A Comparison of 3 Anesthetic Agents (MS-222, Benzocaine, and Clove oil) in East Asian Bullfrog (Hoplobatrachus rugulosus)

Article Sidebar

Full text
Published: Jun 22, 2020
Keywords:
East Asian Bullfrogs MS-222 Benzocaine Clove oil

Main Article Content

Wanna Sirimanapong
Suchada Saetang
Kittikul Namwongprom
Choenkwan Pabutta
Taweeporn Mitprayoon
Piyawan Kaewkhao
Sureerat Mitemit

Abstract

The objective of this study was to comparison of 3 anesthetic agents in East Asian Bullfrog (Hoplobatrachus rugulosus). The experiment was laid in a non-parametric statistic. Using 30 male , 4 months old East Asian Bullfrogs for sample groups The East Asian Bullfrogs were divided into 3 groups and randomly divided into 3 treatments; tricaine methanesulfonate (MS-222), benzocaine and clove oil. Using efficient recommended dose is 1,000 ppm, 300 ppm and 350 ppm respectively. Times were count when dip the East Asian Bullfrog in anesthetic agent until the frogs were full recovery to determine induction, duration and recovery time. The results showed that there were significant differences on frog’s anesthesia in each anesthetic agent (P<0.05). Tricaine methanesulfonate (MS-222) has short period about induction and duration time approximately 8.00 and 50.00 minutes respectively. While recovery time, clove oil has short period about 5.50 minutes. The recovery time in clove oil group was significant difference from benzocaine group (P<0.05) but not significant difference from MS-222 group (P>0.05).

Article Details

How to Cite
Sirimanapong, W., Saetang, S., Namwongprom, K., Pabutta, C., Mitprayoon, T., Kaewkhao, P. and Mitemit, S. (2020) “A Comparison of 3 Anesthetic Agents (MS-222, Benzocaine, and Clove oil) in East Asian Bullfrog (Hoplobatrachus rugulosus)”, Journal of Mahanakorn Veterinary Medicine, 15(1), pp. 43–55. Available at: https://li01.tci-thaijo.org/index.php/jmvm/article/view/240036 (Accessed: 13 November 2024).
Issue
Vol. 15 No. 1 (2020): January - June
Section
Research Article

References

Baorong, G. and C. Mingzhang. 1994. A Study of the Feeding and Breeding Habits of Rana tigerina rugulosa. J. Fujian. Teachers Univ. http://en.cnki.com.cn/Article_en/CJFDTotal-FJSZ403.016.htm

Cakir, Y. and S. M. Strauch. 2005. Tricaine (MS-222) is a safe anesthetic compound compared to benzocaine and pentobarbital to induce anesthesia in leopard frogs (Rana pipiens). Pharmacol. Rep. 57: 467-474.

Carpenter, J. W. 2013. Exotic Animal Formulary. 4th ed. Elsevier Health Sciences. 63-66.

Downes, H. 1995. Tricaine anesthesia in Amphibia: a review. Bull. Assoc. Rep. Amp. Vet. 5: 11-16.

Goulet, F., P. Hélie, and P. Vachon. 2010. Eugenol anesthesia in African clawed frogs (Xenopus laevis) of different body weights. J. AM. Assoc. Lab. Ani. Sci. 49: 460-463.

Goulet, F., P. Vachon, and P. Helie. 2011. Evaluation of the toxicity of eugenol at anesthetic doses in African clawed frogs (Xenopus laevis). Toxicol. Pathol. 39: 471-477.

Guénette, S. A., P. Hélie, F. Beaudry, and P. Vachon. 2007. Eugenol for anesthesia of African clawed frogs (Xenopus laevis). Vet. Anaesth. Analg. 34: 164-170.

Guenette, S. A., Lair, S. 2006. Anesthesia of the leopard frog, Rana pipiens: a comparative study between four different agents. J. Herpetol. Med. Sur. 16: 38-44.

Javahery, S., H. Nekoubin, and A. H. Moradlu. 2012. Effect of anesthesia with clove oil in fish. Fish. Physiol. Biochem. 38: 1545-1552.

Lalonde-Robert, V., F. Beaudry, and P. Vachon. 2012. Pharmacologic parameters of MS-222 and physiologic changes in frogs (Xenopus laevis) after immersion at anesthetic doses. J. AM. Assoc. Lab. Ani. Sci. 51: 464-468.

Mitchell, M. A. 2009. Anesthetic considerations for amphibians. J. Exot. Pet. Med. 18: 40-49.

Ramlochansingh, C., F. Branoner, B. P. Chagnaud, and H. Straka. 2014. Efficacy of tricaine methanesulfonate (MS-222) as an anesthetic agent for blocking sensory-motor responses in Xenopus laevis tadpoles. PloS One. 9: e101606. doi: 10.1371/journal.pone.0101606.

Ross, A., S. A. Guenette, P. Helie, and P. Vachon. 2006. Case of cutaneous necrosis in African clawed frogs Xenopus laevis after the topical application of eugenol. Can. Vet. J. 47: 1115-1117.

Smith, B. D., K. J. Vail, G. L. Carroll, M. C. Taylor, N. D. Jeffery, T. H. Vemulapalli, and J. J. Elliott. 2018. Comparison of etomidate, benzocaine, and MS222 anesthesia with and without subsequent flunixin meglumine analgesia in African clawed frogs (Xenopus laevis). J. Am. Assoc. Lab. Anim. Sci. 57: 202-209.

Steetter, M. D. 2001. Fish and amphibian anesthesia. Vet. Clin. North. Am. Exot. Anim. Pract. 4: 69-82.

Topic Popovic, N., I. Strunjak-Perovic, R. Coz-Rakovac, J. Barisic, M. Jadan, A. Persin Berakovic, and R. Sauerborn Klobucar. 2012. Tricaine methane-sulfonate (MS-222) application in fish anaesthesia. J. Appl. Ichthyol. 28: 553-564.