Preliminary study of Hair Cortisol Concentration (HCC) of Dogs before and after Surgery ็Hair cortisol concentration

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Kochakorn Direksin
Thanyaluk Srirat
Siwanart Juiboot
Soraya Khongthananet
Natthakorn Chaiyasaen
Suttisak Nopwinyoowong


This study aimed to investigate hair cortisol concentration (HCC) of dogs pre- and post-surgery. Hair samples were cut at the scruff area of a dog in the specific days. Four healthy dogs were rotationally undergone 7 types of surgery, hair samples were collected on Day0 (before) and Day1-6. Hair samples of four non-surgical dogs were collected on Day1-35 (to pre-evaluate HCC baseline). HCC in the hair was determined by Radioimmunoassay kit (CORT-CT2 Test kit, Cisbio Bioassays Model24). HCC before/after surgery and between surgery types were similar. Non-surgical dogs had average HCC of 5.3 ± 0.2 (4.2-8.4) pg/mg.  Mean HCC of surgical dogs, pre-/post-operation were 5.8 ± 0.9 (4-7.3)/6.2 ± 0.5 (5.9-10.8) pg/mg. HCC results from an individual dog indicate that HCC surged in operations those; 1) incision of many areas, 2) Enterectomy in which dogs were fasted for a week, and 3) Caudal midline approach laparotomy as compared to Paracostal incision.  From our results, HCC is a promising tool for stress and pain assessments.


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