Effects of elicitors on adenosine and cordycepin production of Cordyceps militaris
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Abstract
The purpose of this research was to observe the effects of different concentrations of elicitors on mycelial growth, fruiting body formation, physical feature and production of adenosine and cordycepin of Cordyceps militaris. The experimental design was Completely Randomized Design (CRD). There were 9 treatments with 3 replications including yeast extract at concentrations of 5 10 15 and 20 g/l and methyl jasmonate solution at concentrations of 50 100 150 and 200 µM and control. Results showed that the mycelial fresh weight and the percentage of dry weight were not significantly different (p>0.05). Whereas, the mycelium which was cultured on the Modified of Potato Dextrose Broth (MPDB) MPDB 3 medium supplemented with 10 g/l yeast extract gave significant higher yield than other treatments in dry weight (p<0.05). The fruiting body numbers, fresh weight and dry weight of the fungi grown on the Artificial Modified of Potato Dextrose Broth (A-MPDB) A-MPDB 6 medium supplemented with 50 µM methyl jasmonate solution gave the highest value (p<0.01). The mean values of brightness (L*), red (a*), yellow (b*) and Hue angle of fruiting body cultured on the A-MPDB 6 medium gave 70.46±2.00, 18.29±0.52, 49.59±1.56 and 69.75±0.41, respectively. The bioactive analysis revealed that the A-MPDB 6 medium gave the highest adenosine content of 47.73±6.78 mg/100 g and that of A-MPDB 7 medium supplemented with 100 µM methyl jasmonate solution gave the highest cordycepin content of 326.45±34.28 mg/100 g.
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