Mass Propagation System of Strawberry (Fragaria × ananassa) Microshoots by Liquid Shake Culture
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Abstract
The future of strawberry propagation and sales is very positive, so an efficient mass propagation system for strawberry (Fragaria × ananassa) was developed in this study. Strawberry leaf explants successfully induced green compact calli in semi-solid MS medium supplemented with 1 mg/l 2,4-D and 0.5 mg/l BA within 2 weeks. Subculture the calli in semi-solid MS medium supplemented with 1 mg/l TDZ and 0.2 mg/l 2,4-D produced 3.8 microshoots per callus within 49-56 days. These leaf-derived calli with adventitious shoots were cultured with 0.1 mg/l and shaken at 120 rpm. The number of shoots increased from 15.3 shoots to 43.6 shoots per culture (100 ml) within 2 weeks, but all were hyperhydric shoots. Hyperhydric shoots recovered to normal shoots within 1 month when cultured on semi-solid MS medium with 8 g/l agar. Shoots formed roots on semi-solid MS medium supplemented with 0.5 mg/l IBA within 2 weeks. Rooted plantlets were acclimatized for 1 week before being transferred to the field at Bendito farm, where the strawberry plants were grown until maturity.
Keywords: hyperhydric; liquid shake culture; mass propagation; microshoots; strawberry
*Corresponding author: E-mail: [email protected]
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References
de Souza, V.R., Pereira, P.A., da Silva, T.L., de Oliveira L.L.C., Pio, R. and Queiroz, F., 2014. Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits. Food Chemistry, 156, 362-368.
Amatori, S., Mazzoni, L., Alvarez-Suarez, J.M., Giampieri, F., Gasparrini, M., Forbes-Hernandez, T.Y., Afrin, S., Provenzano, A.E., Persico, G., Mezzetti, B., Amici, A., Fanelli, M. and Battino, M., 2016. Polyphenol-rich strawberry extract (PRSE) shows in vitro and in vivo biological activity against invasive breast cancer cells. Scientific Reports, 6, 30917, https://doi.org/10.1038/srep30917.
Donnoli, R., Sunseri, F., Martelli, G. and Greco, I., 2001. Somatic embryogenesis, plant regeneration and genetictransformation in Fragaria spp. Acta Horticulturae, 560, 235-240.
Ramirez-Malagon, R., Aguilar-Ramirez, I., Borodanenko, A., Perez-Moreno, L., Barrera-Guerra, J.L., Nuñez-Palenius, H.G. and Ochoa-Alejo, N., 2007. In vitro propagation of ten threatened species of Mammillaria (Cactaceae). In Vitro Cellular and Developmental Biology-Plant, 43, 660-665.
Martin, K.P., Joseph, D., Madassery, J. and Philip, V.J., 2003. Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andreanum. In Vitro Cellular and Developmental Biology-Plant, 39, 500-504.
Hasan, M.N., Nigar, S., Rabbi, M.A.K., Mizan, S.B. and Rahman, M.S., 2010. Micropropagation of strawberry (Fragaria x ananassa Duch.). International Journal of Sustainable Crop Production, 5(4), 36-41.
Miller, A.R. and Chandler C.K., 1990. Plant regeneration from excised cotyledons of mature strawberry achenes. HortScience, 25(5), 569-571.
Nehra, N.S., Stushnoff, C. and Kartha, K.K., 1990. Regeneration of plants from immature leaf-derived callus of strawberry (Fragaria x ananassa). Plant Science, 66(1), 119-126.
Liu, Z.R. and Sanford, J.C., 1988. Plant regeneration by organogenesis from strawberry leaf and runner culture. HortScience, 23, 1056-1059.
Nehra, N.S., Stushnoff, C. and Kartha, K.K., 1989. Direct shoot regeneration from strawberry leaf disks. Journal of the American Society for Horticultural Science, 114, 1014-1018.
James, D.J., Passey, A.J. and Barbara, D.J., 1990. Agrobacterium-mediated transformation of the cultivated strawberry (Fragaria x ananassa Duch.) using disarmed binary vectors. Plant Science, 69(1), 79-94.
Șuțan, A.N., Popescu, A. and Isac, V., 2010. In vitro culture medium and explant type effect on callogenesis and shoot regeneration in two genotypes of ornamental strawberry. Romanian Biotechnological Letters, 15(2), 12-17.
Moradi, K.., Otroshy, M. and Azimi, M.R., 2011. Micropropagation of strawberry by multiple shoots regeneration tissue cultures. Journal of Agricultural Technology, 7(6), 1755-1763.
Folta, K.M., Dhingra, A., Howard, L., Stewart, P.J. and Chandler, C.K., 2006. Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation. Planta, 224, 1058-1067.
Ara, T., Karim, R., Karim, M.R., Islam, R. and Hossain, M., 2012. Callus induction and shoot regeneration in strawberry (Fragaria x ananassa Duch.). International Journal of Biosciences, 2, 93-100.
Debnath, S.C., 2006. Zeatin overcomes thidiazuroninduced inhibition of shoot elongation and promotes rooting in strawberry culture in vitro. The Journal of Horticultural Science and Biotechnology. 81, 349-354.
Preece, J.E., 2010. Micropropagation in stationary liquid media. Propagation of Ornamental plants, 10(4), 183-187.
Grigoriadou, K., Vasilakakis, M., Tzoulis, T. and Eleftheriou, E., 2005. Experimental use of a novel temporary immersion system for liquid culture of olive microshoots. In: A.K. Hvoslef-Eide and W. Preil, eds. Liquid Culture Systems for in vitro Plant Propagation. Dordrecht: Springer, pp. 263-274.
Nhut, D.T., Teixeira da Silva, J.A., Huyena, P.X. and Paek, K.Y., 2004. The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture. Scientia Horticulturae, 102(4), 407-414.
Murashige, T. and Skoog, F., 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15(3), 473-497.
Ciurzyńska, A., Lenart, A. and Karwosińska, J., 2015. Effect of quantity of low-methoxyl pectin on physical properties of freeze-dried strawberry jellies. Polish Journal of Food and Nutrition Sciences, 65(4), 233-241.
Cleland, R.E., 2010. Auxin and cell elongation. In: P.J. Davies, ed. Plant Hormones Biosynthesis, Signal Transduction, Action! 3rd ed. Dordrecht: Springer, pp. 204-220.
George, E.F., Hall, M.A. and Klerk, G.J.D., 2008. Plant growth regulators I: Introduction; auxins, their analogues and inhibitors. In: E.F George, M.A. Hall and G.J.D. Klerk, eds. Plant Propagation by Tissue Culture. 3rd ed. Dordrecht: Springer, pp. 175-204.
Santos, M.R.A., Souza, C.A. and Paz, E.S., 2017. Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño. Revista Ciência Agronômica, 48(3), https://doi.org/10.5935/1806-6690.20170061.
Palei, S., Rout, G.R., Das, D.K. and Dash, D.K., 2017. Callus induction and indirect regeneration of strawberry (Fragaria × Ananassa) Duch. CV. Chandler. International Journal of Current Microbiology and Applied Sciences, 6(11), 1311-1318.
Kurmi, U.S., Sharma, D.K., Tripathi, M.K., Tiwari, R., Baghel, B.S. and Tiwari, S., 2011. Plant regeneration of Vitis vinifera (L) via direct and indirect organogenesis from cultured nodal segments. Journal of Agricultural Technology, 7(3), 721-737.
Diengngan, S. and Murthy, B.N.S., 2014. Influence of plant growth promoting substances in micropropagation of Strawberry cv. Festival. The Bioscan, 9(4), 1491-1493.
Debnath, S.C., 2005. Strawberry sepal: Another explant for thidiazuron-induced adventitious shoot regeneration. In Vitro Cellular and Developmental Biology-Plant, 41, 671-676.
Passey, A.J., Barrett, KJ. and James, D.J., 2003. Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria x ananassa Duch.) using a range of explant types. Plant Cell Reports, 21, 397-401.
Litwinczuk, W., Okolotkiewicz, E. and Matyaszek, I., 2009. Development of in vitro shoot cultures of strawberry (Fragaria x ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid. Floria Horticulturae, 21(2), 43-52.
Zatyko, J.M., Kiss, G., Radics, ZS. and Simon, I., 1989. Initiation of strawberry runner formation in vitro. Acta Horticulturae, 265, 349-352.
Valles, M. and Boxus, P.H., 1987. Micropropagation of several Rosa hybrida L. cultivars. Acta Horticulturae, 212, 611-617.
Barbosa, L., Neto, V.B.P., Dias, L.C., Festucci-Buselli, R.A., Alexandre, R.S., Iarema, L., Finger, F.L. and Otoni, W.C., 2013. Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents. Revista Ceres, 60(2), 43-52.
Casanova, E., Moysset, L. and Trillas, M.I., 2008. Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots. Biologia Plantarum, 52(1), 1-8.