Production of Fiber Hydrolysate from Bamboo Shoot with Antioxidative Properties by Enzymatic Hydrolysis

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Supatra Karnjanapratum
Pensiri Kaewthong
Sirima Takeungwongtrakul
Thanasak Sae-leaw
Jeong Hwa Hong
Sitthipong Nalinanon*

Abstract

Bamboo shoots possess a rich source of dietary fiber for Asian countries as well as Thailand, which give various beneficial physiological effects for human beings. Dietary fibers in soluble form could provide better texture and would be easier to apply in food products. This study aimed to prepare fiber hydrolysate with high solubility and antioxidant activity from bamboo (Bambusa vulgaris) shoot. The fiber hydrolysate from bamboo shoot (FHBS) was prepared by stepwise enzymatic hydrolysis including amylase (1%, w/w), cellulase (1, 2, 3%, w/w) and papain (1%, w/w). The released fiber yield of FHBS increased with increasing cellulose levels in dose dependent manner (P≤ 0.05). It was found that the process with 1% (w/w) amylase for 1 h and 3% (w/w) cellulase for 3 h followed by 1% (w/w) papain for 1 h at 50 ºC, rendered the highest released fiber yield (92.10±1.10%). The resultant FHBS contained 5.76±0.21 % of total dietary fiber with total sugar and reducing sugar contents of 1431.22±46.01 and 918.91±10.57 mg/g solid, respectively. The FHBS exhibited antioxidant activities including ABTS radical scavenging activities (ABTS), DPPH radical scavenging activities (DPPH), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Therefore, FHBS with antioxidant activities could be effectively prepared by using enzymatic hydrolysis and suitable to apply in the fiber fortified products.


 


Keywords: bamboo shoot; fiber hydrolysate; antioxidative properties; enzymatic hydrolysis


*Corresponding author: Tel.: +66 2329 8000 ext 7253 Fax: +66 2329 8527


          E-mail: [email protected]

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References

Dhingra, D., Michael, M., Rajput, H. and Patil, R.T., 2012. Dietary fibre in foods: a review. Journal of Food Science and Technology, 49, 255-266.

Tungland, B.C. and Meyer, D., 2002. Nondigestible oligo and polysaccharides (dietary fibre): their physiology and role in human health and food. Comprehensive Reviews in Food Science and Food Safety, 1, 73-92.

Yang, Y.-Y., Ma, S., Wang, X.-X. and Zheng, X.-L., 2017. Modification and application of dietary fiber in foods. Journal of Chemistry, 2017, 1-8. http://doi.org/10.1155/2017/9340427

Prosky, L., 2000. When is dietary fiber considered a functional food?. BioFactors, 12, 289-297.

Benjakul, S, Chantakun, K. and Karnjanapratum, S., 2018. Impact of retort process on characteristics and bioactivities of herbal soup based on hydrolyzed collagen from seabass skin. Journal of Food Science and Technology, 55, 3779-3791.

Karnjanapratum, S. and Benjakul, S., 2017. Antioxidative and sensory properties of instant coffee fortified with galactose-fish skin gelatin hydrolysate Maillard reaction products. Carpathian Journal of Food Science and Technolog, 9 (1), 90-99.

Thongsombat, W., Sirichote, A. and Chanthachum, S., 2007. The production of guava juice fortified with dietary fiber. Songklanakarin Journal of Science and Technology, 29, 187-196.

Zha, X.-Q., Wang, J.-H., Yang, X.-F., Liang, H., Zhao, L.-L., Bao, S.H., Luo, J.-P., Xu, Y.-Y. and Zhou, B.-B., 2009. Antioxidant properties of polysaccharide fraction with different molecular mass extracted with hot-water from rice bran. Carbohydrate Polymers, 78, 570-575.

Judprasong, K., Charoenkiatkul, S., Sungpuag, P., Vasanachitt, K. and Nakjamanong,Y., 2006. Total and soluble oxalate contents in Thai vegetables, cereal grains and legume seeds and their changes after cooking. Journal of Food Composition and Analysis, 19(4), 340-347.

Pandey, A.K. and Ojha, V., 2014. Precooking processing of bamboo shoots for removal of anti-nutrients. Journal of Food Science and Technology, 51(1), 43-50.

He, M.-X., Wang, J.-L.,Qin, H., Shui, Z., Zhu, Q. and Wu, B., 2014. Bamboo: a new source of carbohydrate for biorefinery. Carbohydrate Polymers, 111(20), 645-654.

Xiao-bing, H., Ji-hua, L., Shao-dan, P. and Li-Jing, L., 2015. Effect of preparation methods on the composition and structure of dietary fiber from bamboo shoot. International Conference on Chemical, Material and Food Engineering, Kunming, Yunnan, China, July 25-26, 2015, 182-186. doi: 10.2991/cmfe-15.2015.43.

Song, Y., Su, W. and Mu, Y.C., 2018. Modification of bamboo shoot dietary fiber by extrusion-cellulase technology and its properties. International Journal of Food Properties, 21, 1219-1232.

Konovalova, V., Guzikevich, K., Burban, A, Kujawski, W., Jarzynka, K. and Kujawa, J., 2016. Enhanced starch hydrolysis using α-amylase immobilized on cellulose ultrafiltration affinity membrane. Carbohydrate Polymers, 152, 710-717.

Wang, C.-H., Ma, Y.-L., Zhu, D.-Y., Wang, H., Ren, Y.-F. and Zhang, J.-G., 2017. Physicochemical and functional properties of dietary fiber from bamboo shoot (Phyllostachys praecox). Emirates Journal of Food and Agriculture, 29(7), 509-517.

Ullah, I., Yin, T., Xiong, S., Zhang, J., Din, Z.-U., 2017. Structural characteristics and physicochemical properties of okara (soybean residue) insoluble dietary fiber modified by high-energy wet media milling. LWT-Food Science and Technology, 82, 15-22.

AOAC, 2002. Official Methods of Analysis. 16thed. Washington DC: Association of Official Analytical Chemists.

Ellefson, W., 1993. Provision of the nutrition labelling and education act. In: D.M. Sullivan and D.E. Carpenter, eds. Methods of Analysis for Nutrition Labeling. Virginia: AOAC International.

Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28, 350-356.

Marsden, W.L., Gray, P.P., Nippard, G.J. and Quinlan, M.R., 2006. Evaluation of the DNS method for analysing lignocellulosic hydrolysates. Journal of Applied Sciences and Environmental Management, 10, 93-96.

Horwitz, W., 1995. Total, Soluble and Insoluble Dietary Fiber in Foods. 16th ed. Arlington, Virginia: AOAC Official Methods of Analysis.

Binsan, W., Benjakul, S., Visessanguan, W., Roytrakul, S. Tanaka, M. and Kishimura, H., 2008. Antioxidative activity of Mungoong, an extract paste, from the cephalothorax of white shrimp (Litopenaeus vannamei). Food Chemistry, 106(1), 185-193.

Benzie, I.F. and Strain, J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70-76.

Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. and Shahidi, F., 2012. Gelatin hydrolysate from blacktip shark skin prepared using papaya latex enzyme: Antioxidant activity and its potential in model systems. Food Chemistry, 135, 1118-1126.

Steel, R.G.D., Torrie, J.H. and Dicky, D.A., 1980. Principles and Procedures of Statistics: A Biometrical Aproach. New York: McGraw-Hill.

Yeh, A.-I., Huang, Y.-C. and Chen, S.H., 2010. Effect of particle size on the rate of enzymatic hydrolysis of cellulose. Carbohydrate Polymers, 79, 192-199.

Peri, S., Karra, S., Lee, Y.Y. and Karim, M.N., 2007. Modeling intrinsic kinetics of enzymatic cellulsoe hydrolysis. Biotechnology Progress, 23, 626-637.

Shafiei, M., Karimi, K., and Taherzadeh, M.J., 2010. Palm date fibers: analysis and enzymatic hydrolysis. International Journal of Molecular Sciences, 11, 4285-4296.

Nongdam, P. and Tikendra, L., 2014. The nutritional facts of bamboo shoots and their usage as important traditional foods of Northeast India. International Scholarly Research Notices, 2014. http://dx.doi.org/10.1155/2014/679073.

Nakajima, N., Ishihara, K. and Matsuura, Y., 2002. Dietary-fiberdegrading enzymes from a human intestinal Clostridium and their application to oligosaccharide production from nonstarchy polysaccharides using immobilized cells. Applied Microbiology and Biotechnology, 59, 182-189.

Charalampopoulos, D. and Rastall, R.A., 2012. Prebiotics in foods. Current Opinion in Biotechnology, 23, 187-191.

Ramos, S., Moulay, L., Granado-Serrano, A.B., Vilanova, O., Muguerza, B., Goya, L. and Bravo, L., 2008. Hypolipidemic effect in cholesterol-fed rats of a soluble fiber-rich product obtained from cocoa husks. Journal of Agricultural and Food Chemistry, 56, 6985-6993.

Fry S.C., 1989. Cellulases, hemicelluloses and auxin-stimulated growth: a possible relationship. Physiologia Plantarum, 75, 532-536.

Reddy, S.S., and Krishnan, C., 2010. Production of prebiotics and antioxidants as health food supplements from lignocellulosic materials using multi enzymatic hydrolysis. International Journal of Chemical Science, 8(3), S535-S549.

Liu, Z.-J., Wang, Y.-L., Li, Q.-L. and Yang, L., 2018. Improved antimelanogenesis and antioxidant effects of polysaccharide from Cuscuta chinensis Lam seeds after enzymatic hydrolysis. Brazilian Journal of Medical and Biological Research, 51(7), e7256. http:// dx. doi.org/10.1590/1414-431X20187256

Xu, J., Xu, L.L., Zhou, Q.W., Hao, S.X., Zhou, T. and Xie, H.J., 2016. Enhanced in vitro antioxidant activity of polysaccharides from Enteromorpha prolifera by enzymatic degradation. Journal of Food Biochemistry, 40, 275-283.

Chen, B.J., Shi, M.J., Cui, S., Hao, S.X., Hider, R.C. and Zhou, T., 2016. Improved antioxidant and anti-tyrosinase activity of polysaccharide from Sargassum fusiforme by degradation. International Journal of Biological Macromolecule, 92, 715-722.

Zhou, J., Hu, N., Wu, Y.L., Pan, Y.J. and Sun, C.R., 2008. Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme. Journal of Zhejiang University-Science B, 9, 721-727.

Wu, Q., Zheng, C., Ning, Z.X. and Yang, B., 2007. Modification of low molecular weight polysaccharides from Tremella fuciformis and their antioxidant activity in vitro. International Journal of Molecular Sciences, 8, 670-679.

Tabarsa, M., You, S.G., Dbaghian, E.H. and Surayot, U., 2018. Water-soluble polysaccharides from Ulva intestinalis: Molecular properties, structural elucidation and immunomodulatory activities. Journal of Food and Drug Analysis, 26(2), 599-608.

Chen, S.K., Tsai, M.L. Huang, J.R. and Chen, R.H., 2009. In vitro antioxidant activities of low-molecular-weight polysaccharides with various functional groups. Journal of Agricultural and Food Chemistry, 57, 2699-2704.