Effect of Nucleotides Supplementation on Growth Performance, Humoral Immunity, and Intestinal Morphological Structure of Broiler Chickens

Main Article Content

Tassanee Trairatapiwan
Rachakris Lertpatarakomol
Patiparn Jaipeng
Kornkamon Paditporn


Feeding nucleotides in young animals is believed to have benefit effects such as improving growth rate, enhancing gut health and immune system. Thus, the aim of this present study was to evaluate the effect of nucleotides supplementation in diets at difference periods on growth performance of broiler chickens. A total of 180 Ross 308 (mixed sex), one-day-old broiler chickens were randomly distributed in completely randomized experimental design (CRD) into 3 treatments with 4 replicates of 15 birds in each. The experimental treatments consisted of: birds fed control diet, birds fed 1.5% nucleotides for the first 7 days of age and birds fed 1.5% nucleotides for the first 14 days of age. The dietary period was divided in two phases: starter diet (1 to 21 days) and grower diet (21 to 42 days). The result showed that, at 42 days of ages, there were no significantly difference (P>0.05) in body weight gain, feed intake, feed conversion ratio and HA-HI titer of broiler serum antibodies specific to ND virus between treatments. However, broilers fed with nucleotides had a significant higher (P<0.05) villus height than those fed with control diet. In conclusion, the current study demonstrated that feeding broiler chickens with 1.5%nucleotides in the diet tended to improve growth performance and intestinal morphology of broiler chickens.


Download data is not yet available.

Article Details

Research Article


Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter, P.. 2008. Molecular biology of the cell. 5th ed. New York, NY: Garland Science, Taylor & Francis Group.
Butaye, P., Devriese, L.A. and Haesebrouck, F. 2003. Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on gram-positive bacteria. Clin. Microbiol. Rev. 16(2): 175–188.
Chiofalo, B., Presti, V.L., Savoini, G., D’Alessandro, E., Chiofalo , V. and Liotta., L. 2011. Nucleotides in broiler chicken diet: effect on breast muscle quality. Czech J. Food Sci. 29: 308-317.
Geyra, A., Uni, Z. and Sklan, D. 2001. Enterocyte dynamics and mucosal development in the posthatch chick. Poult. Sci. 80: 776-782.
Hawkes, J.S., Gibson, R.A., Roberton, D. and Makrides, M. 2006. Effect of dietary nucleotide supplementation on growth and immune function in term infants: a randomized controlled trial. Eur. J. Clin. Nutr. 60: 254-264.
Hu, Z. and Guo, Y. 2007. Effects of dietary Sodium butyrate supplementation on the intestinal morphological structure, absorptive function and gut flora in Chickens. Anim. Feed Sci. Technol. 132: 240 - 249.
Jung, B. and Batal, A.B. 2012. Effect of dietary nucleotide supplementation on performance and development of the gastrointestinal tract of broilers. Br. Poult. Sci. 53: 98-105.
Kulkarni, A.D., Rudolph, F.B. and van Buren, C.T. 1994. The role of dietary sources of nucleotides in immune function: a review. J. Nutr. 124: 1442S-1446S.
Mateo, C.D., Peters, D.N. and Stein, H.H. 2004. Nucleotides in sow colostrum and milk at different stages of lactation. J. Anim. Sci. 82: 1339-1342.
Mathews, C.K. and van Holde, K.E. 1990. Biochemistry. Redwood city, CA: The Benjamin/Cummings Publishing Company, Inc.
NRC. 1994. Nutrient Requirements of Poultry: Ninth Revised Edition. Washington, DC: The National Academies Press.
OIE Terrestrial Manual. 2012. Newcastle disease. [Online] Available: http://www.oie.int/fileadmin/Home/fr/Health_standards/tahm/2.03.14_NEWCASTLE_DIS.pdf
Pelicia, V.C., Sartori, J.R., Zavarize, K.C., Pezzato, A.C., Stradiotti, A.C., Araujo, P.C., Mituo, M.A.O. and Madeira, L.A. 2010. Effect of nucleotides on broiler performance and carcass yield. Braz. J. Poult. Sci. 12: 31-34.
Rutz, F., Anciuti, M.A., Rech, J.L., Goncalves, F.M., Delgado, A.D., Rosa, E.R., Zauk, N., Ribeiro, C.L.G. and Da Silva, R.R. 2004. Performance and carcass traits of broilers fed diets containing yeast extract (NuPro®). Proc. Aust. Poult. Symp., Sydney, NSW, Australia: 77-79.
Rutz, F., Xavier, E.G., Anciuti, M.A., Roll, V.F.B. and Rossi, P. 2007. The role of nucleotides in improving broiler prestarter diets: the Brazilian experience. Nutritional biotechnology in the feed and food industries: Proceedings of Alltech's 23rd Annual Symposium. The new energy crisis: food, feed or fuel? 2007 pp. 175-181.
Sauer, N., Bauer, E. and Mosenthin, R. 2009. Dietary nucleotides: potential contenders as feed additive for monogastrics? Proceedings of the 18th international scientific symposium on nutrition of domestic animals. Radenci, Slowenia.
Sauer, N., Eklund, M., Bauer, E., Gänzle, M.G., Field, C.J., Zijlstra, R.T. and Mosenthin, R. 2012. The effects of pure nucleotides on performance, humoral immunity, gut structure and numbers of intestinal bacteria of newly weaned pigs. J. Anim. Sci. 90: 3126-3134.
Uni, Z., Ganot, S. and Sklan, D. 1998. Posthatch development of mucosal function in the broiler small intestine. Poult. Sci. 77: 75-82.
Wang, Z., Cerrate, S., Coto, C., Sacakli, P., Yan, F., Costa, F.G.P. and Waldroup, P.W. 2009. Evaluation of Nupro® yeast product in diets for broilers. Int. J. Poult. Sci. 8: 515-520.