Effects of Using Spent Coffee Grounds as a Substrate Supplement for Cultivation of Abalone Mushroom (Pleurotus cystidiosus)

Authors

  • Waraporn Kaewkhon Biology Program, Faculty of Education, Chiang Rai Rajabhat University, Mueang, Chiang Rai 57100, Thailand.
  • Nikhom Naksupan Division of Microbiology and Parasitology, School of Medical Science, University of Phayao, Mueang, Phayao 56000, Thailand.

Keywords:

Mushroom cultivation, Spent coffee grounds, Abalone mushroom, Mycelial growth

Abstract

In Chiang Rai province, the number of coffee shops is increasing, resulting in a greater amount of spent coffee grounds (SCGs) each year. SCGs are a by-product generated by the coffee shop, and their waste has caused increasing environmental problems. Nowadays, there is a growing trend toward reusing SCGs. This study investigates the influence of SCGs as a supplementary substrate for abalone mushroom (Pleurotus cystidiosus) cultivation. The experiment was divided into (1) a control group of 100% sawdust and (2) treatment group with sawdust supplemented with SCGs in different ratios: 0.25% v/v, 0.50% v/v, 1.0% v/v and 2.5% v/v. The results showed that adding SCGs at ratios of 0.25 to 2.5% v/v increased mycelia growth. The highest growth rate was observed with 0.25% v/v and 0.5% v/v SCGs supplementation, compared to the control group (p<0.05). Furthermore, sawdust substrate supplemented with 0.25 to 2.5% v/v SCGs produced a higher yield of abalone mushrooms compared to the control group (p<0.05). These findings indicate that using 0.25% and 0.5% v/v as a supplementary substrate can effectively promote mycelial growth, fruiting body size and yield of abalone mushrooms.

References

Alsanad, M.A., Sassine, Y.N., Sebaaly, Z.E. and Fayssal, S.A. 2020. Spent coffee grounds influence on Pleurotus ostreatus production, composition, fatty acid profile, and lignocellulose biodegradation capacity. CyTA-Journal of Food 19(1): 11-20.

Anantasaran, J., Sae-Liw, N. and Thongkum. T. 2023. Cultivation of abalone mushroom with leftover earleaf acacia sawdust from zebra dove cage making industry. Journal of Science and Technology, Ubon Ratchathani University 25(3): 65-74. (in Thai)

Bunyovimonnat, N., Burakron, J., Jindavutikul, S. and Somrith A. 2019. Increasing nutrition of oyster mushrooms using waste coffee grounds. Bulletin of Applied Sciences 8(8): 96-105. (in Thai)

Campos-Vega, R., Loarca-Piña, G., Vergara-Castañeda, H.A. and Oomah, B.D. 2015. Spent coffee grounds: A review on current research and future prospects. Trends in Food Science & Technology 45(1): 24-36.

Chaichan, W., Chaichan, W. and Sawain, A. 2018. Research report on plant pots from co-production of spent coffee grounds, shell lime and rubber wood sawdust. Rajamangala University of Technology Srivijaya, Trang. (in Thai)

Chulaka, P., Kaewsorn, P. and Jeenprosom, P. 2014. Effect of growing media containing coffee ground on germination and growth of tomato seedling (Lycopersicon esculentum Mill.). Agricultural Science Journal 45(2): 349-352. (in Thai)

Deepalakshmi, K. and Mirunalini, S. 2014. Pleurotus ostreatus: an oyster mushroom with nutritional and medicinal properties. Journal of Biochemical Technology 5(2): 718-726.

Febrian, P.A. and Ismail, Y. 2021. The study of composting from spent coffee grounds in making process liquid fertilizer. Journal of Environmental Engineering and Waste Management 6(2): 111-128.

Kaewkhon, W., Naksupan, N., Seethi, I., Taomun, P., Phetklanphai, S. and Lagsamongkol, K. 2020. Development of abalone mushroom cultivation using dried-corn cobs and floating mass as a supplement for mushroom substrate. In Chandeying, V. ed. Symposium on The 9th National Science Research Conference. University of Phayao, Phayao.

Kaewkhon, W., Manopattarakul, S., Thmmawong, T., Chaiyong, T., Kwirachai, T. and Naksupan, N. 2022. Effect of spent coffee grounds with rubber wood sawdust substrates on the mycelial growth and primordial formation of Agrocybe cylindracea Maire, pp. 598-606. In Mongkolaussavarat, T. ed. Symposium on The 14th National Science Research Conference. University of Phayao, Phayao.

Kamsan, T., Kiddee, P. and Wonglom, P. 2021. Use of coffee ground waste with rubber wood sawdust for golden oyster mushroom (Pleurotus citrinopileatus Singer) cultivation material. PSRU Journal of Science and Technology 6(2): 86-97. (in Thai)

Khan, M.A., Amin, S.M.R., Uddin, M.N., Tania, M. and Alam, N. 2008. Comparative study of the nutritional composition of oyster mushrooms cultivated in Bangladesh. Bangladesh Journal of Mushroom 2: 9-14.

Raman, J., Jang, K.Y., Oh, Y.L., Oh, M., Im, J.H., Lakshmanan, H. and Sabaratnam, V. 2021. Cultivation and nutritional value of prominent Pleurotus spp.: an overview. Mycobiology 49(1): 1-14.

Rodrigues, R., Oliveira, M.B.P.P. and Alves, R.C. 2023. Chlorogenic acids and caffeine from coffee by-products: a review on skincare applications. Cosmetics 10(12): 1-15.

Srijumpa, N., Khayankarn, S. and Chaichuchote, S. 2018. Research Report on Using Coffee Pulps as Substrates for Cultivation of Pleurotus spp., Volvariella volvacea and Coprinus fimetarrius. Chiangrai Horticulture Research Center, Chiangrai.

Tuntiwiwattanapun, N., Usapein, P. and Tongcumpou, C. 2017. The energy usage and environmental impact assessment of spent coffee grounds biodiesel production by an in-situ transesterification process. Energy for Sustainable Development 40: 50-58.

Abalone mushrooms

Published

2025-08-28

How to Cite

Kaewkhon, W., & Naksupan, N. (2025). Effects of Using Spent Coffee Grounds as a Substrate Supplement for Cultivation of Abalone Mushroom (Pleurotus cystidiosus). Recent Science and Technology, 17(3), 262649. retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/262649