Evaluation of a Commercial β-Mannanase for Konjac Glucomannan Hydrolysis: Activity and Molecular Weight Analysis
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Abstract
β-Mannanase (endo-1,4-β-D-mannanase) is a glycoside hydrolase with potential for producing mannooligosaccharides (MOS), which are recognized for their prebiotic properties. However, the applicability of commercial enzymes to specific substrates such as konjac glucomannan (KGM) remains insufficiently evaluated. This study investigated the hydrolytic activity and estimated the molecular weight of a commercial β-mannanase (Mannanase BGM “AMANO” 10) toward KGM. The enzyme exhibited measurable activity under the tested conditions, with a specific activity of approximately 100,574 U g-1 protein and a corresponding reducing sugar yield. SDS-PAGE analysis indicated an apparent molecular weight in the range of 50–75 kDa. These findings provide preliminary insights into the performance of the commercial enzyme on KGM and establish baseline information for its potential application in MOS production.
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