The Comparative Study of Cortisol Extraction in Cow's Milk by Different Extract

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Sopita Suttikrai
Ratree Jintana
Tiprawee Rassamepong
Nitira Anakkul


Direct measurement of cortisol in milk interfered with milk lipids. Hormone extraction is essential prior before the measure by using the radioimmunoassay technique. This experiment was designed to study the different extracts: Dichloromethane, Diethyl ether, and Petroleum benzene for cortisol extraction in cow's milk by using different quantities of milk and dilution of the extract. From the results, it was found that the different quantities of milk on radioimmunoassay affect cortisol extraction. The extraction of 500 µl of milk provides a low recovery percentage (% Recovery) in dichloromethane and diethyl ether extract. Also, the quantities of milk per extract affect cortisol extraction. Dichloromethane extract yielded the highest recovery percentage by diluting 1: 24 and 1: 26 in diethyl ether extract. However, petroleum benzene extract for cortisol extraction was disappointing.


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Butler, W., & Bordes, C. D. 1980. Radioimmunoassay technique for measuring cortisol in milk. J. Dairy Sci, 63, 474-477.

Foroutan, A., Guo, A. C., Vazquez-Fresno, R., Lipfert, M., Zhang, L., Zheng, J., Wishart, D. S. 2019. Chemical composition of commercial cow’s milk. Journal of Agricultural and Food Chemistry, 67 , 4897-4914.

Forslund, K. B., Ljungvall, Ö. A., & Jones, B. V. 2010. Low cortisol levels in blood from dairy cows with ketosis: a field study. Acta Veterinaria Scandinavica, 52:31.

Ito, T., Aoki, N., Tsuchiya, A., Kaneko, S., Akiyama, K., Uetake, K., & Suzuki5, K. 2017. Detection of stress hormone in the milk for animal welfare using QCM method. Journal of Sensors, Article ID 6486891.

Kamonpatana, M., Luvira, Y., Bodhipaksha, P., & Kunawongkrit, A. 1976. Serum progesterone, 17-hydroxyprogesterone and 17 β-oestradiol during the oestrous cycle in the swamp buffalo in Thailand. Nuclear techniques in animal production and health as related to the soil-plant system. IAEA, Vienna, 569-577.

Matraszek-Zuchowska, I., Wozniak, B., & Posyniak, A. 2017. Determination of hormones residues in milk by gas Chromatography-Mass Spectrometry. Food Analytical Methods, 10, 727-739.

Mehta, B. M. 2015. Chemical composition of milk and milk products. Springer-Verlag Berlin Heidelberg.

Sgorlon, S., Fanzago, M., Guiatti, D., Gabai, G., Stradaioli, G., & Stefanon, B. 2015. Factors affecting milk cortisol in mid lactating dairy cows. BMC Veterinary Research, 11:259.