Effects of Culture Media on the Developments of Lateral Buds of Vanilla (Vanilla planifolia Andr.)

Abstract

The lateral buds of vanilla (Vanilla planifolia Andr.) were cultured to induce the maximum number of multiple shoot buds at the laboratory of Biotechnology Research and Development Office during 1999-2001. The experiment was designed as CRD with 6 treatments and 10 replications. The lateral buds grown on Murashige and Skoog (MS) media supplementrd with 0,1,2,3,4 and 5 mg/l of BA+0.6 mg/l of NAA were assessed to determine the media induce multiple shoot buds, the maximum number of shoot buds initiation and development. The highest number of shoot buds produce 14 shoots per lateral bud, the mean of shoot length and leaf number per shoot were 2.41 cm and 3.2 leaves, and the greenish of vigorous shoots from MS+BA 3 mg/l + NAA 0.6 mg/l medium after 10 mounts in culture. Multiple shoot buds were sub-cultured of the microcutting on MS+BA 3 mg/l + NAA 0.6 mg/l + activated charcoal 2 gm/l medium to induce rooting and vigorous plantlets for transplanting to the greenhouse. In vitro culture techniques can be propagated for the production of a large number of plantlets from 1 bud at a short time.