Identification and Plant Growth-Promoting Activities of Proteobacteria Isolated from Root Nodules and Rhizospheric Soils
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Abstract
Phosphate solubilization, zinc solubilization, nitrogen fixation and indole-3-acetic acid (IAA) production are plant growth-promoting activities that occur in certain kinds of bacteria. In particular, the rod-shaped proteobacteria isolated from root nodules and rhizospheric soil are considered to be plant growth-promoting. This study aimed to screen and identify plant growth-producing bacteria and included a study of the IAA optimization of the selected isolates. A total of twelve Gram-negative, rod-shaped bacteria were isolated from Leguminosae root nodules and Leguminosae rhizospheric soils. The samples were collected in Udon Thani, Nong Bua Lumphu and Nakhon Phanom provinces, Thailand. Based on the phenotypic characteristics and 16S rRNA gene sequence similarities (99.2-100% similarity), the isolates were characterized as relatives of Rhizobium pusense (2 isolates), Ochrobactrum oryzae (1 isolate), Pseudomonas aeruginosa (1 isolate), Acinetobacter pittii (1 isolate), Klebsiella pneumoniae subsp. rhinoscleromatis (1 isolate), and Pseudomonas geniculata (6 isolates). The isolates were screened for their plant-growth-promoting activities. The results revealed that 8 isolates exhibited phosphate solubilization (10.9 ± 0.00 to 17.0 ± 1.40 Solubilization Index, SI), zinc solubilization (21.5 ± 0.70 to 33.0 ± 1.40 SI) and indole-3-acetic acid production (1.0 ± 0.2 to 113.4±3.5 µg/ml), and 2 isolates showed their nitrogen-fixing activities. The isolates SN1, SN3-3, SN5, PN1-1 and LS1 were selected and optimized for IAA production.
Keywords: proteobacteria; phosphate solubilizing; zinc solubilizing; indole-3-acetic acid
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References
Kuklinsky-Sobral, J., Araujo, W., Mendes, R., Geraldi, I., Pizzirani-Kleiner, A. and Azevedo, J., 2004. Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion. Environmental Microbiology, 6, 1244-1251.
Blaha, D., Prigent-Combaret, C., Mirza, M.S. and Moënne-Loccoz, Y., 2006. Phylogeny of the 1 aminocyclopropane-1-carboxylic acid deaminase-encoding gene acdS in phytobeneficial and pathogenic proteobacteria and relation with strain biogeography. FEMS Microbiology Ecology, 56, 455-470.
Shahzad, F., Shafee, M., Abbas, F., Babar, S., Tariq, M.M. and Ahmed, Z., 2012. Isolation and biochemical characterization of Rhizobium meliloti from root nodules of Alfalfa (Medica sativa). Journal of Animal and Plant Sciences, 22, 522-524.
Chen, Y.P., Rekha, P.D., Arun, A.B., Shen, F.T., Lai, W.A. and Young, C.C., 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology, 34, 33-41.
Saravanan, V.S., Madhaiyan, M. and Thangaraju, M., 2007. Solubilization of zinc compounds by the diazotrophic, plant growth promoting bacterium Gluconacetobacter diazotrophicus. Chemosphere, 66, 1794-1798.
Lynch, J.M., 1985. Origin, nature and biological activity of aliphatic substances and growth hormones found in soil. In: D. Vaughan and R.E. Malcom, eds. Soil Organic Matter and Biological Activity. Dordrecht, Boston, Lancaster: Martinus Nijhoff/Dr. W. Junk Publishers, pp.151-174.
Riker, A.J. and Riker, R.S., 1936. Introduction to Research on Plant Diseases. A Guide to the Principles and Practice for Studying Various Plant-disease Problems. St. Louis, Chicago, New York and Indianapolis: John’s Swift Co.
Barrow, G.I. and Feltham, R.K.A., 1993. Cowan and Steel’s Manual for the Identification of Medical Bacteria. New York: Cambridge University Press.
Tanasupawat, S., Okada, S. and Komagata, K., 1998. Lactic acid bacteria found in fermented fish in Thailand. Journal of General and Applied Microbiology, 44, 193-200.
Lane, D. J., 1991.16S/23S rRNA sequencing. In: E. Stackebrandt and M. Goodfellow, eds. Nucleic Acid Techniques in Bacterial Systematics. New York: John Wiley & Sons, pp.115-175.
Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H. and Won, S., 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, 716-721.
Saitou, N. and Nei, M., 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406-425.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S., 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729.
Felsenstein, J., 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783-791.
Tariq, S., Amin, A. and Latif, Z., 2015. PCR based DNA fingerprinting of mercury resistant and nitrogen fixing Pseudomonas spp. Pure and Applied Biology, 4, 129-136.
Pikovskaya, R.I., 1948. Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiology, 17, 362-370.
Nautiyal, C.S., 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, 170, 265-270.
Vogel, A.I. and Svehla, G., 1979. Vogel’s Textbook of Macro and Semimicro Qualitative Inorganic Analysis. London: Longman.
Ehmann, A., 1977. The Van Urk-Salkowski reagent-a sensitive and specific chromogenic reagent for silica. Journal of Chromatography A, 132, 267-276.
Vaghasiat, H.L., Patel, G.M., Chudasama, R.S. and Bhott, K.R., 2011. Screening of IAA from rhizosphere microflora of field crops. Bioscience Discovery, 2, 94-100.
Chibani, H.R., Bouznad, A., Bellahcene, M. and Djibaoui, R., 2017. Screening and characterization of plant growth promoting traits of phosphate solubilizing bacteria isolated from wheat rhizosphere of Algerian saline soil. Malaysian Journal of Microbiology, 13, 124-131.
Mohite, B., 2013. Isolation and characterization of indole-3-acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of Soil Science and Plant Nutrition, 13, 638-649.
Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11, 1-42.
Panday, D., Schumann, P. and Das, K.S., 2011. Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.). International Journal of Systematic and Evolutionary Microbiology, 61, 2632-2639.
Tripathi, K.A., Verma, C.S., Chowdhury, P.S., Lebuhn, M., Gattinger, A. and Schloter, M., 2006. Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India. International Journal of Systematic and Evolutionary Microbiology, 56, 1677-1680.
Kersters, K., Ludwig, W., Vancanneyt, M., De Vos, P., Gillis, M. and Schleifer, K-H., 1996. Recent changes in the classification of the pseudomonads: an overview. Systematic and Applied Microbiology, 19, 465-477.
Anzai, Y., Kim, H., Park, J.Y., Wakabayashi, H. and Oyaizu, H., 2000. Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. International Journal of Systematic and Evolutionary Microbiology, 50, 1563-1589.
Chang, C.H., Wei, F.U., Dijkshoorn, L., Vaneechoutte, M., Tang, T.C. and Chang, C.T., 2005. Species-Level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region. Journal of Clinical Microbiology, 43, 1632-1639.
Corelli, B., Almeida, S., Sonego, F., Castiglia, V., Fevre, C., Brisse, S., Sansonetti, J.P. and Tournebize, R., 2018. Rhinoscleroma pathogenesis: The type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formation. PLoS Neglected Tropical Diseases, 12, e0006201, https://doi.org./10.1371/journal.pntd.0006201.
Costacurta, A. and Vanderleyden, J., 1995. Synthesis of phytohormones by plant associated bacteria. Critical Reviews in Microbiology, 21, 1-18.
Sudha, M., Shyamala, G.R., Prbhavati, P., Astapritya, P., Yamuna Devi, Y. and Saranya, A., 2012. Production and optimization of Indole-3-acetic acid by indigenous microflora using agro waste as substrate. Pakistan Journal of Biological Sciences, 15, 39-43.
Mandal, S.M., Mondal, K.C., Dey, S. and Pati, B.R., 2007. Optimization of cultural and nutritional conditions for indole-3-acetic acid (IAA) production by a Rhizobium sp. isolated from root nodules of Vigna mungo (L.) Hepper. Research Journal of Microbiology, 2, 239-246.
Khamna, S., Yokota, A., Peberdy, J.F. and Lumyong, S., 2010. Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. EurAsian Journal of BioSciences, 4, 23-32.
Acuña, J.J., Jorquera, M.A., Martínez, O.A., Menezes-Blackburn, D., Fernández, M.T., Marschner, P., Greiner, R. and Mora, M.L., 2011. Indole-3-acetic acid and phytase activity produced by rhizosphere bacilli as affected by pH and metals. Journal of Soil Science and Plant Nutrition, 11, 1-12.
Lindow, E., Desurmont, C., Elkins, R., Mccourty, G., Clark, E. and Maria, T.B., 1998. Occurrence of Indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet. Phytophathology, 88, 1149-1157.
Datta, C. and Basu, P., 2000. lndole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub Cajanus cojan. Microbiological Research, 155, 123-127.
Matsukawa, E., Nakagawa, Y., Iimura, Y. and Hayakawa, M., 2007. Stimulatory effect of indole-3-acetic acid on aerial mycelium formation and antibiotic production in Streptomyces spp. Actinomycetologica, 21, 32-39.
Sarwar, M. and Kremer, R.J., 1992. Determination of bacterially derived auxins using a microplate method. Letters in Applied Microbiology, 20, 282-285.