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The experiment was conducted to determine the optimum level of concentration of probiotic supplement, Bacillus spp., in feed on growth rate and survival rate of snake-head fish (Channa striata). Snake-head fishes with the range of initial weights during 2.50-3.50 g were raised in 250-liter fiberglass tanks, 3 replications per treatment. Bacillus spp. (3.0×107 CFU/g) in different levels as 0, 2, 4 and 6 g were added to 1 kg of commercial diet to feed fish and then he fished were offered he diet for 60 days. The result showed that using 6 g/kg feed of Bacillus spp. was the best treatment for increasing the average of weight gain, length gain and growth rate of the studied fishes as 6.62±0.44 g/fish, 3.13±0.45 cm/fish and 2.06±0.02 %/day, respectively, with a statistically significant difference (P<0.05). However, it was not found the difference of the survival rate. The result of water quality analysis exhibited no any statistically significant difference among the studied groups (P>0.05). In conclusion, using 6 g/kg feed of Bacillus spp. as feed additive on snake-head fish culture was the best choice for increasing weight gain, length gain, and growth rate of snake-head fishes.
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