COMPARATIVE STUDY OF REPTILE EGG WHITE PROTEINS BY 2D-PAGE

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Published: Nov 1, 2007
Keywords:
reptile 2D-PAGE egg white protein testudines isoforms

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Sompong Thammasirira*
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
Sakda Daduang
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
Laoo Shawsuan
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand

Abstract

The egg white protein patterns of reptiles have not yet been fully studied. In this study, the egg white proteins of five reptile species were compared with chicken egg white by 2D-PAGE using nonlinear IPG strip pH3-10. The results showed that 35, 26, 45, 15, 33 and 36 protein spots were reproducible detected in Siamese crocodile (Crocodylus siamensis), soft-shelled turtle (Trionyx sinensis tiwanese), red-eared slider turtle (Trachemys scripta elegans), hawksbill turtle  (Eretmochelys imbricate), green sea turtle (Chelonia mydas) and chicken (Gallus gallus) egg white, respectively. After protein pattern analysis, many spots corresponding to major chicken egg white proteins such as ovalbumin and ovotransferrin were found. In egg white of siamese crocodile, 12 isoforms of ovotransferrin like protein were found. The ovalbumin like proteins in reptiles showed different pIs and number of isoforms which are different from chicken egg white. Furthermore, minor proteins, molecular weights lower than 20 kDa were remarkably found in only red-eared slider turtle. The polymorphism of these proteins among reptile species might be served as the different biological function in each species. This study is the first report of proteomic pattern of Testudine egg white proteins. 


Keywords: reptile, 2D-PAGE, egg white protein, testudines, isoforms


Corresponding author: E-mail: somkly@kku.ac.th

Article Details

Issue
Vol. 7 No. 2-1 (2007)
Section
Original Research Articles

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Author Biography

Sompong Thammasirira*, Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand

Department of Biochemistry, Faculty of Science, Khon Kaen University,  Khon Kaen, 40002, Thailand

References

[1] Kovacs-Nolan, J., Phillips, M. and Mine, Y. 2005 Advances in the Value of Eggs and Egg Components for Human Health, Agriculture and Food Chemistry, 53, 8421-8431.
[2] Powrie, W-D. and Nakai, S. 1986 Egg Science and Technology. Macmillan, London, pp. 61-90.
[3] Miguel, M., Manso, M.A., Lopez-Fandino, R. and Ramos, M. 2005 Comparative Study of Egg White Proteins from Different Species by Chromatographic and Electrophoretic Methods, Eur. Food Res Technol., 221, 542-546.
[4] Mine, Y., 1995. Recent Advance in the Understanding of Egg White Protein Functionality, Trends in Food Sci. and Technol., 6, 225-232.
[5] Li-Chan, E. and Nakai, S. 1989 Biochemical Basis for the Properties of Egg White, Crit. Poult. Biol., 2, 21-58.
[6] Kato, A. 1997 Interactions of Egg White Proteins. In: Gaonkar, A., Ed. Ingredients Interactions, Effect on Food Quality. New York, pp. 357-375.
[7] Guerin-Dubiard, C., Pasco, M., Molle, D., Desert, C., Croguennec, T., and Nau, F. 2006 Proteomic Analysis of Hen Egg White, J Agric Food Chem, 54(11), 3901-3910.
[8] Desert, C., Guerin-Dubiard, C., Nau, F., Jan, G., Val, F. and Mallard, J. 2001 Comparison of Different Electrophoretic Separations of Hen Egg White Proteins, Agriculture and Food Chemistry, 49, 4553-4561.
[9] Raikos, V., Hansen, R., Campbell, L. and Euston, S.R. 2006 Separation and Identification of Hen Egg Protein Isoforms using SDS-PAGE and 2D Gel Electrophoresis with MALDI-TOF Mass Spectrometry, Food Chemistry, 99, 702-710.
[10] Nau, F., Pasco, M., Desert, C., Molle, D., Croguennec, T. and Guerin-Dubiard, C. 2005 Identification and Characterization of Ovalbumin Gene Y in Hen Egg White, Agriculture and Food Chemistry, 53, 2158-2163.
[11] Ray, A.K., Guha, M.K. and Sinha, N.K. 1982 Purification and Characterization of an Acidic Trypsin/subtilisin Inhibitor from Tortoise Egg White, Biochimistry Biophysical Acta, 716(2), 126-132.
[12] Chakabarti, S., Sen, P.C. and Sinha, N.K. 1988 Purification and Characterization of a Low Molecular Weight Basic Protein from Marine Turtle Egg White, Archives Biochemistry Biophysical, 262(1), 286-292.
[13] Osada, T., Sasaki, T. and Ikai, 1988 Purification and Characterization of Alpha-Macroglobulin and Ovomacroglobulin of the Green Turtle (Chelonia mydas japonica), Biochemistry, 103(2), 212-217.
[14] Mandal, D.K., Ray, A.K. and Sinha, N.K. 1989 Physical and Chemical Characterization of Macroglobulin from Marine Turtle Egg White, International Journal of Biological Macromolecules, 11(2), 100-104.
[15] Rose, F.L, Paxton, R. and Britton, C. 1990 Identification of an Ovalbumin-like Protein in Egg White of Turtle and Alligator, Comparative Biochemistry and Physiology, 96B, 651-654.
[16] Sil, P.C., Chaudhuri, T.K. and Sinha, N.K. 1993 Basic Trypsin-subtilisin Inhibitor from Marine Turtle Egg White: Hydrodynamic and Inhibitory Properties, Journal of Protein Chemistry, 12, 71-78.
[17] Thammasirirak, S., Ponkham, P., Preecharram, S., Khanchanuan, R., Phonyothee, P., Daduang, S., Srisomsap, C., Araki, T. and Svasti, J. 2006 Purification, Characterization and Comparison of Reptile Lysozymes, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 143(2), 209-217.
[18] Bradford, M. M. 1976 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-dye Binding, Anal. Biochem., 72, 248-254.
[19] O' Farrel, P.H. 1975 High-Resolution Two-dimensional Electrophoresis of Proteins, J. Biol. Chem., 250, 4007-4021.
[20] Görg, A., Postel, W. and Gunther, S. 1988 The Current State of Two-dimensional Electrophoresis with Immobilized pH Gradients, Electrophoresis, 9, 531-546.
[21] Rabilloud, T. 1998 Use of Thiourea to Increase the Solubility of Membrane Proteins in Two-dimensional Electrophoresis, Electrophoresis, 19(5), 758-760.
[22] Görg, A., Postel, W., Weser, J., Gunther, S., Strahler, J.R., Hanash, S.M. and Somerlot, L. 1987 Elimination of Point Streaking on Silver Stained Two-dimensional Gels by Addition of Iodoacetamide to the Equilibration Buffer, Electrophoresis, 8, 122-124.
[23] Laemmli, U.K. 1970 Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, 227 (259), 680-685.
[24] Kang, D., Gho, Y.S., Suh, M. and Kang, C. 2002 Highly Sensitive and Fast Protein Detection with Coomassie Brilliant Blue in Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis, Bull. Korean Chem. Soc., 23(11), 1511-1512.
[25] Peptide Mass Fingerprint
[26] Ciuraskiewiez, J., Olczak, M. and Watorek. 2006 Isolation, Cloning and Sequencing of Transferrins from Red-eared Turtle, African Ostrich, and Turkey, Comparative Biochemistry and Physiology, Part B, 144, 301-310.