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Seawater is generally resource of water for brackish or marine fish aquaculture. The natural seawater composed of many microorganism’s cause of disease in aquaculture. This study aimed to compare efficiency of chlorine at various concentrations (0, 5, 10, 20, 30, 40, 50 and 60 ppm) to kill bacteria in seawater before culturing fingering giant perch (Lates calcarifer). The amount of bacteria in the seawater were significantly reduce in 10, 20, 30, 40, 50 and 60 ppm of chlorine concentrations (P<0.05). Moreover, 80% bactericidal effect was found in the treated seawater at 40, 50 and 60 ppm after 24 hours of water preparation. The effective bactericidal concentration (40, 50 and 60 ppm) of the seawater was used for culturing fingering giant perch for 24 hours. After 24 hours of fish culturing, the number of bacteria increased in the chlorine treated group when compared to the treated seawater before fish culturing (P<0.05). Therefore, the optimal concentration for killing bacteria in seawater before culturing giant perch was 60 ppm when compared to the number of bacteria in the untreated natural seawater with significantly different (P<0.05). Since, it eliminated more than 99% bacteria in the seawater. More than 24 hours treated seawater should be consideration for culturing giant perch to prevent chlorine toxicity.
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