COMPARATIVE STUDY OF REPTILE EGG WHITE PROTEINS BY 2D-PAGE
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Abstract
The egg white protein patterns of reptiles have not yet been fully studied. In this study, the egg white proteins of five reptile species were compared with chicken egg white by 2D-PAGE using nonlinear IPG strip pH3-10. The results showed that 35, 26, 45, 15, 33 and 36 protein spots were reproducible detected in Siamese crocodile (Crocodylus siamensis), soft-shelled turtle (Trionyx sinensis tiwanese), red-eared slider turtle (Trachemys scripta elegans), hawksbill turtle (Eretmochelys imbricate), green sea turtle (Chelonia mydas) and chicken (Gallus gallus) egg white, respectively. After protein pattern analysis, many spots corresponding to major chicken egg white proteins such as ovalbumin and ovotransferrin were found. In egg white of siamese crocodile, 12 isoforms of ovotransferrin like protein were found. The ovalbumin like proteins in reptiles showed different pIs and number of isoforms which are different from chicken egg white. Furthermore, minor proteins, molecular weights lower than 20 kDa were remarkably found in only red-eared slider turtle. The polymorphism of these proteins among reptile species might be served as the different biological function in each species. This study is the first report of proteomic pattern of Testudine egg white proteins.
Keywords: reptile, 2D-PAGE, egg white protein, testudines, isoforms
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