Stability of Bioactive Compounds and Wound Healing Activities of Porcine Placental Extract

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Published: Oct 14, 2025
Keywords:
Porcine Placental Extract Growth Factors VEGF-A Wound Healing Endothelial Cells

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Nutthamon Kijchalao
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
Itsariya Chuaichunu
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
Arunya Jiraviriyakul
Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand
Panthip Rattanasinganchan
Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand
Penpak Moolthiya
Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand
Kittipat Sopitthummakkhun
Faculty of Science and Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand
Pornphimon Metheenukul
Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, 10900, Thailand
Tuangporn Suthiphongchai
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Rutaiwan Tohtong
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Kyaw Zwar Myint
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Suchada Phimsen
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand

Abstract

Porcine placental extract (PPE) is a biologically active substance enriched with proteins and regenerative mediators, offering therapeutic potential in wound healing applications. However, the long-term stability of PPE under various storage conditions remains unclear. This study investigated the biochemical stability and functional bioactivity of PPE stored at 4 °C and −20 °C for 1, 3, 6, and 12 months. Total protein content was quantified using the Bradford assay, while levels of vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor-BB (PDGF-BB) were measured using enzyme-linked immunosorbent assay (ELISA). Functional assays included endothelial cell proliferation using the MTT assay and migration assessed by scratch wound healing assay on EA.hy926 cells. Results showed that total protein content declined significantly after as early as one month, with more than 50% reduction by 12 months at both temperatures. VEGF-A levels remained stable for one month before decreasing, whereas PDGF-BB levels dropped significantly from the first month. Despite these biochemical losses, PPE maintained stable bioactivity in promoting endothelial cell proliferation and migration throughout the 12-month period, regardless of storage temperature. These findings demonstrate that PPE retains functional efficacy for at least 12 months under refrigerated or frozen storage and support its potential development as a stable, bioactive wound-healing agent. Improved preservation techniques may further enhance its long-term biochemical integrity.

Article Details

Issue
Vol. 5 No. 2 (2025): July-December
Section
Research Articles
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