Improvement of New Hybrid Pleurotus sp. Bhutan Strains Using Di-mon Mating Technique

Authors

  • Ratchadaporn Thonghem Biotechnology Research and Development Office, Department of Agriculture
  • Suvalux Chaichuchote Biotechnology Research and Development Office, Department of Agriculture
  • Tharinee sukomol Biotechnology Research and Development Office, Department of Agriculture
  • Upathum Oonjai Biotechnology Research and Development Office, Department of Agriculture
  • Thanchanok Natai Pluak Daeng Rayong Province Development Service Center Under His Majesty’s Initiatives District
  • Srirat Bupato Kung Krabaen Bay Royal Development Study Centre ,Khlong Khut, Tha Mai District, Chanthaburi
  • Phattaya Thepdet Royal-initiated Phu Sing Agricultural Development Centre District, Sri Sa Ket

DOI:

https://doi.org/10.14456/thaidoa-agres.2017.7

Keywords:

Di-mon mating, monokaryotic mycelium, dikaryotic mycelium

Abstract

Improvement of Pleurotus sp. mushroom (Bhutan strain) was done by Di-mon mating technique using dikaryotic mycelium from strain No. 3 (P3) and monokaryotic mycelium from 8 isolates (A15 - A22) Eighteen hybrid strains were obtained. After cultivating in 800 g of sawdust plastic bags at mushroom house (Bangkok) during  November 2014 - February 2015, all hybrids produced the fruiting bodies. Whereas,
cultivation between March and June 2015, 8 hybrid strains formed the fruiting bodies and only 15 hybrid strains produced the fruiting bodies when cultivated during July – October 2015. Three hybrids, P3xSE5, P3xSA6 and P3xSB24 gave higher yield than others and were selected for further studies. The mentioned three hybrids were cultivated in the 3 areas of Royal Project. Yield of those hybrids were compared to yield of P3 strain. Results revealed that P3xSB24 was suitable for cultivation in the Royal-initiated Phu Sing Agricultural Development Centre, Srisaket Province, with yield 216.82 g/bag. The 3 hybrids strains were suitable for cultivation in Pluak Daeng, Rayong Province Development Service Center Under His Majesty’s Initiatives. Yields of the 3 hybrid strains were significantly higher than P3 in two cultivation periods. Cultivation in Kung Krabaen Bay Royal Development Study Center, Chanthaburi Province, P3xSE5 produced the highest yield during December 2015 – March 2016, which was 171.43 g/bag.

Published

2017-12-31

How to Cite

Thonghem, R. ., Chaichuchote, S. ., sukomol, T. ., Oonjai, U. ., Natai, T. ., Bupato, S. ., & Thepdet, P. . (2017). Improvement of New Hybrid Pleurotus sp. Bhutan Strains Using Di-mon Mating Technique. Thai Agricultural Research Journal, 35(3), 257–269. https://doi.org/10.14456/thaidoa-agres.2017.7

Issue

Section

Technical or research paper