การศึกษาวิธีฟอกฆ่าเชื้อผิวของกุหลาบแคระญี่ปุ่นโฮโฮเอมิ (Rosa ‘Hohoemi Rouge’)

Thiwthong  Thongpraphai; Rodjanacorn  Chuengpanya; Apiradee  Siangsuepchart

Authors

Thiwthong Thongpraphai Department of Agroforestry, Maejo University Phrae Campus, Rong Kwang, Phrae 54140, Thailand.
Rodjanacorn Chuengpanya Independent academician in plant tissue culture, 103/1 moo 3, Sara, Kho Kha, Lampang, 52130, Thailand. https://orcid.org/0000-0002-2894-632X
Apiradee Siangsuepchart Department of Agro-Industrial Biotechnology, Maejo University Phrae Campu

Keywords:

Japanese Mini Rose Hohoemi, economic plant, surface sterilization

Abstract

Japanese Mini Rose Hohoemi or Hohoemi rose (Rosa ‘Hohoemi Rouge’) is a rose cultivar that produces blooms throughout the year. This plant is widely used ornamentally because it produces a large number of flowers per branch and its exceptional resistance to plant diseases. It is essential to adjust the number of plants in order to consistently satisfy the ornamental plant market’s requirements. For this reason, tissue culture can help achieve this objective. However, no tissue culture report on Hohoemi rose has been found. Therefore, investigating a suitable surface sterilization method for achieving large numbers of in vitro axenic plantlets is crucial. In this current study, young branches of Hohoemi rose were subjected to four distinct procedures for surface sterilization using Hiter^® or Osil^® solutions. After that, nodes were subsequently excised and cultured onto Murashige and Skoog (1962) medium. The contamination-free rate of the explants and the survival rate of the sterilized explants were recorded after 4 and 8 weeks of culture, respectively. The results revealed that surface sterilization with 10% (v/v) of Osil^® for 10 min of exposure time was the appropriate disinfection method. High rates of contamination-free (90%) and survival (100%) were achieved with this treatment. The results of this study will be helpful in producing large numbers of axenic cultures in vitro following the surface sterilization process, which will facilitate future research and the development of a successful tissue culture technique for Hohoemi rose.

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