The Effect of Moringa oleifera Lam. Extracts on Antibacterial Activity against Staphylococcus aureus from Bovine Mastitis

Main Article Content

Duangkamol Taemchuay
Krongkarn Phulub
Wimonmat Boonmee
Kanokpon Bunya-atichart


Staphylococcus aureus is a contagious pathogen as a cause of clinical and subclinical mastitis in dairy cows. The economic disease is affected to decrease milk productivity and milk quality. The antibiotic treatment is not successfully because S. aureus can resistance and penetration in tissue of the mammary gland. In addition, the used of antibiotic treatment has been causing antibiotic residue in milk and milk products. Therefore, traditional herbs are interested in a new alternative treatment from natural resources. Moringa oleifera Lam. is the Thai herb that has a wide range of medical properties for treatment of bovine mastitis. This research aimed to evaluate antibacterial effect of M. oleifera Lam. crude extracts against S. aureus and S. aureus ATCC 25923. Fresh of leaves, flowers and pod pulp and powder of leaf and pod pulp of M. oleifera Lam. were extracted by ethanol. All crude extracts were tested for antibacterial activity using by disc diffusion and broth dilution methods as reported by the minimum inhibitory concentration (MIC) values and the minimum bactericidal concentration (MBC) values.  The highest yield by ethanol extraction of fresh leaves was 9.30%. The disc diffusion method result showed that the inhibition zones of crude extract from fresh leaves at 20 mg/disc against S. aureus and S. aureus ATCC 25923 were 12.83±1.47 mm. and 14.67±0.52 mm., respectively. The MIC and MBC values of S.aureus were range 4-64 mg/ml and 32-64 mg/ml, respectively. This research indicates that the M. oleifera Lam. crude extracts prohibited the growth of S. aureus from mastitis in dairy cows.


Download data is not yet available.

Article Details

Research Article


Abdull, R., F. Ahmad, M.D. Ibrahim, and S.B. Kntayya. 2014. Health benefits of Moringa oleifera, Asian Pac. J. Cancer Prev. 15:8571–8576.

Ademiluyi, A.O., O.H. Aladeselu, G. Oboh, and A.A. Boligon. 2018. Drying alters the phenolic constituents, antioxidant properties, α-amylase, and α-glucosidase inhibitory properties of Moringa (Moringa oleifera) leaf, Food Sci. Nutr. 6:2123–2133.

Barkema, H.W., Y.H. Schukken, and R.N. Zadoks. 2006. Invited review: the role of cow, pathogen and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis, J Dairy Sci. 89:1877-1895.

Blowey RW. 2010. Mastitis control in dairy herds. 2nd ed. Cambridge, Mass, MA: CABI Dego, O. K., J.E. Van Duk, and H. Nederbragt. 2002. A review: factors involved in the early pathogenesis of bovine Staphylococcus aureus mastitis with emphasis on bacterial adhesion and invasion, Veterinary quartery. 24:182-198.

Douglas, D. B. and J. W. Robert. 2005. A novel strategy for the prevention of Staphylococcus aureus induced mastitis in dairy cows. n.p.

Erskine, R. J. 2001. Mastitis control in dairy herds, In O. M. Radostits, editor, Herd health: food animal production medicine. 3rd ed. W. B. Saunders Co., Philadelphia, PA. p. 397-433.

Forsback, L., H. Lindmark-Mansson, A. Andren, M. Akerstedt, and K. Svennersten-Sjaunja. 2009. Udder quarter milk composition at different levels of somatic cell count in cow composite milk, Journal Animal. 3(5):710-717.

Fouad, E.A., A.S.M. Abu Elnaga, and M.M. Kandil. 2019. Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess, Veterinary World. 12(6):802-808. (Abstract)

Gopalakrishnan, L., K. Doriya, and D.S. Kumar. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application, Food Sci. Hum. Wellness. 5:49–56.

Harmon R. 1994. Physiology of mastitis and factors affecting somatic cell counts, Journal of Dairy Science. 77:2103-2112

Kalpana, S., S. Moorthi, and K. Sushila. 2013. Antimicrobial activity of different extracts of leaf of Moringa oleifera (Lam) against gram positive and gram negative bacteria, Int. J. Curr. Microbiol. App. Sci. 2(12):514-518.

National Mastitis Council Research Committee Report (NMC). 1996. Milk quality and mastitis control in Canada: progress and outlook. NMC Annual Meeting proceedings.

Opara, A.U., G. Anyanwu, and G.C. Uloneme. 2015. The antibacterial action of Moringa oleifera on some wound and enteric pathogens, J. Med. Sci. Clin. Res. 3(7):6741-6757.

Pantosti, A., A. Sanchini, and M. Monaco. 2007. Mechanisms of antibiotic resistance in Staphylococcus aureus, Future Microbiology. 2:323-334.

Petrovski, K., M. Trajcev, and G. Buneski. 2006. A review of the factors affecting the costs of bovine mastitis, Journal of the South African Veterinary Association. 77:52-60.

Roberson, J.R., L.K. Fox, D.D Hancock, G.M. Gay, and T.E. Besser. 1994. Ecology of Staphylococcus aureus isolated from various sites on dairy farms, J. Dairy. Sci. 77(11):3354-3364.

Tirado Torres, D., C. A. Chan-Keb, R. A. Pérez-Balán, B. Ake-Canché, M. I. Gómez-Solano, J. L. Aragón-Gastélum, I. Gómez-López, F. J. Aguirre-Crespo, M. C. López-Ramos, and E. J. Gutiérrez-Alcántara. 2018. Antimicrobial activity of Moringa Oleifera against multidrug-resistant Staphylococcus Aureus isolated from raw milk, Applied Ecology and Environmental Research 17(1):587-599.

Zaku, S., S. Emmanuel, A. Tukur, and A. Kabir. 2015. Moringa oleifera: An underutilized tree in Nigeria with amazing versatility: A review. Afr. J. Food Sci. 9:456–461.