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Honey is a natural product enriched in multiple nutrients and provides energy to body cells. The objective of this study was to investigate the effects of honey supplementation in soy lecithin-based extender on both cooled and frozen semen preservation of fighting bulls. Semen collected using electro-ejaculation technique was cooled at 5°C in soy lecithin extender containing 0%, 1%, 2%, 4% and 8% (vol/vol) honey. Semen from the same bulls was frozen at -196°C in the extender containing 0%, 1% and 2% (vol/vol) honey. Semen quality parameters including percentage of total motility, progressive motility, and viability, were assessed on day 1, 3 and 5 post cooling, and on day 7 post freezing. The results showed that the percentage of total and progressive motility remained higher in the extender containing 2% honey for up to 3 days of chilling compared with the extender containing 8% honey and control. Adding honey to frozen sperm, on the other hand, had no effect. Therefore, this study suggests that 2% honey supplemented with soy lecithin extender can be used as an additional source of energy to improve sperm motility in the cool preservation of fighting bull semen.
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