Egg Consumption Impacts Serum Proteome Profiles and Metabolic Pathways in Obese Men
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Abstract
There are inconclusive data from epidemiological studies linking egg consumption to an increased prevalence of cardiovascular disease. This is the first study using the proteomic approach to analyze the serum proteome profile and potential metabolic pathways related to difference egg intake, compared between the control with normal body mass index (N=116) and the obese groups (N=217). Clinical assessment, food frequency questionnaire, biochemical analysis were performed and serum proteomic was analyzed by LC/MS-MS. Significant findings from comparison between the controls with low egg intake and the obese with high egg intake indicated relative fold changes of log 2 of upregulated and downregulated proteins, including Tumor necrosis factor receptor superfamily member 18, NF-kappa-B-activating protein, Complement C1s subcomponent, and Collagen alpha-6(VI) chain. Further analysis for protein-protein interaction of unique proteins (N=238) from obese group with high egg intake demonstrated association with mTOR, AMPK, HIF-1, and insulin signaling pathway. In conclusion, changes in clinical and biochemical markers in obesity were linked to potential pathways identified from proteomics and these results provided data for consideration in the development of dietary guidelines for egg consumption among healthy and metabolic risk groups.
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