Cryopreservation Technique of Taro (Colocacia esculenta) Germplasm Using Vitrification Method

Authors

  • Padnaree Rukkid Biotechnology Research and Development Office, 85 M.1, Rangsit-Nakhonnayok Rd., Thanyaburi, Pathum Thani, 12110 Thailand Tel. +662 904 6885-95 Mobile +668 119 69745
  • Phatchara Piriyavinit
  • Kunyaporn Pipithsangchan
  • Sunisa Khatpaeng

DOI:

https://doi.org/10.14456/thaidoa-agres.2021.15

Keywords:

In vitro conservation, micropropagation

Abstract

Taro (Colocacia esculenta (L.) Schott) is a tropical root crop that can be cultivated in all regions of Thailand. Currently the taro’s genetic resources are being conserved in the field collection at Phichit Agricultural Research and Development Center, Department of Agriculture. Cryopreservation is considered to be a more cost saving option for long term conservation of vegetatively propagated crops and can also reduce the cumbersome maintainance of large amount of taro from season to season. This research aimed to conserve two non-aromatic (Phueak Khai (P1) and Phueak Aoi (P2)) and two aromatic (Phueak Hom THA-104 (P3) and Phueak Hom Phayao (P4)) taro germplasms using
cryopreservation approach. The apical meristems of the 4 taros were cultured on 12 Murashige and Skoog (MS) based medium supplemented with the combinations of different concentrations of benzyladenine acid (BA) (0, 3, 5 and 7 mg/l) and naphthalene acetic acid (NAA) (0, 1 and 2 mg/l) for 16 weeks. Results showed that MS medium supplemented with 5 mg/l BA and 2 mg/l NAA was suitable for the two non-aromatic taro cultures which provided the highest shoot number averages at 10.25 and 9.19 shoots per explant for P1 and P2, respectively. For aromatic taro cultures, P3 and P4 showed the highest shoot number averages on MS medium supplemented with 5 mg/l BA and 1 mg/l NAA at 10.04
and 9.57 shoots per explant, respectively. The taro shoots obtained from the 4 cultivars were further investigated for possible conservation under the cryopreservation condition using vitrification method. Results revealed that the optimum condition for taro cryopreservation was by dehydrating the taro shoots in plant vitrification solution 3 (PVS3) at 25° ํC for 30 minutes which showed the average recovery rates of 49.5, 64.1, 52.7 and 59.5 % of P1-P4, respectively after 4-weeks cultured.

Downloads

Download data is not yet available.

References

กรมส่งเสริมการเกษตร. 2564. ระบบให้บริการข้อมูลสารสนเทศการผลิตทางด้านการเกษตร กรมส่งเสริมการเกษตร กระทรวงเกษตรและสหกรณ์. แหล่งข้อมูล: http://production.doae.go.th/service/login.สืบค้น: 20 เมษายน 2564

กรมศุลกากร. 2564. รายงานสถิติ กรมศุลกากร. แหล่งข้อมูล: http://www.customs.go.th/statistic_report.php.

สืบค้น: 20 เมษายน 2564

นิรนาม. 2557. เผือก. ฐานความรู้ด้านพืช กรมวิชาการเกษตร. แหล่งข้อมูล: http://www.doa.go.th/

pl_data/TARO/STAT/st1.html. สืบค้น: 25 เมษายน 2560

พีรเดช ทองอำไพ. 2537. ฮอร์โมนพืชและสารสังเคราะห์ แนวทางการใช้ประโยชน์ในประเทศไทย, พิมพ์ครั้งที่ 4, วี.บี. บุ๊คเซนเตอร์, ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์: กรุงเทพฯ. 196 หน้า.

Acedo, V.Z., O.P. Damasco, A.C. Laurena, P.C. Sta Cruz, L.O. Namuco and A.G. Lalusin. 2018. Shoot tip splitting for rapid micropropagation of Philippine taro (Colocasia esculenta (L.) Schott). Int. J. Adv. Agri. Res. 6: 38-46.

Best, B.P. 2015. Cryoprotectant Toxicity: Facts, Tssues and Questions. Rejuvenation Res. 18(5): 422-436.

Buitink, J. and O. Leprince. 2004. Glass formation in plant anhydrobiotes: survival in the dry state. Cryobiology 48(3): 215-228.

Chung, R.C. and C.J. Goh. 1994. High frequency direct shoot regeneration from corm axillary buds and rapid clonal propagation of taro (Colocasia esculenta var. esculenta (L.) Schott (Araceae)). Plant Sci. 104: 93-100.

Cordova, L.B. and K. Thammasiri. 2016. Cryopreservation on a cryo-plate of Arundina graminifolia protocorms, dehydrated with silica gel and drying beads. Cryo Lett. 37(2): 68-76.

Engelmann, F. 2000. Development of cryopreservation techniques. pp. 8-20. In Englemann, F. and Takagi, H. (eds.) Cryopreservation of Tropical Plant Germplasm Current Research Progress and Application. IPGRI, Rome.

Hong, S., M. Yin; X. Shao, A. Wang and W. Xu. 2009. Cryopreservation of embryogenic callus of Dioscorea bulbifera by vitrification, Cryo Lett. 30(1): 64-75.

Hutami, S. and R. Purnamaningsih. 2013. Shoot multiplication of taro (Colocasia esculenta var. Antiquorum) through in vitro culture. pp. 35-40. In: Proceeding International Conference 2013 The 4th Green Technology Facculty of Science and Technology Islamic of University State Maulana Malik Ibrahim Malang. Indonesia.

Kartha, K.K., N.L. Leung and K. Pahl. 1980. Cryopreservation of plantlets. J. Am. Soc. Hortic. Sci. 105: 481-484.

Kim, H.H., J.W. Yoon, Y.E. Park, E.G. Cho, J.K. Sohn, T.S. Kim and F. Engelmann. 2006. Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification. Cryo Lett. 27: 223-234.

Ko, C.Y., J.P. Kung and R. Mc Donald. 2008. In vitro micropropagation of white dasheen (Colocasia esculenta). Afr. J. Biotechnol. 7(1): 41-43.

Matsumoto, T., A. Sakai, C. Takahashi and K. Yamada. 1995. Cryopreservation of in vitro grown apical meristems of wasabi (Wasadia japonica) by encapsulation-vitrification method. Cryo Lett. 16: 189-196.

Mohanty, P., M.C. Das, S. Kumaria and P. Tandon. 2012. High-efficiency cryopreservation of the medicinal orchid Dendrobium nobile Lindl. Plant Cell Tissue Organ Cult. 109(2): 297-305.

Nath, V.S., M.S. alias Sankar, V.M. Hegde, M.L. Jeeva, R.S. Misra and S.S. Veena. 2012. A simple and efficient protocol for rapid regeneration and propagation of taro (Colocasia esculenta (L.) Schott.) in vitro from apical meristems. Int. J. Plant Dev. Biol. 6(1): 64-66.

Preetha, T.S., A.S. Hemanthakumar and P.N. Krishnan. 2013. Shoot tip Cryopreservation by Vitrification in Kaempferia galanga L. An endangered, over exploited medicinal plant in Tropical Asia. J. Pharm. Biol. Sci. 8(3): 19-23.

Reed, B.M. 2008. Plant Cryopreservation: A Practical Guide, Springer, New York. 542 p.

Sakai, A., S. Kobayashi and Oiyama, I. 1990. Cryopreservation of nucellar cells of navel organe (Citrus sinensis Osb. Var. brasiliensis Tanaka) by vitrification. Plant Cell Rep. 9(1): 30-33.

Sant, R., B. Panis, M. Taylor and A. Tyagi. 2008. Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculenta var. esculenta) accessions. Plant Cell Tiss Organ Cult. 92: 107-111.

Taiz, L. and E. Zeiger. 1998. Plant Physiology, in Chapter 21, 2nd Ed, Sinauer Associates, U.S.A. 792 p.

Takagi, H., N.T. Thinh, O.M. Islam and T. Senboku. 1997. Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure. Plant Cell Reports. 16: 594-599.

Underwood, L., J. Solocinski, E. Rosiek, Q. Osgood and N. Chakraborty. 2019. Active modulation of Hydrogen bonding by sericin enhances cryopreservation outcomes. brioRxiv. Available at: https://doi.org/10.1101/773721. Accessed: 29 August, 2019

Vaurasi, V. and R. Kant. 2016. Effects of salinity and plant growth media on in vitro growth and development of taro (Colocasia esculenta L.) varieties. Acta Horticulturae et Regiotecturae. 1: 17-20.

Wang, J.K. 1983. Taro, a review of Colocacia esculenta and its potentials. University of Hawaii Press, Hawaii. 400 pp.

Wang, Q., O. Batuman; P. Li, M.B. Joseph and R. Gafny. 2002. A simple and efficient cryopreservation of in vitro-grown shoot tips of ‘Troyer’ citrange [Poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-vitrification. Euphytica 128: 135-142.

Yang, J., N. Cai, H. Zhai, J. Zhang, Y. Zhu and L. Zhang. 2016. Natural zwitterionic betaine enables cells to survive ultrarapia cryopreservation. Available at: www.nature.com/scientificreports. Accessed: 2 March, 2020

Published

2021-05-05

How to Cite

Rukkid พ., Piriyavinit พ. ., Pipithsangchan ก. ., & Khatpaeng ส. . (2021). Cryopreservation Technique of Taro (Colocacia esculenta) Germplasm Using Vitrification Method. Thai Agricultural Research Journal, 39(2). https://doi.org/10.14456/thaidoa-agres.2021.15

Issue

Section

Technical or research paper