Induction of Embryogenic Callus in Durian via Tissue Culture

Abstract

Embryogenic callus can be induced from ovule seeds of Durio zibethinus Murr., variety Montong. The appropriate fruit ages wee 8-20 days or 1-3 weeks after anthesis. The older and the younger ones were not induced embryogenic callus. Callus can be obtained from 2 different kinds of induction media, one has been Murashige & Trucker basal medium with 500 mg/.l malt extract, 0.1 mg/I IAA, 1 mg/I IAA, 1 mg./kinetin (Nu 5) and another one has been Murashige & Skoog basal medium with 1 mg./I NAA(MSN). Subculture every 3 weeks can be reduced the phenolic compound productions and obtained to maximum of embryogenic callus proliferation. This technique was also suitable for embryogenic callus in Durio giffithii (Mast.) Bakh., the wild type of durian in Thailand.