Development of New Functional Beverage: Longan Kefir Supplemented with Herbs
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Abstract
In recent years, the increasing awareness of diseases has led to the demand for functional foods and beverages that provide additional health benefits beyond basic nutrition. Water kefir is a healthy fermented beverage with unique aroma and flavor profile, obtained by the symbiotic fermentation of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeast. In this study, a new functional beverage based on substrates such as longan juice (KL) and longan juice supplemented with herbs: butterfly pea (KLB), rosella (KLR), or safflower (KLS), and fermented at 25°C for 48 h with water kefir grains as starter, was developed. The occurrence of microbial community of LAB, AAB and yeast during fermentation in all treatments were not significantly different (p<0.05) However, each supplement showed its distinct color according to specific characters of the herbs. The addition of the herbs significantly enhanced total phenolic compounds (TPCs) and antioxidant activities as investigated by DPPH and ABTS assay, with the results varying with supplement type. The addition of KLS showed the highest TPC of 212.17 μgGAE/mL, while KLB and KLR displayed lower levels of 198.36 and 201.93 μgGAE/mL, respectively. Levels of DPPH and ABTS in all supplements showed the best condition at 24 h fermentation, but decreased at 48 h, suggesting the sensitivity of some antioxidants. Sensory evaluation revealed that KLS had the highest acceptance, while KLR was the least accepted by consumers. This study pointed out that longan kefir supplemented with herbal ingredients has the potential to be a novel functional beverage.
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References
Guzel-Seydim, Z.B., Gökırmaklı, Ç. and Greene, A.K., 2021. A comparison of milk kefir and water kefir: Physical, chemical, microbiological and functional properties. Trends in Food Science and Technology, 113, 42-53.
Moretti, A.F., Moure, M.C., Quiñoy, F., Esposito, F., Simonelli, N., Medrano, M. and León-Peláez, Á., 2022. Water kefir, a fermented beverage containing probiotic microorganisms: From ancient and artisanal manufacture to industrialized and regulated commercialization. Future Foods, 5, https://doi.org/10.1016/j.fufo.2022.100123.
Lynch, K.M., Wilkinson, S., Daenen, L. and Arendt, E.K., 2021. An update on water kefir: Microbiology, composition and production. International Journal of Food Microbiology, 345, https://doi.org/10.1016/j.ijfoodmicro.2021.109128.
Cai, Y., Sounderrajan, A. and Serventi, L., 2020. Water kefir: A review of its microbiological profile, antioxidant potential and sensory quality. Acta Scientific Nutritional Health, 4(6), 10-17.
Shao, N., 2022. Riding on waves and filling the cracks: Toward an inclusive and sustainable Sino-Thai longan supply chain management in upper Thailand. ASR Chiang Mai University Journal of Social Sciences and Humanities, 9(2), 1-17.
Zhang, X., Guo, S., Ho, C.-T. and Bai, N., 2020. Phytochemical constituents and biological activities of longan (Dimocarpus longan Lour.) fruit: A review. Food Science and Human Wellness, 9(2), 95-102.
Yang, B., Jiang, Y., Shi, J., Chen, F. and Ashraf, M., 2011. Extraction and pharmacological properties of bioactive compounds from longan (Dimocarpus longan Lour.) fruit—A review. Food Research International, 44(7), 1837-1842.
Lijon, M.B., Meghla, N.S., Jahedi, E., Rahman, M.A. and Hossain, I., 2017. Phytochemistry and pharmacological activities of Clitoria ternatea. International Journal of Natural and Social Sciences, 4(1), 1-10.
Zhang, L.-L., Tian, K., Tang, Z.-H., Chen, X.-J., Bian, Z.-X., Wang, Y.-T. and Lu, J.-J., 2016. Phytochemistry and pharmacology of Carthamus tinctorius L. The American Journal of Chinese Medicine, 44(02), 197-226.
Riaz, G. and Chopra, R., 2018. A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomedicine and Pharmacotherapy, 102, 575-586.
Reed, R.W. and Reed, G.B., 1948. "Drop plate" method of counting viable bacteria. Canadian Journal of Research, 26(6), 317-326.
Klawpiyapamornkun, T., Bovonsombut, S. and Bovonsombut, S., 2015. Isolation and characterization of acetic acid bacteria from fruits and fermented fruit juices for vinegar production. Food and Applied Bioscience Journal, 3(1), 30-38.
Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178.
Xiao, F., Xu, T., Lu, B. and Liu, R., 2020. Guidelines for antioxidant assays for food components. Food Frontiers, 1(1), 60-69.
AOAC, 2003. Official Method of Analysis. 17th Edition. Washington, D.C.: Association of Analytical.
Hamrita, B., Emira, N., Papetti, A., Badraoui, R., Bouslama, L., Ben Tekfa, M. I., Hamdi, A., Patel, M., Elasbali, A.M., Adnan, M., Sahraf, S.A. and Snoussi, M., 2022. Phytochemical analysis, antioxidant, antimicrobial, and anti-swarming properties of Hibiscus sabdariffa L. calyx extracts: In vitro and in silico modelling approaches. Evidence-Based Complementary and Alternative Medicine, 2022, https://doi.org/10.1155/2022/1252672.
Çömlekcioğlu, N. and Aygan, A., 2020. Fatty acids, bioactive content and antimicrobial activity of Hibiscus sabdariffa L. extract obtained by different techniques. Turkish Journal of Agriculture-Food Science and Technology, 8(12), 2723-2728.
Deorankar, P., Gangiwale, R., Chintamani, R. and Singh, R.P., 2020. Evaluation of ethanolic and aqueous extract of Clitoria ternatea for antimicrobial activity. Indian Journal of Natural Products and Resources, 11(3), 194-198.
Jeyaraj, E.J., Lim, Y.Y. and Choo, W.S., 2021. Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. Journal of Food Science and Technology, 58(6), 2054-2067.
Gulitz, A., Stadie, J., Wenning, M., Ehrmann, M.A. and Vogel, R.F., 2011. The microbial diversity of water kefir. International Journal of Food Microbiology, 151(3), 284-288.
Roy, S. and Rhim, J.W., 2021. Anthocyanin food colorant and its application in pH-responsive color change indicator films. Critical Reviews in Food Science and Nutrition, 61(14), 2297-2325.
Martínez, A., Vegara, S., Herranz‐López, M., Martí, N., Valero, M., Micol, V. and Saura, D., 2017. Kinetic changes of polyphenols, anthocyanins and antioxidant capacity in forced aged hibiscus ale beer. Journal of the Institute of Brewing, 123(1), 58-65.
Amperawati, S., Hastuti, P., Pranoto, Y. and Santoso, U., 2019. The anthocyanins content, color changes and thermal stability of roselle (Hibiscus sabdariffa L.) petal extract. International Journal of Science and Research, 8(4), 428-435.
Kim, H.-J., Roy, S. and Rhim, J.-W., 2022. Gelatin/agar-based color-indicator film integrated with Clitoria ternatea flower anthocyanin and zinc oxide nanoparticles for monitoring freshness of shrimp. Food Hydrocolloids, 124, https://doi.org/10.1016/j.foodhyd.2021.107294.
Kungsuwan, K., Singh, K., Phetkao, S. and Utama-ang, N., 2014. Effects of pH and anthocyanin concentration on color and antioxidant activity of Clitoria ternatea extract. Food and Applied Bioscience Journal, 2(1), 31-46.
Salem, N., Msaada, K., Hamdaoui, G., Limam, F. and Marzouk, B., 2011. Variation in phenolic composition and antioxidant activity during flower development of safflower (Carthamus tinctorius L.). Journal of Agricultural and Food Chemistry, 59(9), 4455-4463.
Jung, Y.J., Assefa, A.D., Lee, J.E., Lee, H.S., Rhee, J.H. and Sung, J.S., 2019. Analysis of antioxidant activity and serotonin derivatives in safflower (Carthamus tinctorius L.) germplasm collected from five countries. Korean Journal of Plant Resources, 32(5), 423-432.
Buyukkurt, O.K., Guclu, G., Barutcular, C., Selli, S. and Kelebek, H., 2021. LC-MS/MS fingerprint and simultaneous quantification of bioactive compounds in safflower petals (Carthamus tinctorius L.). Microchemical Journal, 171, https://doi.org/10.1016/j.microc.2021.106850.
Karimkhani, M.M., Shaddel, R., Khodaparast, M.H.H., Vazirian, M. and Piri-Gheshlaghi, S., 2016. Antioxidant and antibacterial activity of safflower (Carthamus tinctorius L.) extract from four different cultivars. Quality Assurance and Safety of Crops and Foods, 8(4), 565-574.
Borrás-Linares, I., Fernández-Arroyo, S., Arráez-Roman, D., Palmeros-Suárez, P.A., Del Val-Díaz, R., Andrade-Gonzáles, I., Fernández-Gutiérrez, A., Gómez-Leyva, J.F. and Segura-Carretero, A., 2015. Characterization of phenolic compounds, anthocyanidin, antioxidant and antimicrobial activity of 25 varieties of Mexican roselle (Hibiscus sabdariffa). Industrial Crops and Products, 69, 385-394.
Lyu, J.I., Kim, J.M., Kim, D.G., Kim, J.B., Kim, S.H., Ahn, J.W., Kang, S.Y., Ryu, J. and Kwon, S.J., 2020. Phenolic compound content of leaf extracts from different roselle (Hibiscus sabdariffa) accessions. Plant Breeding and Biotechnology, 8(1), 1-10.
Tsai, P.-J., McIntosh, J., Pearce, P., Camden, B. and Jordan, B.R., 2002. Anthocyanin and antioxidant capacity in roselle (Hibiscus sabdariffa L.) extract. Food Research International, 35(4), 351-356.
Escobar‐Ortiz, A., Castaño‐Tostado, E., Rocha‐Guzmán, N.E., Gallegos‐Infante, J.A. and Reynoso‐Camacho, R., 2021. Anthocyanins extraction from Hibiscus sabdariffa and identification of phenolic compounds associated with their stability. Journal of the Science of Food and Agriculture, 101(1), 110-119.
Sabokbar, N. and Khodaiyan, F., 2016. Total phenolic content and antioxidant activities of pomegranate juice and whey based novel beverage fermented by kefir grains. Journal of Food Science and Technology, 53, 739-747.
Hampton, J., Tang, C., Subhash, A.J. and Serventi, L., 2021. Assessment of pear juice and puree as a fermentation matrix for water kefir. Journal of Food Processing and Preservation, 45(3), https://doi.org/10.1111/jfpp.15223.
Rodriguez, H., Curiel, J.A., Landete, J.M., de las Rivas, B., de Felipe, F., Gomez-Cordoves, C., Mancheño, J.M. and Muñoz, R., 2009. Food phenolics and lactic acid bacteria. International Journal of Food Microbiology, 132(2-3), 79-90.
Csapó, J., Prokisch, J., Albert, C. and Sipos, P., 2019. Effect of UV light on food quality and safety. Acta Universitatis Sapientiae, Alimentaria, 12(1), 21-41.
Teixeira, C.C.C., de Freitas Cabral, T.P., Tacon, L.A., Villardi, I.L., Lanchote, A.D. and de Freitas, L.A.P., 2017. Solid state stability of polyphenols from a plant extract after fluid bed atmospheric spray-freeze-drying. Powder Technology, 319, 494-504.
Lu, Z. and Imlay, J.A., 2021. When anaerobes encounter oxygen: mechanisms of oxygen toxicity, tolerance and defence. Nature Reviews Microbiology, 19(12), 774-785.
Rani, A., Saini, K.C., Bast, F., Mehariya, S., Bhatia, S.K., Lavecchia, R. and Zuorro, A., 2021. Microorganisms: A potential source of bioactive molecules for antioxidant applications. Molecules, 26(4), https://doi.org/10.3390/molecules26041142.
Bayliak, M.M., Burdyliuk, N.I. and Lushchak, V.I., 2016. Effects of pH on antioxidant and prooxidant properties of common medicinal herbs. Open Life Sciences, 11(1), 298-307.
Surin, S., Thakeow, P., Seesuriyachan, P., Angeli, S. and Phimolsiripol, Y., 2014. Effect of extraction and concentration processes on properties of longan syrup. Journal of Food Science and Technology, 51(9), 2062-2069.
Laureys, D., Leroy, F., Hauffman, T., Raes, M., Aerts, M., Vandamme, P. and De Vuyst, L., 2021. The type and concentration of inoculum and substrate as well as the presence of oxygen impact the water kefir fermentation process. Frontiers in Microbiology, 12, https://doi.org/10.3389/fmicb.2021.628599.
Verce, M., De Vuyst, L. and Weckx, S., 2019. Shotgun metagenomics of a water kefir fermentation ecosystem reveals a novel Oenococcus species. Frontiers in Microbiology, 10, https://doi.org/10.3389/fmicb.2019.00479.
Indriati, S., Yusuf, M., Riskayanti, R., Amaliah, N., Latief, M., Sjafruddin, R. and Attahmid, N.F.U., 2021. GC-MS and antioxidant capacity analysis in propanol extract of Carthamus tinctorious L. INTEK: Jurnal Penelitian, 8(1), 67-73.
Anggraini, T., Roza, W.W., Sayuti, K. and Asben, N.A., 2022. Potential Clitoria ternatea as colourant for Gambir leaves tea: the antioxidant activity, polyphenols, anthocyanins, catechin, and epigallocatechin gallate. International Journal on Advanced Science, Engineering, and Information Technology, 12(1), 379-384.
Izquierdo-Vega, J.A., Arteaga-Badillo, D.A., Sánchez-Gutiérrez, M., Morales-González, J.A., Vargas-Mendoza, N., Gómez-Aldapa, C.A., Castro-Rosas, J., Delgado-Olivares, L., Madrigal-Bujaidar, E. and Madrigal-Santillán, E., 2020. Organic acids from roselle (Hibiscus sabdariffa L.)- A brief review of its pharmacological effects. Biomedicines, 8(5), https://doi.org/10.3390/biomedicines8050100.