Appropriated Honey Concentration Supplementation in Soy Lecithin Extender as an Additive Source of Energy to Improve Motility of Cool-Stored Fighting Bull Spermatozoa

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Wipawee Saengsoi
Kosit Areekit

Abstract

Honey is a natural product enriched in multiple nutrients and provides energy to body cells. The objective of this study was to investigate the effects of honey supplementation in soy lecithin-based extender on both cooled and frozen semen preservation of fighting bulls. Semen collected using electro-ejaculation technique was cooled at 5°C in soy lecithin extender containing 0%, 1%, 2%, 4% and 8% (vol/vol) honey. Semen from the same bulls was frozen at -196°C in the extender containing 0%, 1% and 2% (vol/vol) honey. Semen quality parameters including percentage of total motility, progressive motility, and viability, were assessed on day 1, 3 and 5 post cooling, and on day 7 post freezing. The results showed that the percentage of total and progressive motility remained higher in the extender containing 2% honey for up to 3 days of chilling compared with the extender containing 8% honey and control. Adding honey to frozen sperm, on the other hand, had no effect. Therefore, this study suggests that 2% honey supplemented with soy lecithin extender can be used as an additional source of energy to improve sperm motility in the cool preservation of fighting bull semen.

Article Details

How to Cite
Saengsoi, W. and Areekit, K. (2023) “Appropriated Honey Concentration Supplementation in Soy Lecithin Extender as an Additive Source of Energy to Improve Motility of Cool-Stored Fighting Bull Spermatozoa”, Journal of Mahanakorn Veterinary Medicine, 17(2), pp. 255–263. Available at: https://li01.tci-thaijo.org/index.php/jmvm/article/view/254775 (Accessed: 8 October 2024).
Section
Research Article

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