A Comparison of 3 Anesthetic Agents (MS-222, Benzocaine, and Clove oil) in East Asian Bullfrog (Hoplobatrachus rugulosus)

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Wanna Sirimanapong
Suchada Saetang
Kittikul Namwongprom
Choenkwan Pabutta
Taweeporn Mitprayoon
Piyawan Kaewkhao
Sureerat Mitemit


The objective of this study was to comparison of 3 anesthetic agents in East Asian Bullfrog (Hoplobatrachus rugulosus). The experiment was laid in a non-parametric statistic. Using 30 male , 4 months old East Asian Bullfrogs for sample groups The East Asian Bullfrogs were divided into 3 groups and randomly divided into 3 treatments; tricaine methanesulfonate (MS-222), benzocaine and clove oil. Using efficient recommended dose is 1,000 ppm, 300 ppm and 350 ppm respectively. Times were count when dip the East Asian Bullfrog in anesthetic agent until the frogs were full recovery to determine induction, duration and recovery time. The results showed that there were significant differences on frog’s anesthesia in each anesthetic agent (P<0.05). Tricaine methanesulfonate (MS-222) has short period about induction and duration time approximately 8.00 and 50.00 minutes respectively. While recovery time, clove oil has short period about 5.50 minutes. The recovery time in clove oil group was significant difference from benzocaine group (P<0.05) but not significant difference from MS-222 group (P>0.05).


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