Semi-field Application of a New Formulation Based on Spodoptera litura Nuclear Polyhedrosis Virus and Bacillus thuringiensis subsp. mexicanensis Against Cotton Leaf Worm, Spodoptera littoralis and Root Knot Nematode, Meloidogyne incognita in Egypt
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Abstract
In this study, new biopreparations based on Spodoptera litura nuclear polyhedrosis virus (SlituraNPV) and Bacillus thuringiensis subsp. mexicanensis (Btm) were evaluated against two important pests: cotton leaf worm, Spodoptera littoralis, and the root knot nematode, Meloidogyne incognita, in 2017 and 2018. The mortality of treated S. littoralis larvae occurred, with combination treatments of SlituraNPV and Btm at two concentrations (20 and 30%), without significant differences between the two tested concentrations (87.9 and 94.0, respectively). Deformities among pupae were the highest in Btm treatments, followed by SlituraNPV treatments, then the fewest deformed pupae were recorded in the combined treatment. Increases of the soybean mean seed weights were highly significant with combined treatment when compared to the controls, in both years, and the greatest expansion was seen with combination treatment. The treatment of M. incognita with Btm was highly effective for nematode J2-mortality. Furthermore, the mean numbers of all recorded parameters for tomato showed significant differences.
Keywords: nuclear polyhedrosis virus; bacteria; cotton leaf worm; nematode; field application
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