Nutrient Quality and Digestibility Improvement of Suwan Corn Ensiled with Microorganisms by in vitro Technique
Keywords:
corn silage, Bacillus subtillis, microbial activator super LDD2, silage quality, in vitro techniqueAbstract
Inoculation of Bacillus subtillis (BS) in corn silage improves the quality of the silage. Moreover, the microbial activator super LDD2 (LDD2) contains microorganisms that digest fiber and can produce lactic acid. Therefore, the objective is to use BS and LDD2 to ensile with corn to improve the quality of silage and its digestibility in the rumen. The experiment was designed as a 3 × 4 factorial, completely randomized design. Three Suwan corn varieties were used: 4452, 5731, and 5819 (factor 1) along with four groups of microorganism inoculations: whole plant corn, corn silage, corn silage ensiled with BS and corn silage ensiled with LDD2 (factor 2). The results showed that the corn varieties did not affect the quality and digestibility of corn silage (P>0.05), but did affect neutral detergent fiber and soluble carbohydrates (P<0.05). The interaction between corn varieties and microbial inoculation affected crude protein in corn silage (P<0.05). Inoculation of Bacillus subtilis and microbial activator super LDD2 resulted in an increase in crude protein (P<0.05). In addition, the digestibility of dry matter, crude protein, and neutral detergent fiber in the microbial inoculation treatments was better than the control corn silage (P<0.05). These treatments also resulted in a higher total bacterial count (P<0.05) and increased total volatile fatty acids in the rumen (P<0.05). Therefore, ensiling Suwan corn with BS and LDD2 can increase the quality of corn silage, affecting its nutritional value and digestibility, and can be considered an alternative option for improving roughage quality.
References
Aekatasanawan, C., Aekatasanawan, C., Changsaluk, S., Chaiwong, U. and Thala, P. 2015. Grain and Seed Production Technology of Single Cross Hybrid Suwan 4452 breed. National Corn and Sorghum Research Center. Available Source: https://www.thai-explore.net/file_upload/submitter/file_doc/c33ce970f2be31209818e46c611fc55e.pdf, January 25, 2024. (in Thai)
AOAC. 1990. Official Methods of Analysis of the Association of Official Analytical Chemistry (15thed). AOAC., Washington, D.C.
Bai, S.P., Wu, A.M., Ding, X.M., Lei, Y., Bai, J., Zhang, K.Y. and Chio, J.S. 2013. Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poultry science 92(3): 663-670.
Chang, M., Ma, F., Wei, J., Liu, J., Nan, X. and Sun, P. 2021. Live Bacillus subtilis natto promotes rumen fermentation by modulating rumen microbiota in vitro. Animals (Basel) 11(6): 1519.
Daşkıran, M., Önol, A.G., Cengiz, Ö., Ünsal, H., Türkyılmaz, S., Tatlı, O. and Sevim, Ö. 2012. Influence of dietary probiotic inclusion on growth performance, blood parameters, and intestinal microflora of male broiler chickens exposed to posthatch holding time. Journal of Applied Poultry Research 21(3): 612-622.
Davis, M.E., Parrott, T., Brown, D.C., De Rodas, B.Z., Johnson, Z.B., Maxwell, C.V. and Rehberger, T. 2008. Effect of a Bacillus-based direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs. Journal of animal science 86(6): 1459-1467.
Department of Livestock Development. 2001. Silage. Agricultural Cooperatives Association of Thailand Printing Company Limited, Bangkok.
Department of Land Development. 2023. Production of Bio-Fermented solution by Microbial Activator PD.2. Department of Land Development. Available Source: https://www.ldd.Source:nu_5wonder/PDF/PD2.pdf, March 20, 2023. (in Thai)
Elferink, S.J.W.H.O., Driehuis, F., Gottschal, J.C. and Spoelstra, S.F. 2000. Silage fermentation processes and their manipulation. FAO Plant Production and Protection Papers.
Fugita, C.A., Prado, I.N.D., Jobim, C.C., Zawadzki, F., Valero, M.V., Pires, M.C.D.O. and Françozo, M.C. 2012. Corn silage with and without enzyme-bacteria inoculants on performance, carcass characteristics and meat quality in feedlot finished crossbred bulls. Revista Brasileira de Zootecnia 41: 154-163.
Fuller, R. 1989. Probiotics in man and animals. Journal of Applied Bacteriology 66: 365-378.
Goering, H.K. and Van Soest, P.J. 1970. Forage fiber analyses. Agri Handbook, Washington.
Grubb, J. and Dehority B. 1976. Variation in colony counts of total viable anaerobic rumen bacteria as influenced by media and cultural methods. Applied and Environmental Microbiology 31(2): 262-267.
Guan, H., Shuai, Y., Yan, Y., Ran, Q., Wang, X., Li, D. and Zhang, X. 2020. Microbial community and fermentation dynamics of corn silage prepared with heat-resistant lactic acid bacteria in a hot environment. Microorganisms 8(5): 719.
Lounglawan, P. and Suksombat, W. 2015. Effect of Lactobacillus spp. on Silage Fermentation. Research reports, Suranaree University. Available Source: http://sutir.sut.ac.th:8080/sutir/bitstream/123456789/5809/1/Fulltext.pdf, January 25, 2024. (in Thai)
Panichpol, V., Phaikaew, C., Phromma, S., Chinvaroj, S., Arananant, J. and Ritreuchai, V. 2004. Silage Standard (1sted). Department of Livestock Development, Agricultural Cooperatives Association of Thailand Printing Company Limited, Bangkok. (in Thai)
Rahman, M.S., Mustari, A., Salauddin, M. and Rahman, M.M. 2013. Effects of probiotics and enzymes on growth performance and haematobiochemical parameters in broilers. Journal of the Bangladesh Agricultural University 11(1): 111-118.
Reid, G. 2008. Review: probiotics and prebiotics – progress and challenges. International Dairy Journal 18: 969-975.
SAS. 2022. SAS®University Edition software. In. SAS Institute Inc.
Seo, J.K., Kim, S.W., Kim, M.H., Upadhaya, S.D., Kam, D.K. and Ha, J.K. 2010. Direct-fed microbials for ruminant animals. Asian-Australasian Journal of Animal Sciences 23(12): 1657-1667.
Tassone, S., Fortina, R. and Peiretti, P.G. 2020. In vitro techniques using the DaisyII incubator for the assessment of digestibility : A review. Animals 10(5): 775.
Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583-3597
Vieira, V.A., Sforcini, M.P., Endo, V., Magioni, G.C. and Oliveira, M.D.S. 2014. Influence of probiotics on dairy cows’ diet. International Journal of Agricultural and Biological Engineering 8(7): 786-789.
Wang, M., Yang, C., Jia, L. and Yu, K. 2014. Effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation characteristics and aerobic stability of whip grass silage in laboratory silos. Japanese Journal of Grassland Science 60: 233-239.
Zhang, Q., Yu, Z. and Wang, X. 2015. Isolating and evaluating lactic acid bacteria strains with or without sucrose for effectiveness of silage fermentation. Grassland science 61(3): 167-176.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Recent Science and Technology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The content and information in the article published in Journal of Rajamangala University of Technology Srivijaya It is the opinion and responsibility of the author of the article. The editorial journals do not need to agree. Or share any responsibility.



