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Staphylococcus aureus is a contagious pathogen as a cause of clinical and subclinical mastitis in dairy cows. The economic disease is affected to decrease milk productivity and milk quality. The antibiotic treatment is not successfully because S. aureus can resistance and penetration in tissue of the mammary gland. In addition, the used of antibiotic treatment has been causing antibiotic residue in milk and milk products. Therefore, traditional herbs are interested in a new alternative treatment from natural resources. Moringa oleifera Lam. is the Thai herb that has a wide range of medical properties for treatment of bovine mastitis. This research aimed to evaluate antibacterial effect of M. oleifera Lam. crude extracts against S. aureus and S. aureus ATCC 25923. Fresh of leaves, flowers and pod pulp and powder of leaf and pod pulp of M. oleifera Lam. were extracted by ethanol. All crude extracts were tested for antibacterial activity using by disc diffusion and broth dilution methods as reported by the minimum inhibitory concentration (MIC) values and the minimum bactericidal concentration (MBC) values. The highest yield by ethanol extraction of fresh leaves was 9.30%. The disc diffusion method result showed that the inhibition zones of crude extract from fresh leaves at 20 mg/disc against S. aureus and S. aureus ATCC 25923 were 12.83±1.47 mm. and 14.67±0.52 mm., respectively. The MIC and MBC values of S.aureus were range 4-64 mg/ml and 32-64 mg/ml, respectively. This research indicates that the M. oleifera Lam. crude extracts prohibited the growth of S. aureus from mastitis in dairy cows.
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