การใช้ประโยชน์ของสารสกัดหยาบเอนไซม์โบรมิเลนจากเศษเหลือทิ้งสับปะรด ต่อสมรรถภาพการผลิตแพะเนื้อ

wanida maksiri; Warinthorn Maneerat; Pitunart Noosen; Wisut Maitreejet; Samruay Malithod; Jenjina Tamraungit

Authors

wanida maksiri Department of Animal Science, Faculty of Agricultural Technology, Phetchaburi Rajabhat University, Mueng, Phetchaburi 76000, Thailand.
Warinthorn Maneerat School of Agriculture and Cooperatives, Sukhothai Thammathirat Open University, Pak Kret, Nonthaburi 11120, Thailand.
Pitunart Noosen School of Agriculture and Cooperatives, Sukhothai Thammathirat Open University, Pak Kret, Nonthaburi 11120, Thailand.
Wisut Maitreejet Department of Animal Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Samruay Malithod Department of Animal Science, Faculty of Agricultural Technology, Phetchaburi Rajabhat University, Mueng, Phetchaburi 76000, Thailand.
Jenjina Tamraungit Department of Animal Science, Faculty of Agricultural Technology, Phetchaburi Rajabhat University, Mueng, Phetchaburi 76000, Thailand.

Keywords:

meat goat, production performance, bromelain enzyme crude extract, pineapple waste

Abstract

The objective of this research was to study the effect of supplementing meat goats with crude bromelain enzyme extract from pineapple peels and crowns, mixed with Napier Pakchong 1 grass as roughage source on their production performance and rumen ecology. A total of nine male crossbred Native × Boer goats (average 5 months) with an initial body weight of 14.71±0.89 kg were used in the study. The experimental design was a completely randomized design (CRD) with three groups and three replication goats in each group. All goats were fed with fresh cut Napier Pakchong 1 grass (average age 60 days) as the main roughage source by ad libltum and supplemented with 2.5% (BW)concentrate feed (not less than 14 percent protein). Group 1 goats were fed with fresh Napier Pakchong 1 grass, group 2 and 3 goats were fed crude bromelain enzyme extract from pineapple peels and crowns mixed with Napier Pakchong 1 grass at ratio of 2:0.5 and 2:1 by fresh weight. The results showed that the use of crude bromelain enzyme extract from pineapple peels and crowns mixed with Napier Pakchong 1 grass at (2:0.5 and 2:1 ratio) led to a decrease in dry matter value and an increase in moisture and protein value in grass. However, it had no effect on dry matter intake, growth rate, blood chemical composition and the concentration of ammonia-nitrogen in the rumen fluid. In conclusion, the use of crude bromelain enzyme extract from pineapple peels and crowns mixed with Napier Pakchong 1 grass as roughage source did not improve the production performance of meat goats. However, there is a tendency to increase the quality of Napier Pakchong 1 grass with protein levels. Therefore, there should be further study on the use of crude bromelain enzyme extract from pineapple peels and crowns to improve the quality of Napier Pakchong 1 grass silage before applying it in goat farming.

References

AOAC. 2000. Official Method of Analysis of AOAC International (17th ed). The Association of Official Analytical Chemists, Virginia.

Bhattacharyya, B.K. 2008. Bromelain : An overview. Indian Journal of Natural Products and Resources 7(4): 359-363.

Cheva-Isarakul, B. 1998. Animal nutrition (6th ed). Thanaban Publication, Chiang Mai. (in Thai)

Ferguson, D.A., Li, C., Patel, N.R., Mayberry, W.R., Chi, D.S. and Thomas, E. 1993. Isolation of helicobacter pylori from saliva. Journal of Clinical Microbiology 31(10): 2802-2804.

Galyean, M. 1989. Procedure in Animal Nutrition Research. New Mexico: Department of Animal and Life Science, Mexico State University.

Hale, L.P., Greer P.K., Trinh C.T. and James C.L. 2005. Proteinase activity and stability of natural bromelain preparations. International Immunopharmacology 5(4): 783-793.

Hennessy, D.W. 1980. Protein nutrition of ruminants in the tropical areas of Australia. Tropical Grasslands 14: 260-265.

Henry, R.J., Donald, C.C. and James, W.W. 1974. Clinical Chemistry: Principle and Technique (2nd ed). Harper and Row Inc., Vergenia, USA.

Jintanawit, W., Kanto, U., Juttupornpong, S. and Harinasut, P. 2004. Study on chemical and bromelain activity analysis of pineapple juice and utilization for protein digestion in soybean meal, pp. 26-32. In Proceedings of 42nd Kasetsart University Annual Conference: Animals, Veterinary Medicine. Bangkok. (in Thai)

Jouany, J.P. 2006. Optimizing rumen functions in the close-up transition period and early lactation to drive dry matter intake and energy balance in cows. Animal Reproduction Science 96: 250-264.

Kanebo, J.J. 1980. Appendexes. pp. 785-797. In Kanebo, J.J. Clinical Biochemistry of Domestic Animals. Academic Press, New York.

Namanee, P., Kuprasert, S. and Ngampongsai, W. 2011. Utilization of pineapple waste as roughage source in goats. Khon kaen Agricultural Journal 39: 399-412. (in Thai)

Poathong, S., Sangsai, A., Chobtang, J. and Kaskomalas, S. 2010. Research Report on Feed intake and Nutrient Digestibility of Total Mixed Ration Silage Comprised Different Levels of Pineapple Peels in Goat. Department of livestock development, Ministry of agriculture and cooperatives, Bangkok.

Preston, T.R. and Leng, R.A. 1987. Matching Ruminant Production Systems with Available Resources in the Tropics and Sub-tropics. Penambull Book Publisher, Australia.

Schneider, B.H. and Flatt, W.P. 1975. The Evaluation of Feeding Through Digestibility Experiments. University of Georgia Press, Athens.

Sirijariyawat, A. and Nontaloon, K. 2020. Proteolytic activity of crude extract from different parts of pineapple and papaya. Khon kaen Agricultural Journal 48(Suppl.1): 1087-1092. (in Thai)

Somsuk, C. 1987. Increasing the enzyme activity of bromelain powder from pineapple stems. Master Thesis of Science, Kasetsart University. (in Thai)

Srisaikham, S. 2009. Effects of neem (Azadirachta indica a. juss. var. siamensis valeton) foliage utilization in meat goat diets on rumen fermentation and productive performances. Master Thesis of Science in Animal Production Technology, Suranaree University of Technology. (in Thai)

Tiffany, T.O., Jansen, J.M., Burtis, C.A., Overton, J.B. and Scott, C.D. 1972. Enzymatic kinetic rate and end point analyses of substrate by the use of a GeMSAEC fast analyser. Clinical Chemistry 18: 829-840.

Timklip, C. 2017. Effect of fermented total mixed ration (FTMR) from pineapple wastes on rumen ecology, nutrients digestibility and growth performance in crossbred goat. Master Thesis of Science (Animal Science), Silpakorn University. (in Thai)

Van Soest, P.J., Robertson, J.B., and Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal production. Journal of Dairy Science 74: 3583-3597.

Wanapat, M. 1990. Ruminant nutrition. Department of Animal Science, Faculty of Agriculture, Khon Kaen University Press, Khon Kaen. (in Thai)