• Suphod Tabtimyai
  • Kongsak Boonyapranai
  • Laddawan Phaworn Nakhonratchasima College
  • Onuma Chansatein


Marigold Flowers, Phytochemical, Antioxidant


This research aimed to study was compared to other processes including aqueous decoction,
maceration and Soxhlet extraction on the photochemical contents, antioxidant activities and Antidenaturation activity of marigold flowers (Rocco Deep Gold). The results showed that 95% ethanol
Soxhlet extraction of flowers contained higher content of phenolics and carotenoids than the other
extracts (63.9±0.01 mg GAE/g extract and 103.95 mg/g extract, respectively). Fifty percentage ethanol
maceration of flowers also contained high levels of flavonoids (174.30±0.07 mg/g extract). In regard
to the antioxidant of DPPH radical scavenging. Ninety-five percentage ethanol Soxhlet extraction
extract of flowers showed stronger inhibition effect than other extracts. (IC50=0.45±0.00 mg/ml). The
in vitro anti-Denaturation activity that aqueous extracted has the highest % inhibition, followed by
95% ethanol Soxhlet extraction and 50% ethanol maceration. the inhibitory activity was 74.44%,
54.30% and 38.67%, respectively.


Download data is not yet available.


Aisantia. O., & Wongkrachang, K. (2014). The Investigation of the Extraction Solvent System of Total Phenolics and Flavonoids–Rich Extracts and Antioxidant Activity from Tagetes erecta Flower. Science and Technology Nakhon Sawan Rajabhat University Journal, 7(7), 29-40. (In Thai)

Buachoon, N., & Manjit, W. (2018). Quantitation of Total Phenolics Antioxidant Activity and Development of skin care lotions. VRU Research and Development Journal Science and Technology, 13(2), 86-97. (In Thai)

Burlec, A.F., Cioanca, O., Mircea, C., Arsene, C., Tuchiluş, C., Corciovă, A., & Hăncianu, M. (2019). Antioxidant and antimicrobial properties of Chrysanthemum and Tagetes selective extracts. Farmacia, 67, 405–410.

Chompoo, J., U-yatung, S., Kaiput, T., & Boonruangrod, R. (2021). Phytochemical Contents and Antioxidants Activity of Extracts from French Marigold Flowers (Tagetes patula L.). King Mongkut’s Agricultural Journal, 39(4), 264-273.

Folin, O., & Ciocalteu, V. (1927). On tyrosine and tryptophane determinations in proteins. Journal of

Biological Chemistry, 73, 627-650.

Ingkasupart, P., Manochai, B., Song, W.T., & Hong, J.H. (2015). Antioxidant activities and lutein content of 11 marigold cultivars (Tagetes spp.) grown in Thailand. Food Science and Technology (Campinas), 35(2), 380-385. (In Thai)

Isantea, O., & Wongkrajang, K. 2015. The Investigation of the Extraction Solvent System of Total Phenolics and Flavonoids–Rich Extracts and Antioxidant Activity from Tagetes erecta Flower. Science and Technology Nakhon Sawan Rajabhat University Journal, 7(7), 29-41. (In Thai)

Kaisoon, O., Konczak, I., & Siriamornpun, S. (2012). Potential health enhancing properties of edible flowers from Thailand. Food Research International, 46 563–571.

Karwani, G., & Sisodia, S.S. (2015). Tagetes erecta plant: Review with significant pharmacological activities. World Journal of Pharmaceutical Sciences, 3(6), 1180-1183.

Niyomdecha. A., & Khongsen. M. (2013). Metabolism and Nutritional Values of Carotenoids on Egg Yolk Color. Princess of Naradhiwas University Journal, 5(4), 112-121. (In Thai)

Pietta, P.G. (2000). Flavonoidsasantioxidants. Journal of Natural Products, 63, 1035-1042.

Pourmorad, F., Hosseinimehr, S.J., & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11), 1142-1145. (In Thai)

Phrompittayarat, W., Putalun, W., Tanaka, H., Jetiyanon, K., Wittaya-areekul, S. & Ingkaninan, K. (2007). Comparison of various extraction methods of Bacupa monnieri. Naresuan University Journal, 15(1), 29-34.

Pérez-Ortega, G., Angeles-López, G.E., Argueta-Villamar, A., & González-Trujano, M.E. (2017). Preclinical evidence of the anxiolytic and sedative-like activities of Tagetes erecta L. reinforces its ethnobotanical approach. Biomedicine and Pharmacotherapy, 93 (Supplement C), 383-390.

Ranganna, S. (1999). Handbook of analysis and quality control for fruit and vegetable products. New Delhi.Tata Mc-Graw Hill publishing company Ltd.

Saani, M., Lawrence, R., & Lawrence, K. (2017). Evaluation of pigments from methanolic extract of Tagetes erecta and Beta vulgaris as antioxidant and antibacterial agent. Natural Product Research, 11, 1-4.

Singsai, K., Sakdavirote, A., Wechpanishkitkul, K., & Moonsamai, A. (2020). The comparison of phenolic compounds, flavonoids and antioxidant activities of the ethanolic extracts of shoots, leaves, fruits and seeds of Leucaena leucocephala. Naresuan Phayao Journal, 13(3), 66-73. (In Thai)

Williams, L.A., O’Connar, A., Latore, L., Dennis, O., Ringer, S., Whittaker, J.A. (2008). The in vitro antidenaturation effects induced by natural products and nonsteroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of antiinflammatory compounds, without the use of animals, in the early stages

of the drug discovery process. West Indian Medical Journal, 57(4), 327-331.

Wang, W., Xu, H., Chen, H., Tai, K., Liu, F., & Gao, Y. (2016). In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetaget in extracted from marigold (Tagetes erecta L.) inflorescence residues. Journal of Food Science and Technology, 53(6), 2614-2624.

Wongkrajang, K., & Jankam, A. 2017. The Effect of Ethanol Extract of Tagetes erecta Flowers on Lettuce Growth. Science and Technology Nakhon Sawan Rajabhat University Journal, 9(9), 97-111. (In Thai)

Xu, L. W., Chen J., Huan, H. Y., & Shi, Y. P. (2012). Phytochemicals and their biological activities of plants in Tagetes. Journal Chinese Herbal Medicines, 4(2), 103-117.

Yen, G.C., & Hsieh, C.L. (1997). Antioxidant effects of dopamine and related compounds. Bioscience, Biotechnology, and Biochemistry, 61(10), 1646–1649.

Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555-559.





บทความวิจัย (Research Article)