Screening of Exopolysaccharide-Producing Drought Tolerant Rhizobacteria from Orchard Soils in Chanthaburi Province
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Abstract
Exopolysaccharide production of rhizobacteria strongly affects plant growth and drought tolerance. This work aims at evaluating the ability of bacteria previously isolated from the dry region of orchard soils in Chanthaburi province to produce catalase, oxidase and screening exopolysaccharide producing drought tolerant rhizobacteria. The findings revealed all 19 isolates were capable of producing catalase, while 8 isolates showed ability to generate oxidase. Furthermore, all 19 isolates exhibited exopolysaccharide production when cultured in a mineral salt medium (MS) supplemented with 15% w/v PEG 6000 to simulate drought stress. The results were confirmed using the modified alcian blue dye test, with percentages ranging from 6.76 to 45.02. Isolate MuD01-2, a gram-positive bacillus with a mucoid colony, was selected due to the highest EPS production (45.02 ± 1.15%). Additionally, isolate MuD01-2 presented high tolerance to water stress with a cell number of 9.47 ± 0.30 log CFU/ml after 6 days culturing in Tryptic Soy Broth (TSB) supplemented with 15% (w/v) PEG 6000. The results of water stress tests were not significantly different from those of the control without PEG 6000 (9.23 ± 0.15 log CFU/ml, p < 0.05). MUD01-2 showed high potential to be developed as a drought bioinoculant for application in orchard soils.
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