Assessment of Some Biochemical Parameters and Dielectric Relaxations in β-Thalassemic Children
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Abstract
Thalassemias are considered as a group of inherited disturbances or blood disorders characterized by the formation of abnormal hemoglobin. β-thalassemia is the most familiar type of thalassemia worldwide and in Egypt. β -thalassemia is classified into three types; minor, intermediate and major. Thalassemia minor resembles anemia with mild iron-deficiency, whilst Thalassemia major is the most severe; patients with the major form need regular blood transfusion stay alive. Patients with the intermediate form show mild to moderate anemia and do not need regular blood transfusion therapy. Therefore, it was thought to be of value to compare the three types on the bases of various biochemical parameters and dielectric properties of blood in the frequency range up to 5 MHz. Sixty children with β-thalassemia were involved in the research. They were divided into three equal groups, according to their thalassemia type, in addition to the same group of control. All children were subjected to thorough clinical examination, and the following blood analyses were performed: hemoglobin electrophoresis, complete blood picture “CBC”, serum ferritin and creatinine, several vital enzymes, total/direct bilirubin, albumin, and other dielectric parameters. Significant differences were found between thalassemia blood and the control, especially in the case of thalassemia major blood. Dielectric relaxations were also detected in thalassemia blood, indicating possible changes in the RBC membranes of thalassemia blood due to possible variations of the surface charges on the RBC membranes and the reduction of glycophorin sialic acid content. Therefore, the present work reveals significant variations between the biochemical and dielectric properties of the three types of thalassemia blood and control blood, and these differences may be of value in the treatment of thalassemia in children.
Keywords: β-thalassemia; antioxidant; CBC; liver enzyme; dielectric; relaxations
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E-mail: yasserkhedr2001@yahoo.com
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References
Rund, D. and Rachmilewitz E., 2005. Beta-thalassemia. New England Journal of Medicine, 353, 1135-46.
Muncie, J.H. and Campbell, J., 2009. Alpha and beta thalassemia. American Family Physician, 80(4), 339-344.
Galanello, R. and Origa R., 2010. Beta-thalassemia. Orphanet Journal of Rare Diseases, 5:1-15.
Elbeshlawy, A. and Yossry, I., 2009. Prevention of Haemoglobinopathies in Egypt. Hemoglobin, 33, 4-20.
Musallam, K.M., Taher, A.T. and Rachmilewitz, E.A., 2012. β-thalassemia intermedia: a clinical perspective. Cold Spring Harbor perspectives in medicine, 2(7), a013482. https://doi: 10.1101/cshperspect.a013482
Camaschella, C., 1995. Thalassemia intermedia. Haematologica, 80, 58-68.
Sangkitporn, S., Sangkitporn, S.K., Tanjatham, S., Suwannakan, B., Rithapirom, S., Yodtup, C. and Duangruang, S., 2011. Multicenter validation of fully automated capillary electrophoresis method for diagnosis of thalassmias and hemoglobinpathies in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 42, 1224-1232.
Bain, B.J., Lewis, S.M. and Bates, I., 2006. Basic haematological techniques. In: Lewis, S.M., Bain, B.J. and Bates I, eds. Dacie and Lewis Practical Haematology. 10th ed. Philadelphia: Churchill Livingstone Elsevier, pp. 40-57.
Ikram, N., Hassan, K., Younas, M. and Amanat, S., 2004. Ferritin levels in patients of beta thalassaemia major. International Journal Pathology, 2, 71-4.
Burtis, C.A., Ashwood, E.R. and Bruns, D.E., 2008. Tietz Fundamentals of Clinical Chemistry. 6th ed. St Louis: Elsevier Saunders Company.
Ohkawa, H., Ohishi, W. and Yagi, K., 2013. Assay for lipid peroxides in animal. European Scientific Journal, 9(24), 351-358.
Habig, W.H., Pabst, M.J. and Jakoby, W.B., 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 249, 7130-7139.
Bonnefont-Rousselot, D., Lehmann, E., Jaudon, M.C., Delattre, J., Perrone, B. and Rechke, J.P., 2000. Blood oxidative stress and lipoprotein oxidizability in haemodialysis patients: effect of the use of a vitamin E-coated dialysis membrane. Nephrology Dialysis Transplantation, 15, 2020-2028.
Goth, L., 1991. A simple method for determination of serum and erythrocyte catalase activity and the revision of the reference range. Clinica Chimica Acta, 196, 143-145.
Marklund, S. and Marklund, G., 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry, 47, 469-474.
Draper, H.H. and Hadley, M., 1990. Malondialdehyde determination an index of liquid peroxidation. Methods in Enzymology, 186, 421-431.
Polk, C. and Postow, C., 1996. Handbook of Biological Effects of Electromagnetic Fields. 2nd ed. London: CRC Press Inc..
Irimajiri, A., Asami, K., Ichinowatari, T. and Kinoshita, Y., 1987. Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. I. Dielectric behavior of cell suspensions in 0.01-500 MHz and its simulation with a single-shell model. Biochimica et Biophysica Acta (BBA)-Biomembranes, 896(2), 203-213.
Asamiero, K., Takahashi, Y. and Takashima, S., 1988. Dielectric properties of mouse lymphocytes and erythrocytes. Biochimica et Biophysica Acta,1010, 49-55.
Ou, Z., Li, Q., Liu, W. and Sun, X., 2011. Elevated hemoglobin A2 as a marker for β-thalassemia trait in pregnant women. The Tohoku Journal of Experimental Medicine, 223(3), 223-226.
Mankad, G.P., Mankad, B. and Singh, S.P., 2013. A study of serological and hematological parameters in thalassaemic patients of Rajkot, Gujarat. International Journal of Scientific and Research Publications, 3,(7), 1-4.
Knovich, M.A., Storey, J.A., Coffman, L.G. and Torti, S.V., 2009. Ferritin for the clinician. Blood Reviews, 23(3), 95-104.
Brittenham, G.M., Patricia, G.M., Arthur, N.W., Christine, M.E. and Young, N.S., 1994. Efficacy of deferoxamine in preventing complications of iron overload in patients with thalassemia major. New England Journal of Medicine, 331(9), 567-573.
Pavlova, L.E., Savov, V.M., Petkov, H.G. and Charova, I.P. 2007. Oxidative stress in patients with beta-thalassemia major. Prilozi, 28(1), 145-154.
Ghone, R.A., Kumbar, K.M., Suryakar, A.N., Katkam, R.V. and Joshi, N.G. 2008. Oxidative stress and disturbance in antioxidant balance in beta thalassemia major. Indian Journal of Clinical Biochemistry, 23(4), 337-340.
Naithanj, R., Chandra, J., Bhattacharjee, J., Verma, P. and Naravan, S. 2006. Peroxidative stress and antioxidant enzymes in children with beta thalassemia major. Paediatric Blood Cancer, 46(7), 780-785.
Percival, M. 1998. Antioxidants. Clinical Nutrition Insights, 31(10), 1-4.
Haemmerich, D., Staelin, S.T., Tsai, J.Z., Tungjitkusolmun, S., Mahvi, D.M. and Webster, J.G. 2003. In vivo electrical conductivity of hepatic tumors. Physiological Measurement, 24, 251-260.
Eylar, E.H., Madoff, M.A., Brody, O.V. and Oncley, J.L. 1962. The contribution of sialic acid to the surface charge of the erythrocyte. Journal of Biological Chemistry, 237, 1992-2000.
Kahane, I., Ben-Chetrit, E., Shifter, A. and Rachmilewitz, E.A. 1980. The erythrocyte membranes in beta-thalassemia. Lower sialic acid levels in glycophorin. Biochimica et Biophysica Acta, 596, 10-17.
Desouky, O.S., Selim, N.S., El-Bakrawy, E.M. and El-Marakby, S.M. 2009. Biophysical characterization of β-thalassemic red blood cells. Cell Biochemistry and Biophysics, 55(1), 45-53.