipponense, during the moult cycle. Comparative Biochemistry and Physiology 134: 297-305.

Wudtisin, I. and Boyd, C.E. 2011. Possible potassium and magnesium limitations for shrimp survival and production in low-salinity, pond waters in Thailand. Journal of the World Aquaculture Society 42(6): 766-777.

Zhu, C., Dong, S., Wang, F. and Huang, G. 2004. Effects of Na/K ratio in seawater on growth and energy budget of juvenile Litopenaeus vannamei. Aquaculture 234(1-4): 485-496.

SEM images and XRD patterns of seawater crystals synthesized from brine 280 ppt in treatment 1

Published

2025-04-30

How to Cite

Thaklaewphan, C. (2025). Artificial Seawater Production for Pacific White Leg Shrimp (Litopenaeus vannamei) Culture in Closed System. Recent Science and Technology, 17(2), 259815. retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/259815

Issue

Section

Research Article