INVESTIGATION OF TREMATODE METACERCARIAE IN FERMENTED FISH FROM UDON THANI AND KALASIN PROVINCES, THAILAND
Food-borne disease is an important public health problem in South East Asia including Opisthorchaiasis which endemic in Thailand, Laos PDR and Cambodia. Infective stage of Trematode is the metacercariae which encysted in the muscles of Cyprinoid fish. The parasite transmission is caused by consuming of uncooked
or raw infected fish. In this study, two products of fermented fish were collected from five districts of Udon Thani province and seven districts of Kalasin province. All samples were digested with 0.25% pepsin solution with 37°C for 1 hour, after that filtered through the various sizes of sieves. The trematode metacercariae were identified under a stereo microscope and a compound microscope. A total of 31 metacercariae (Mc) were found in Pla-ra (9 Mc) and Pla-som (22 Mc) with shorter fermentation period which collected from Kumphawapi Kumphawapi, Mueang Udon Thani, and Kut Chap districts. The average numbers of metacercariae were 0.038 Mc/g to 0.006 Mc/g. The percentage of positive sample sites was 9.1 % (1/11) for Pla-ra and 42.9% (3/7) for Pla-som. The infected fish species were Barbodes aurotaeniatus and Barbonymus gonionotus. This observation is useful for the public health organization in terms of parasitic disease
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