Effects of Supplementation of Melaleuca cajuputi Extract to Control Vibrio Pathogen in Litopenaeus vannamei Aquaculture
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Abstract
The objective of this research was to investigate the effect of supplementation of Melaleuca cajuputi extractin ready-made feed to control vibrio spp. pathogen in Litopeneaus vannamei aquaculture. M. cajuputi leaves was extract with ethanol, then the solvent was removed by the rotary under vacuum evaporated and dried by freeze dry technique. The extract powder was prepared as a dietary supplement at a concentration of 300 ppm. Ethanol and water in a ratio of 8:2 was dissolved the extract well and significantly contain the highest amout of total phenolic content 11.48±0.00 mg GAE/mg extract, total flavonoid content 153.27±0.20 mg RE/mg extract and DPPH and ABTS free radicals inhibition with 90.21±0.35% และ 99.52±0.08%, respectively. The extract was sprayed on ready-made feed in a ratio of 50 milliliters to 1 kilogram of feed. The results of water quality monitoring and vibrio in white shrimp showed that the water in the experimental pond showed higher number of green and yellow bacterial colonies than that of control unit, but the amount of green and yellow bacterial colonies in the experimental shrimp was less than that of the control unit. It has been shown that M. cajuputi extract affects the response to resistance to Vibrio pathogens and can be developed as a dietary supplement to enhance health in L. Vannamei and reduce infection with Vibrio spp. pathogens.
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