การระบุชนิดของเห็ดยานางิ (Cyclocybe spp.) ด้วยลักษณะทางสัณฐานวิทยา และข้อมูลลำดับนิวคลีโอไทด์บริเวณ ITS

Jittra Kittimorakul; Vipavee Chanroj; Ratchadaporn Thonghem; Paranee Sawangsri

Authors

Jittra Kittimorakul Biotechnology Research and Development Office, Rangsit-Nakhon Nayok Rd., Thanyaburi, Pathum Thani 12110, Thailand
Vipavee Chanroj Biotechnology Research and Development Office, Rangsit-Nakhon Nayok Rd., Thanyaburi, Pathum Thani 12110, Thailand
Ratchadaporn Thonghem Biotechnology Research and Development Office, Rangsit-Nakhon Nayok Rd., Thanyaburi, Pathum Thani 12110, Thailand
Paranee Sawangsri Biotechnology Research and Development Office, Rangsit-Nakhon Nayok Rd., Thanyaburi, Pathum Thani 12110, Thailand

Keywords:

identification, Yanagi mushroom, morphology, ITS region sequences

Abstract

The Yanagi mushroom (Cyclocybe spp.) is one of the important mushrooms widely cultivated. Species identification of this mushroom in Thailand was confused. The objective of this research was to investigate morphological characteristics and identify ITS region sequences of Yanagi mushroom strains for correct identification. Nineteen strains of Yanagi mushrooms were collected from 10 different locations for the study. The results revealed that 18 strains showed brown or light brown to cream on the cap, cylindrical stalk, and off-white surface. After the cap was open, the partial veil was quite short seeming like C. cylindracea. While the Yanagi mushroom strain Ya11 was different, the cap color was brown to dark brown with tiny white scales, the stalk was cylindrical, the surface was light brown, and the partial veil was thicker and larger, seeming like
C. chaxingu. Furthermore, the nucleotide sequence analysis of the ITS region and phylogenetic analysis separated 19 Yanagi mushroom strains into two groups according to the morphological study. Thus, the identification of the Yanagi mushroom using both morphological characteristics and ITS region sequences was an accurate and efficacious method for use in the species identification of this mushroom in Thailand.

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