Micropropagation of Koelreuteria bipinnata Using Juvenile and Mature Explants
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Abstract
An efficient micropropagation protocol for Koelreuteria bipinnata, an ornamental tree, from mature and juvenile phase tissues was described. Nodal explants from one-year-old branches of a field-grown mature tree (mature phase) and from in vitro growing seedlings (juvenile phase) were used. The nodal explants from mature explants cultured on Murashig and Skoog (MS) medium supplemented with or without growth regulators did not show any axillary shoot development. However, 6-benzylaminopurine (BAP) at 1.5 mg/l induced axillary shoots from all juvenile explants (100%). It also produced the highest number of shoots (2.5 shoots per explant), the highest rooting percentage (100%), the maximum number of roots (2.80 roots/shoot) and the longest roots (5.87 cm) when it was added as a supplement to the half-strength MS medium culture that included 15 g/l sucrose. Plantlets were successfully acclimatized and transferred to the field with 60% survival rate.
Keywords: BAP; Chinese flame tree; Juvenile nodal explant; mature nodal explants; sucrose; woody plants
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References
Xia, N. and Gadek, P.A., 2007. Sapindaceae. In: Z.Y. Wu, P.H. Raven and D.Y. Hong, eds. Flora of China. Vol. 12. (Hippocastanaceae through Theaceae). Beijing: Science Press/ Missouri Botanical Garden Press, pp. 5-24.
Gilman, E.F. and Watson, D.G., 1993. Koelreuteria bipinnata Chinese Flame-Tree. [online] Available at https://hort.ifas.ufl.edu/database/documents/pdf/tree_fact_sheets/koebipa.pdf
Urban Forest Ecosystem Institute, 2020. SelecTree. "Koelreuteria bipinnata Tree Record." 1995-2020. [online] Available at https://selectree.calpoly.edu/tree-detail/koelreuteria-elegans
Cook, A., Turner, S.R., Baskin, J.M., Baskin, C.C., Steadman, K.J. and Dixon, K.W., 2008. Occurrence of physical dormancy in seeds of Australian Sapindaceae: a survey of 14 species in nine genera. Annals of Botany, 101(9), 1349-1362.
Park, I.-H. and Rehman, S., 1999. Studies on seed dormancy: seeds maturation in relation to dormancy in golden rain tree (Koelreuteria paniculata Laxm.). Acta Horticulturae, 504(21), 199-208.
Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L. 2009. Propagation of ornamental trees, shrubs, and woody vines. In: H.T. Hartmann, D.E. Kester, F.T. Davies and R.L. Geneve. Hartmann and Kester's Plant Propagation Principles and Practices. Harlow: Pearson Education Limited, pp. 774-839.
Bonga, J.M., 1987. Clonal propagation of mature trees: problems and possible solutions. In: J.M. Bonga and D.J. Durzan, eds. Cell and Tissue Culture in Forestry. Dordrecht: Springer, pp. 249-271.
Hackett, W.P. and Murray, J.R., 1993. Maturation and rejuvenation in woody species. In: M.R. Ahuja, ed. Micropropagation of Woody Plants. Dordrecht: Springer, pp. 93-105.
Feng, D.-L., Meng, Q.-R., Li, W.-P., Hu, Y.-H., Li, M. and Gu, A.-X., 2010. Morphology of somatic embryogenesis and plantlet formation in tissue cultures of lantern tree (Koelreuteria bipinnata var. integrifoliola). Forestry Studies in China, 12(1), 31-36.
Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
Gomez, K.A. and Gomez, A.A., 1984. Statistical Procedures for Agricultural Research. 2nd ed. New York: JohnWiley.
Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L., 2011. Plant Propagation: Principles and Practices. 8th ed. New Jersey: Prentice-Hall.
Andersone, U. and Ievinsh, G., 2002. Changes of morphogenic competence in mature Pinus sylvestris L. buds in vitro. Annals of Botany, 90(2), 293-298.
Borthakur, A., Das, S.C., Kalita, M.C. and Sen, P., 2011. In vitro plant regeneration from apical buds of Albizzia odoratissima (L.f.) Benth. Advances Applied Science Research, 2, 457-464.
Hung, C.D. and Trueman, S.J., 2011. In vitro propagation of the African mahogany Khaya senegalensis. New Forests, 42(1), 117-130.
Jones, A.M.P. and Saxena, P.K., 2013. Inhibition of phenylpropanoid biosynthesis in Artemisia annua L.: a novel approach to reduce oxidative browning in plant tissue culture. PloS One, 8(10), e76802, https://doi.org/10.1371/journal.pone.0076802
Kieber, J.J. and Schaller, G.E., 2014. Cytokinins. The Arabidopsis Book, 12, https://doi.org/ 10.1199/tab.0168.
Tawfik, A.A. and Read P.E., 1991. Effect of benzyladenine on the oil constituents of rosemary plants regenerated from callus. Plant Growth Regulator Society of America, 37-39.
Tawfik, A.A., 1997. Micropropagation and plant regeneration of neem tree (Azadirachta indica Juss). Assiut Journal of Agriculture Science, 28(2), 3-14.
van Staden, J., Zazimalova, E. and George, E.F., 2008. Plant growth regulators II: cytokinins, their analogues and antagonists. In: E.F. George, M.A. Hall and G.-J. De Klerk, eds. Plant Propagation by Tissue Culture. Vol 1. The Background. 3rd ed. Dordrecht : Springer, pp. 205-226
Huetteman, C.A. and Preece, J.E., 1993. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33(2), 105-119.
Tawfik, A.A., Read, P.E. and Cuppett, S.L., 1998. Rosmarinus officinalis L. (Rosemary): In vitro culture, regeneration of plants, and the level of essential oil and monoterpenoid constituents. In: Y.P.S. Bajaj, ed. Medicinal and Aromatic Plants X. Biotechnology in Agriculture and Forestry Vol. 41. Berlin: Springer-Verlag, pp. 349-365.
Tawfik, A.A., 2002. Adventitious meristem organogenesis and shoot proliferation of Salvia officinalis L. in split-nodal culture. Assiut Journal of Agriculture Science, 33(2), 99-114.
Tawfik, A.A. and Mohamed, M.F., 2006. Shoot differentiation and plant regeneration from thidiazuron-induced callus of Salvia officinalis. Acta Horticulture, 723, 309-313.
Wang, Y. and Yao, R., 2017. Plantlet regeneration of adult Pinus massoniana Lamb. Trees using explants collected in March and thidiazuron in culture medium. Journal of Forestry Research, 28(6), 1169-1175.
Groach, R., Dar, M.H., Badgal, K.C., Pal, P., Singh, N. and Yadav, K.U.M., 2014. Plantlet regeneration through indirect organogenesis of flame gold tree (Koelreuteria elegans Laxm.). Journal of Ornamental Plants, 4(3), 175-180.
Yang, X., Yang, X., Guo, T., Gao, K., Zhao, T., Chen, Z. and An, X., 2018. High-efficiency somatic embryogenesis from seedlings of Koelreuteria paniculata Laxm. Forests, 9(12), 769, https://doi.org/10.3390/f9120769
Tawfik, A.A., 1995. In vitro propagation of Melaleuca armillaris. Assiut Journal of Agriculture Science, 26(4),137-149.
Tawfik, A.A., Ibrahim, O.H.M., Abdul-Hafeez, E.Y. and Ibrahim, S.A., 2018. Optimizing micropropagation protocol for Rosa hybrida cv. Eiffel Tower with improved in vitro rooting ability. Egypt Journal of Horticulture, 45(2), 323-335.